PHF20 (NM_016436) Human Tagged ORF Clone

CAT#: RG223059

PHF20 (tGFP-tagged) - Human PHD finger protein 20 (PHF20)

Product Images

Circular map for RG223059

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PHF20
• Synonyms: C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223059 representing NM_016436
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAAGCATCCACCTAACAGACGAGGAATCAGCTTTGAAGTGGGAGCCCAGTTGGAAGCCCGGGACC
  GTTTAAAAAACTGGTATCCAGCTCACATAGAAGACATTGACTACGAGGAAGGAAAAGTACTCATCCATTT
  CAAGCGTTGGAACCATCGTTATGATGAGTGGTTCTGCTGGGACAGTCCTTATTTACGCCCTTTAGAGAAA
  ATACAGCTGAGGAAAGAGGGCTTGCATGAAGAGGATGGATCTTCTGAATTTCAAATAAATGAGCAGGTCC
  TTGCTTGCTGGTCTGATTGTCGTTTTTACCCGGCCAAAGTCACTGCTGTTAACAAGGATGGTACTTACAC
  TGTGAAATTTTATGATGGAGTAGTTCAGACTGTCAAACATATTCATGTCAAAGCTTTTTCCAAAGATCAG
  AATATTGTGGGTAATGCTAGGCCTAAAGAAACAGATCACAAAAGTCTTTCATCATCTCCTGATAAACGAG
  AGAAGTTTAAAGAACAGAGAAAAGCAACAGTGAATGTGAAGAAAGACAAAGAAGATAAACCCTTAAAGAC
  AGAAAAGCGACCCAAGCAGCCTGATAAAGAAGGAAAGTTAATCTGTTCTGAAAAGGGGAAAGTGTCAGAG
  AAAAGTCTTCCCAAGAACGAGAAGGAAGACAAGGAAAACATTTCCGAAAATGACAGAGAGTATTCTGGAG
  ATGCCCAAGTGGATAAGAAACCTGAAAATGACATTGTGAAGAGTCCACAAGAAAACTTGAGGGAACCCAA
  AAGAAAACGAGGCAGACCCCCTTCCATAGCTCCTACTGCTGTGGATTCAAACTCTCAAACTTTGCAACCA
  ATAACATTGGAACTGAGAAGAAGGAAAATATCAAAAGGATGTGAAGTCCCATTAAAACGTCCTCGGCTTG
  ACAAAAATTCATCCCAGGAAAAGTCAAAAAACTACTCGGAAAACACTGACAAAGACTTATCGAGGAGACG
  TTCCTCCAGGCTGTCCACTAATGGGACCCATGAGATCCTAGATCCTGACTTGGTTGTATCAGATTTGGTT
  GATACGGATCCTTTGCAAGACACGTTGTCTAGTACCAAGGAATCTGAAGAAGGTCAGTTGAAGTCTGCTT
  TGGAAGCTGGCCAGGTCTCATCTGCACTGACTTGCCACTCCTTTGGGGATGGATCCGGGGCTGCAGGCTT
  GGAGTTGAACTGCCCATCAATGGGAGAAAACACGATGAAAACAGAACCGACTTCTCCCCTTGTGGAATTA
  CAAGAGATTTCGACTGTGGAAGTAACAAATACTTTTAAGAAAACAGATGATTTTGGGTCATCTAATGCAC
  CAGCTGTCGACCTAGACCATAAGTTTAGATGCAAAGTTGTGGACTGTTTAAAATTTTTCCGCAAAGCCAA
  ACTGTTGCACTATCACATGAAGTATTTCCATGGAATGGAGAAGTCACTGGAGCCAGAAGAGAGCCCGGGA
  AAGAGGCATGTCCAAACCAGGGGCCCTTCAGCTTCAGACAAGCCCAGCCAGGAGACCCTGACCAGGAAGC
  GGGTCTCTGCCAGTTCCCCAACTACAAAAGACAAGGAAAAGAATAAAGAGAAGAAATTCAAGGAGTTTGT
  GAGAGTGAAGCCAAAGAAGAAAAAGAAAAAGAAAAAGAAAACCAAACCTGAATGCCCCTGCAGTGAGGAG
  ATCAGTGACACCTCCCAGGAACCTTCTCCACCCAAGGCATTTGCTGTTACCAGGTGTGGGTCCTCACACA
  AGCCAGGGGTCCATATGAGCCCGCAGCTTCATGGCCCAGAATCTGGACACCACAAAGGGAAAGTGAAAGC
  ATTGGAGGAGGATAATTTGAGTGAGTCCTCTTCTGAGAGCTTTCTCTGGAGTGATGATGAGTATGGCCAA
  GATGTGGATGTGACCACCAACCCAGATGAGGAACTTGATGGGGATGACCGCTATGACTTCGAGGTGGTCC
  GCTGCATCTGTGAGGTCCAGGAGGAAAATGACTTCATGATTCAGTGTGAAGAGTGCCAGTGCTGGCAGCA
  TGGGGTCTGCATGGGATTACTGGAAGAAAATGTGCCCGAGAAATACACCTGTTATGTTTGCCAAGACCCT
  CCAGGTCAGAGGCCTGGCTTCAAGTACTGGTATGACAAGGAGTGGCTGAGCAGGGGACATATGCATGGCC
  TGGCATTTCTAGAAGAGAACTACTCCCATCAGAATGCCAAGAAGATCGTGGCCACCCACCAGCTTCTTGG
  TGATGTGCAGAGAGTGATTGAGGTTCTGCATGGCCTGCAGCTCAAGATGAGCATCTTGCAAAGCCGGGAG
  CATCCTGATCTGCCGCTGTGGTGCCAGCCTTGGAAACAGCACTCAGGGGAGGGGAGATCTCATTTCAGAA
  ACATCCCTGTCACTGACACCAGGAGCAAGGAGGAAGCTCCAAGCTATAGAACTTTGAACGGGGCAGTGGA
  GAAGCCCAGGCCCCTGGCCCTGCCCCTGCCGCGTTCTGTGGAGGAATCCTATATCACCAGTGAGCATTGC
  TACCAGAAGCCCCGCGCCTATTACCCTGCCGTGGAGCAGAAGCTGGTGGTGGAGACGAGGGGCTCTGCCC
  TCGACGATGCGGTCAACCCCCTCCATGAGAACGGCGATGATTCCCTTTCCCCGCGCCTGGGCTGGCCTCT
  AGACCAAGACAGGAGCAAGGGGGACAGTGACCCCAAACCCGGCTCCCCAAAGGTGAAGGAATATGTCTCC
  AAAAAGGCCCTACCAGAAGAAGCCCCTGCTCGGAAGCTGCTGGACAGAGGTGGAGAGGGGCTGCTGAGCT
  CCCAGCACCAGTGGCAGTTTAACCTGCTGACCCATGTGGAATCTCTTCAGGATGAAGTTACGCACAGGAT
  GGACTCCATTGAGAAGGAGTTGGATGTGTTGGAGAGCTGGCTGGACTACACTGGGGAACTGGAGCCCCCT
  GAGCCGCTGGCCAGGCTTCCGCAGCTCAAGCATTGTATCAAGCAGCTGCTGATGGACCTGGGCAAGGTGC
  AGCAGATCGCCCTCTGCTGCTCAACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223059 representing NM_016436
  Red=Cloning site Green=Tags(s)
  
