MYO1D (NM_015194) Human Tagged ORF Clone

CAT#: RG222422

MYO1D (tGFP-tagged) - Human myosin ID (MYO1D)

Product Images

Circular map for RG222422

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MYO1D
• Synonyms: myr4; PPP1R108
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222422 representing NM_015194
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCAGGAGAGCCTGGAATTCGGCAAGGCAGACTTCGTGCTGATGGACACCGTCTCCATGCCCG
  AGTTCATGGCCAACCTCAGGCTCAGATTTGAAAAAGGGCGCATCTATACGTTCATTGGAGAAGTCGTCGT
  TTCTGTGAACCCTTACAAGTTGTTGAACATCTATGGAAGAGACACAATTGAGCAGTATAAAGGCCGTGAG
  CTGTATGAGAGACCGCCTCACCTTTTTGCTATTGCGGATGCTGCTTACAAGGCTATGAAGAGGCGATCAA
  AAGACACTTGTATTGTGATATCAGGGGAAAGTGGAGCTGGTAAAACGGAAGCCAGTAAGTACATTATGCA
  GTATATTGCGGCCATCACCAACCCCAGTCAGAGAGCAGAGGTTGAAAGAGTGAAGAATATGTTGCTTAAG
  TCCAACTGTGTTTTGGAAGCTTTTGGAAATGCCAAAACCAACCGTAATGACAACTCAAGCAGGTTTGGAA
  AATACATGGATATCAACTTTGACTTCAAGGGTGACCCTATTGGTGGGCATATCAATAACTACTTACTAGA
  AAAGTCTCGAGTGATTGTGCAACAGCCAGGAGAAAGAAGCTTTCATTCTTTCTATCAGCTACTCCAAGGA
  GGTTCAGAACAAATGCTACGCTCTCTACATCTCCAGAAATCCCTTTCATCCTACAACTATATTCATGTGG
  GAGCTCAATTAAAGTCTTCTATCAATGATGCTGCCGAATTCAGAGTTGTTGCTGATGCCATGAAAGTCAT
  TGGCTTCAAACCTGAGGAGATCCAAACAGTGTATAAGATTTTGGCTGCTATTCTGCACTTGGGAAATTTA
  AAATTTGTAGTAGATGGTGACACGCCTCTTATTGAGAATGGCAAAGTAGTATCTATCATAGCAGAATTGC
  TCTCTACTAAGACAGATATGGTTGAGAAAGCCCTTCTTTACCGGACTGTGGCCACAGGCCGTGACATCAT
  TGACAAGCAGCACACAGAACAAGAGGCCAGCTACGGCAGAGACGCCTTTGCCAAGGCAATATATGAGCGC
  CTTTTTTGTTGGATCGTTACTCGCATCAATGATATTATTGAGGTCAAGAACTATGACACCACAATCCATG
  GGAAAAACACTGTTATTGGTGTCTTGGATATCTATGGCTTTGAAATCTTCGACAACAACAGTTTTGAACA
  ATTCTGTATCAATTACTGCAATGAGAAACTGCAGCAGCTATTTATTCAGCTGGTTCTGAAGCAAGAACAA
  GAGGAATACCAGCGGGAAGGGATCCCCTGGAAACATATTGACTACTTCAACAATCAGATCATTGTTGACC
  TCGTGGAGCAACAGCACAAAGGGATCATTGCAATCCTTGATGATGCTTGCATGAATGTCGGCAAAGTCAC
  CGATGAAATGTTTCTTGAAGCACTTAACAGTAAATTGGGCAAACACGCCCATTTTTCCAGCCGAAAGCTC
  TGTGCCTCAGACAAAATTCTGGAGTTTGATCGAGATTTTCGAATTCGACATTATGCAGGCGATGTAGTCT
  ATTCTGTCATTGGTTTTATTGACAAAAATAAAGATACTTTATTTCAAGATTTCAAGCGCCTTATGTATAA
  CAGTTCAAATCCTGTGCTCAAGAATATGTGGCCTGAAGGCAAACTGAGCATTACAGAGGTGACCAAGCGA
  CCTCTGACTGCTGCTACCTTGTTTAAGAATTCTATGATTGCTCTAGTAGACAACCTTGCATCAAAGGAAC
  CATATTACGTTCGTTGCATCAAACCCAATGACAAGAAATCTCCACAGATATTTGATGATGAACGCTGCCG
  GCACCAAGTAGAATATCTTGGACTACTGGAAAATGTGAGAGTGCGTCGGGCAGGATTTGCCTTCCGCCAG
  ACATACGAGAAGTTTCTTCACAGGTATAAGATGATCTCTGAATTCACCTGGCCCAACCATGACCTTCCTT
  CAGACAAAGAGGCTGTCAAGAAACTAATTGAACGGTGTGGTTTTCAGGATGATGTAGCTTATGGGAAGAC
  CAAAATTTTCATTCGAACACCCCGAACATTGTTTACCTTGGAAGAACTCCGTGCCCAGATGCTCATAAGG
  ATTGTCCTCTTTCTACAAAAGGTGTGGCGGGGCACCCTGGCCCGCATGCGGTACAAAAGAACCAAGGCAG
  CTCTGACAATAATCAGGTACTACCGGCGCTACAAAGTGAAGTCGTACATCCACGAGGTGGCCAGACGCTT
  CCATGGCGTCAAGACCATGCGAGACTACGGGAAGCACGTGAAGTGGCCAAGCCCTCCTAAAGTTCTTCGC
  CGTTTTGAGGAGGCCCTGCAGACGATTTTCAATAGATGGAGAGCATCCCAGCTCATCAAGAGCATTCCGG
  CCTCAGACCTGCCCCAGGTCAGGGCAAAGGTTGCAGCCGTGGAAATGTTGAAGGGTCAAAGGGCTGACCT
  CGGGCTCCAGAGGGCCTGGGAGGGCAACTATCTTGCTTCAAAGCCAGATACACCTCAGACCTCAGGCACT
  TTTGTCCCTGTTGCTAATGAATTGAAACGGAAGGACAAATACATGAATGTCCTCTTTTCCTGTCACGTCC
  GTAAGGTAAATCGATTTAGTAAGGTGGAAGACAGAGCAATTTTTGTCACTGACCGTCACCTGTATAAAAT
  GGATCCCACTAAACAGTACAAGGTGATGAAGACTATCCCTCTATACAATTTGACTGGTCTGAGTGTCTCC
  AATGGAAAGGACCAACTTGTAGTGTTCCATACGAAAGACAACAAAGACCTCATTGTCTGCCTCTTCAGCA
  AACAGCCAACCCATGAGAGTCGAATTGGAGAACTTGTTGGAGTGCTGGTGAATCATTTCAAGAGTGAGAA
  GCGCCACCTTCAAGTGAACGTCACCAACCCAGTACAGTGCAGCCTGCACGGGAAGAAGTGCACCGTCTCC
  GTGGAGACGCGGCTCAACCAGCCCCAGCCCGACTTCACCAAGAATCGCTCGGGCTTCATCCTCAGCGTGC
  CCGGGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222422 representing NM_015194
  Red=Cloning site Green=Tags(s)
  
