NCF2 (NM_001127651) Human Tagged ORF Clone
CAT#: RG225899
- TrueORF®
NCF2 (tGFP-tagged) - Human neutrophil cytosolic factor 2 (NCF2), transcript variant 2
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_001127651" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | NCF2 |
Synonyms | NCF-2; NOXA2; P67-PHOX; P67PHOX |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG225899 representing NM_001127651
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCTGGTGGAGGCCATCAGCCTCTGGAATGAAGGGGTGCTGGCAGCGGACAAGAAGGACTGGAAGG GAGCCCTGGATGCCTTCAGTGCCGTCCAGGACCCCCACTCCCGGATTTGCTTCAACATTGGCTGCATGTA CACTATCCTGAAGAACATGACTGAAGCAGAGAAGGCCTTTACCAGAAGCATTAACCGAGACAAGCACTTG GCAGTGGCTTACTTCCAACGAGGGATGCTCTACTACCAGACAGAGAAATATGATTTGGCTATCAAAGACC TTAAAGAAGCCTTGATTCAGCTTCGAGGGAACCAGCTGATAGACTATAAGATCCTGGGGCTCCAGTTCAA GCTGTTTGCCTGTGAGGTGTTATATAACATTGCTTTCATGTATGCCAAGAAGGAGGAATGGAAAAAAGCT GAAGAACAGTTAGCATTGGCCACGAGCATGAAGTCTGAGCCCAGACATTCCAAAATCGACAAGGCGATGG AGTGTGTCTGGAAGCAGAAGCTATATGAGCCAGTGGTGATCCCTGTGGGCAAGCTGTTTCGACCAAATGA GAGACAAGTGGCTCAGCTGGCCAAGAAGGATTACCTAGGCAAGGCGACGGTCGTGGCATCTGTGGTGGAT CAAGACAGTTTCTCTGGGTTTGCCCCTCTGCAACCACAGGCAGCTGAGCCTCCACCCAGACCGAAAACCC CAGAGATCTTCAGGGCTCTGGAAGGGGAGGCTCACCGTGTGCTATTTGGGTTTGTGCCTGAGACAAAAGA AGAGCTCCAGGTCATGCCAGGGAACATTGTCTTTGTCTTGAAGAAGGGCAATGATAACTGGGCCACGGTC ATGTTCAACGGGCAGAAGGGGCTTGTTCCCTGCAACTACCTTGAACCAGTTGAGCTGCGGATCCACCCTC AGCAGCAGCCCCAGGAGGAAAGCTCTCCGCAGTCCGACATCCCAGCTCCTCCTAGTTCCAAAGCCCCTGG AAGACCCCAGCTGTCACCAGGCCAGAAACAAAAAGAAGAGCCTAAGGAAGTGAAGCTCAGTGTTCCCATG CCCTACACACTCAAGGTGCACTACAAGTACACGGTAGTCATGAAGACTCAGCCCGGGCTCCCCTACAGCC AGGTCCGGGACATGGTGTCTAAGAAACTGGAGCTCCGGCTGGAACACACTAAGCTGAGCTATCGGCCTCG GGACAGCAATGAGCTGGTGCCCCTTTCAGAAGACAGCATGAAGGATGCCTGGGGCCAGGTGAAAAACTAC TGCCTGACTCTGTGGTGTGAGAACACAGTGGGTGACCAAGGCTTTCCAGATGAACCCAAGGAAAGTGAAA AAGCTGATGCTAATAACCAGACAACAGAACCTCAGCTTAAGAAAGGCAGCCAAGTGGAGGCACTCTTCAG TTATGAGGCTACCCAACCAGAGGACCTGGAGTTTCAGGAAGGGGATATAATCCTGGTGTTATCAAAGGTG AATGAAGAATGGCTGGAAGGGGAGTGCAAAGGGAAGGTGGGCATTTTCCCCAAAGTTTTTGTTGAAGACT GCGCAACTACAGATTTGGAAAGCACTCGGAGAGAAGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG225899 representing NM_001127651
Red=Cloning site Green=Tags(s) MSLVEAISLWNEGVLAADKKDWKGALDAFSAVQDPHSRICFNIGCMYTILKNMTEAEKAFTRSINRDKHL AVAYFQRGMLYYQTEKYDLAIKDLKEALIQLRGNQLIDYKILGLQFKLFACEVLYNIAFMYAKKEEWKKA EEQLALATSMKSEPRHSKIDKAMECVWKQKLYEPVVIPVGKLFRPNERQVAQLAKKDYLGKATVVASVVD QDSFSGFAPLQPQAAEPPPRPKTPEIFRALEGEAHRVLFGFVPETKEELQVMPGNIVFVLKKGNDNWATV MFNGQKGLVPCNYLEPVELRIHPQQQPQEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPM PYTLKVHYKYTVVMKTQPGLPYSQVRDMVSKKLELRLEHTKLSYRPRDSNELVPLSEDSMKDAWGQVKNY CLTLWCENTVGDQGFPDEPKESEKADANNQTTEPQLKKGSQVEALFSYEATQPEDLEFQEGDIILVLSKV NEEWLEGECKGKVGIFPKVFVEDCATTDLESTRREV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001127651 |
ORF Size | 1578 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001127651.3 |
RefSeq Size | 2256 bp |
RefSeq ORF | 1581 bp |
Locus ID | 4688 |
UniProt ID | P19878 |
Cytogenetics | 1q25.3 |
Protein Families | Druggable Genome |
Protein Pathways | Leukocyte transendothelial migration |
Gene Summary | This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC225899 | NCF2 (Myc-DDK-tagged)-Human neutrophil cytosolic factor 2 (NCF2), transcript variant 2 |
USD 490.00 |
|
RC225899L1 | Lenti ORF clone of Human neutrophil cytosolic factor 2 (NCF2), transcript variant 2, Myc-DDK-tagged |
USD 790.00 |
|
RC225899L2 | Lenti ORF clone of Human neutrophil cytosolic factor 2 (NCF2), transcript variant 2, mGFP tagged |
USD 790.00 |
|
RC225899L3 | Lenti ORF clone of Human neutrophil cytosolic factor 2 (NCF2), transcript variant 2, Myc-DDK-tagged |
USD 790.00 |
|
RC225899L4 | Lenti ORF clone of Human neutrophil cytosolic factor 2 (NCF2), transcript variant 2, mGFP tagged |
USD 790.00 |
|
SC323119 | NCF2 (untagged)-Human neutrophil cytosolic factor 2 (NCF2), transcript variant 2 |
USD 540.00 |
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