  MTKHPPNRRGISFEVGAQLEARDRLKNWYPAHIEDIDYEEGKVLIHFKRWNHRYDEWFCWDSPYLRPLEK
  IQLRKEGLHEEDGSSEFQINEQVLACWSDCRFYPAKVTAVNKDGTYTVKFYDGVVQTVKHIHVKAFSKDQ
  NIVGNARPKETDHKSLSSSPDKREKFKEQRKATVNVKKDKEDKPLKTEKRPKQPDKEGKLICSEKGKVSE
  KSLPKNEKEDKENISENDREYSGDAQVDKKPENDIVKSPQENLREPKRKRGRPPSIAPTAVDSNSQTLQP
  ITLELRRRKISKGCEVPLKRPRLDKNSSQEKSKNYSENTDKDLSRRRSSRLSTNGTHEILDPDLVVSDLV
  DTDPLQDTLSSTKESEEGQLKSALEAGQVSSALTCHSFGDGSGAAGLELNCPSMGENTMKTEPTSPLVEL
  QEISTVEVTNTFKKTDDFGSSNAPAVDLDHKFRCKVVDCLKFFRKAKLLHYHMKYFHGMEKSLEPEESPG
  KRHVQTRGPSASDKPSQETLTRKRVSASSPTTKDKEKNKEKKFKEFVRVKPKKKKKKKKKTKPECPCSEE
  ISDTSQEPSPPKAFAVTRCGSSHKPGVHMSPQLHGPESGHHKGKVKALEEDNLSESSSESFLWSDDEYGQ
  DVDVTTNPDEELDGDDRYDFEVVRCICEVQEENDFMIQCEECQCWQHGVCMGLLEENVPEKYTCYVCQDP
  PGQRPGFKYWYDKEWLSRGHMHGLAFLEENYSHQNAKKIVATHQLLGDVQRVIEVLHGLQLKMSILQSRE
  HPDLPLWCQPWKQHSGEGRSHFRNIPVTDTRSKEEAPSYRTLNGAVEKPRPLALPLPRSVEESYITSEHC
  YQKPRAYYPAVEQKLVVETRGSALDDAVNPLHENGDDSLSPRLGWPLDQDRSKGDSDPKPGSPKVKEYVS
  KKALPEEAPARKLLDRGGEGLLSSQHQWQFNLLTHVESLQDEVTHRMDSIEKELDVLESWLDYTGELEPP
  EPLARLPQLKHCIKQLLMDLGKVQQIALCCST
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016436
• ORF Size: 3036 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016436.5
• RefSeq Size: 5583 bp
• RefSeq ORF: 3039 bp
• Locus ID: 51230
• UniProt ID: Q9BVI0
• Cytogenetics: 20q11.22-q11.23
• Domains: PHD, TUDOR, zf-C2H2
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Methyllysine-binding protein, component of the MOF histone acetyltransferase protein complex. Not required for maintaining the global histone H4 'Lys-16' acetylation (H4K16ac) levels or locus specific histone acetylation, but instead works downstream in transcriptional regulation of MOF target genes (By similarity). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. Contributes to methyllysine-dependent p53/TP53 stabilization and up-regulation after DNA damage.[UniProtKB/Swiss-Prot Function]