  MAEQESLEFGKADFVLMDTVSMPEFMANLRLRFEKGRIYTFIGEVVVSVNPYKLLNIYGRDTIEQYKGRE
  LYERPPHLFAIADAAYKAMKRRSKDTCIVISGESGAGKTEASKYIMQYIAAITNPSQRAEVERVKNMLLK
  SNCVLEAFGNAKTNRNDNSSRFGKYMDINFDFKGDPIGGHINNYLLEKSRVIVQQPGERSFHSFYQLLQG
  GSEQMLRSLHLQKSLSSYNYIHVGAQLKSSINDAAEFRVVADAMKVIGFKPEEIQTVYKILAAILHLGNL
  KFVVDGDTPLIENGKVVSIIAELLSTKTDMVEKALLYRTVATGRDIIDKQHTEQEASYGRDAFAKAIYER
  LFCWIVTRINDIIEVKNYDTTIHGKNTVIGVLDIYGFEIFDNNSFEQFCINYCNEKLQQLFIQLVLKQEQ
  EEYQREGIPWKHIDYFNNQIIVDLVEQQHKGIIAILDDACMNVGKVTDEMFLEALNSKLGKHAHFSSRKL
  CASDKILEFDRDFRIRHYAGDVVYSVIGFIDKNKDTLFQDFKRLMYNSSNPVLKNMWPEGKLSITEVTKR
  PLTAATLFKNSMIALVDNLASKEPYYVRCIKPNDKKSPQIFDDERCRHQVEYLGLLENVRVRRAGFAFRQ
  TYEKFLHRYKMISEFTWPNHDLPSDKEAVKKLIERCGFQDDVAYGKTKIFIRTPRTLFTLEELRAQMLIR
  IVLFLQKVWRGTLARMRYKRTKAALTIIRYYRRYKVKSYIHEVARRFHGVKTMRDYGKHVKWPSPPKVLR
  RFEEALQTIFNRWRASQLIKSIPASDLPQVRAKVAAVEMLKGQRADLGLQRAWEGNYLASKPDTPQTSGT
  FVPVANELKRKDKYMNVLFSCHVRKVNRFSKVEDRAIFVTDRHLYKMDPTKQYKVMKTIPLYNLTGLSVS
  NGKDQLVVFHTKDNKDLIVCLFSKQPTHESRIGELVGVLVNHFKSEKRHLQVNVTNPVQCSLHGKKCTVS
  VETRLNQPQPDFTKNRSGFILSVPGN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015194
• ORF Size: 3018 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015194.1, NP_056009.1
• RefSeq Size: 5182 bp
• RefSeq ORF: 3021 bp
• Locus ID: 4642
• UniProt ID: O94832
• Cytogenetics: 17q11.2
• Gene Summary: Unconventional myosin that functions as actin-based motor protein with ATPase activity (By similarity). Plays a role in endosomal protein trafficking, and especially in the transfer of cargo proteins from early to recycling endosomes (By similarity). Required for normal planar cell polarity in ciliated tracheal cells, for normal rotational polarity of cilia, and for coordinated, unidirectional ciliary movement in the trachea. Required for normal, polarized cilia organization in brain ependymal epithelial cells (By similarity).[UniProtKB/Swiss-Prot Function]