EXOC3L4 (NM_001077594) Human Tagged ORF Clone

CAT#: RG222102

EXOC3L4 (tGFP-tagged) - Human exocyst complex component 3-like 4 (EXOC3L4)

Product Images

Circular map for RG222102

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EXOC3L4
• Synonyms: C14orf73
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222102 representing NM_001077594
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCATCACCACAGACAGACACTCCTGGGCCGGAGCTGCAGAGTCCCAAGGAGGCTGAGGAGCCACAGA
  CTCCAGCTCAGGGCTCCCGGCGAACAAGCAGCAGGAAAGAGCCCAATGCCCACCGCAAGGATGGCACAAG
  GCTGGGCCTGGGCTCCCTGAGGCAGGCCTTCTCCCGGGCCAGCCAGCGGGCTTTGACCCAGGTCTCCAAG
  GAAGATACGGGCCTGTTCCGGCGAAGCTCCTGCTCCCTGTTCCGGTCCTTCCGGCAAGCCCTGAATGACG
  GCCCAGCTACCGGCCATTCCCAGGCCACTCCTGAGGTGCCCTCGGGGGTCATGAATGGTGTCAGCCAGCA
  GGCATCCACTGGGGCAGCGTCTGAGGAACTGAAACCCGAGGCAGAAGGCAAATCCGTGGCCGACCTCATT
  ACTGAACGGCAACTGCTGGCGGCCTTCGAACAGCTTCTGCGCCTGGAGACGCTGCTGGTGGCCGAGAAGG
  CCTCGCGCACCTTTGAGCAGGACCCTACGGCCTTCGCGCGGCGCGCTATGGACGTGTGCCTGCTTTACGA
  CGGGCTGGCAGCCGAGATCGGCGCCATCGTGCGCGAGACGCTGGACAGCGACGGTGTGGACGCGGCCGCG
  CTGGCCGAGCTGGCCCGCGTGGTGAGCGCGGAGGAGGAAGCCCACCCTTCTCCCCCCGACGACGGCGACT
  TCCTGCGCACGCCGCGCCGCTGGCGCCAGCACTGGGAGGAGGCGGTGCGGCGAAGCGCTCAGGAGCGCGT
  GCGGCGGCCGGGCGCGGGGTGGGCCTTCGGGGAGGCGGAGGGCGCGTCGGGTTTGGCCCAGCTTCTGGCC
  GAGCTGGGTGGCTTGGTTCGCCGCGACCTGCAGAAGGTGCGGCAGGAGGTGCAGCCCGCGTATGCGGCGG
  CCGGCTTCCCAGCGTGGGAGGTCTATCTGCGTGCCTTCCACAGCGCCGTGGCCCAGCGCCTCCAGGAGCT
  CGCGCGCGACGCCCGCGGCTGCGAGCAGCTCTATATCCTGCTGGACTGGGCCGCCAACGTCTACGGCAGT
  CCTGACTTCCTGGGCGCCCCGGGGCTGGCGCTGCCCGCCGAGCCGCTGCCTCCGCTCCTGGCGCCGGACG
  TGTGGGCCCGACTGGAGAGCGACTACACCAGCTTCCTGGAGGCCAAGATCGCAAGCTGCTTCGACAGCAT
  CTTGCAGCTGGAGCAGAGTCACTGGGCGGCCGCCGAGGTCCCCGAGGTGCTGCAGGGCCTCTACCAGGCG
  CCGCTGTCCATGGACGTCCATATGCTCGTGGCCGAGCACGTGAAGGCGGCCGGCGCCATCTCCGCGGAGC
  TGGAGGCCACCACCCTGCGAATCTGCACGCGGGCGCTCGGCCTCTTCGTGCCCAGGTTTGAAAAGGCTTT
  TCTGGCGTCGGAGGCGGTGAGCGAGCCGCACCTGGGCGCCTACATCAACGCCTGCGAGGAGCTCAGGACC
  AGTCTTCTCTCCAGGTTCCCAGGAACCCAAGAGGAGCTGGAGAAGCCCCTGGTGACGGCCACCTGCAGCT
  TCCAGAAGCACTTGCTTCAGGGCTTGCAGCGTGAGTTGCAGCCGCTCTTCAGGGTTGTGTGCACCAGGGA
  CTGGCTGACGCAGGACTGGCTGCATCCCCTCATGGACAAGGTGGTGACCTTCGCCGGTCATCTCCAGCGT
  GTGGCCCGGCCGCGGGCACAGGAGACTCTGCAGGAGGTGCACCGGTTCGTGGTCCGCGAGTACCTGGCGC
  GGGCGCTGAGGCCACGGGAGCGGTTCCGGGGCATGGAGCGCATGCATGGCTCCCAGAAGATGAGCCTGGA
  TGCCCAGGCCATCAGCGACACCTTCCAGGGCCTGGGTTCCGAGGCCACATGGTTGGACCAAGCCATCCAG
  TGCGTGGCTGAGATCCTGGGCGAGACCTACAAAGATGACATCCAGCGGCACCTGGAGACTCTTATCCGGA
  GCTACCCCGACATCAGGCGGGACCACATACTGGCCATTCTGGCGCTGCGCCGACTGGGCCGCCAGCGGAA
  CCAGCATCTCTTGCAGCACACTCAAGACCTGCTGAGAGCTGCGGCCGGGGCGGCGGGTGCGGAGGCCCCT
  CGGGGCCGCGTGCTCTTCGAGGAGATCAAGGTGCCCAGTGCCATGGCTGTGCTGATCACCTGCGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222102 representing NM_001077594
  Red=Cloning site Green=Tags(s)
  
  MPSPQTDTPGPELQSPKEAEEPQTPAQGSRRTSSRKEPNAHRKDGTRLGLGSLRQAFSRASQRALTQVSK
  EDTGLFRRSSCSLFRSFRQALNDGPATGHSQATPEVPSGVMNGVSQQASTGAASEELKPEAEGKSVADLI
  TERQLLAAFEQLLRLETLLVAEKASRTFEQDPTAFARRAMDVCLLYDGLAAEIGAIVRETLDSDGVDAAA
  LAELARVVSAEEEAHPSPPDDGDFLRTPRRWRQHWEEAVRRSAQERVRRPGAGWAFGEAEGASGLAQLLA
  ELGGLVRRDLQKVRQEVQPAYAAAGFPAWEVYLRAFHSAVAQRLQELARDARGCEQLYILLDWAANVYGS
  PDFLGAPGLALPAEPLPPLLAPDVWARLESDYTSFLEAKIASCFDSILQLEQSHWAAAEVPEVLQGLYQA
  PLSMDVHMLVAEHVKAAGAISAELEATTLRICTRALGLFVPRFEKAFLASEAVSEPHLGAYINACEELRT
  SLLSRFPGTQEELEKPLVTATCSFQKHLLQGLQRELQPLFRVVCTRDWLTQDWLHPLMDKVVTFAGHLQR
  VARPRAQETLQEVHRFVVREYLARALRPRERFRGMERMHGSQKMSLDAQAISDTFQGLGSEATWLDQAIQ
  CVAEILGETYKDDIQRHLETLIRSYPDIRRDHILAILALRRLGRQRNQHLLQHTQDLLRAAAGAAGAEAP
  RGRVLFEEIKVPSAMAVLITCV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077594
• ORF Size: 2166 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077594.1, NP_001071062.1
• RefSeq Size: 2591 bp
• RefSeq ORF: 2169 bp
• Locus ID: 91828
• UniProt ID: Q17RC7
• Cytogenetics: 14q32.32