HS3ST6 (NM_001009606) Human Tagged ORF Clone

CAT#: RG222279

HS3ST6 (tGFP-tagged) - Human heparan sulfate (glucosamine) 3-O-sulfotransferase 6 (HS3ST6)

Product Images

Circular map for RG222279

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HS3ST6
• Synonyms: 3-OST-6; HS3ST5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222279 representing NM_001009606
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTCGTGGCCCTGGTGCTCGGCGCCTACTGCCTCTGCGCCCTCCCCGGCCGCTGCCCGCCGGCCG
  CCCGCGCCCCCGCGCCGGCCCCCGCGCCCTCCGAGCCGTCCAGCTCCGTCCACCGCCCGGGAGCACCCGG
  CCTGCCTTTGGCCAGCGGTCCCGGCCGCCGGCGCTTCCCGCAAGCGCTCATCGTTGGCGTGAAGAAGGGC
  GGCACGCGCGCCCTGCTGGAGTTTCTGCGGCTGCACCCCGACGTCCGCGCGCTGGGCTCTGAGCCCCACT
  TCTTCGACAGGTGCTACGAGCGCGGCCTCGCCTGGTACCGGAGTCTGATGCCCCGAACCCTGGATGGGCA
  GATCACCATGGAGAAGACCCCCAGCTACTTCGTGACGCGAGAGGCCCCCCGCCGCATCCACGCCATGTCC
  CCGGACACGAAGCTGATCGTGGTGGTGCGGAACCCCGTGACCCGGGCCATCTCCGACTATACCCAGACGC
  TCTCCAAGACCCCGGGCCTGCCCAGCTTCCGCGCCCTGGCCTTCCGCCACGGCCTGGGCCCCGTGGACAC
  AGCCTGGAGCGCCGTCCGCATCGGCCTGTACGCCCAGCACCTGGACCACTGGCTGCGCTACTTCCCCCTG
  TCCCACTTCCTGTTCGTCAGCGGGGAGCGTCTGGTCAGCGACCCGGCCGGAGAGGTCGGCCGCGTGCAGG
  ACTTCCTGGGCCTGAAACGGGTCGTCACGGACAAGCACTTCTACTTCAACGCCACCAAGGGCTTCCCCTG
  CCTCAAGAAGGCCCAGGGCGGCAGCCGTCCCCGCTGCCTGGGCAAGTCCAAGGGCCGGCCACACCCACGC
  GTGCCCCAGGCCGTGGTCCGGCGCCTGCAGGAGTTCTACCGGCCCTTCAACCGCAGGTTCTACCAGATGA
  CGGGCCAGGACTTCGGCTGGGGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222279 representing NM_001009606
  Red=Cloning site Green=Tags(s)
  
  MLLVALVLGAYCLCALPGRCPPAARAPAPAPAPSEPSSSVHRPGAPGLPLASGPGRRRFPQALIVGVKKG
  GTRALLEFLRLHPDVRALGSEPHFFDRCYERGLAWYRSLMPRTLDGQITMEKTPSYFVTREAPRRIHAMS
  PDTKLIVVVRNPVTRAISDYTQTLSKTPGLPSFRALAFRHGLGPVDTAWSAVRIGLYAQHLDHWLRYFPL
  SHFLFVSGERLVSDPAGEVGRVQDFLGLKRVVTDKHFYFNATKGFPCLKKAQGGSRPRCLGKSKGRPHPR
  VPQAVVRRLQEFYRPFNRRFYQMTGQDFGWG
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009606
• ORF Size: 936 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009606.1, NP_001009606.1
• RefSeq Size: 936 bp
• RefSeq ORF: 1029 bp
• Locus ID: 64711
• UniProt ID: Q96QI5
• Cytogenetics: 16p13.3
• Gene Summary: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to heparan sulfate. The substrate-specific O-sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes Simplex Virus-1 (HSV-1) and permits its entry. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate.[UniProtKB/Swiss-Prot Function]