HSZFP36 (ZNF823) (NM_001080493) Human Tagged ORF Clone

CAT#: RG221349

ZNF823 (tGFP-tagged) - Human zinc finger protein 823 (ZNF823)

Product Images

Circular map for RG221349

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HSZFP36
• Synonyms: HSZFP36
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221349 representing NM_001080493
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCAGTGGCCTTTGAAGATGTGGCTGTGAACTTCACACAAGAGGAGTGGGCTTTGCTGGGTCCAT
  CACAGAAGAGTCTCTACAGAAATGTCATGCAGGAAACCATTAGGAACCTGGACTGTATAGAAATGAAATG
  GGAGGACCAGAACATTGGAGATCAGTGCCAAAATGCCAAGAGAAATCTAAGAAGTCATACATGTGAAATT
  AAAGATGACAGTCAATGTGGAGAAACTTTTGGCCAGATTCCAGATAGTATTGTGAACAAGAACACTCCTC
  GAGTAAATCCATGTGACAGTGGTGAGTGTGGAGAAGTCGTCTTGGGTCATTCGTCTCTTAATTGCAACAT
  CAGAGTTGACACTGGACACAAATCATGTGAGCATCAGGAATATGGAGAGAAGCCATATACACATAAACAA
  CGTGGGAAAGCCATCAGTCATCAGCACTCCTTCCAGACACATGAAAGGCCCCCCACCGGAAAGAAACCCT
  TCGATTGTAAAGAATGTGCAAAAACCTTTAGTTCTCTTGGAAACCTCCGAAGACACATGGCGGCACACCA
  TGGAGATGGACCTTATAAATGTAAGTTGTGTGGGAAAGCCTTTGTTTGGCCCAGTTTATTTCATTTGCAC
  GAAAGAACACACACTGGAGAGAAACCGTATGAATGTAAGCAGTGTTCTAAAGCCTTTCCTTTTTACAGTT
  CCTATCTAAGACATGAAAGAATCCACACGGGAGAGAAAGCGTATGAATGTAAGCAGTGTTCCAAAGCCTT
  TCCTGATTACAGTACCTATCTAAGACATGAGAGAACTCACACCGGAGAGAAACCCTATAAATGTACACAA
  TGTGGGAAAGCCTTCAGCTGTTACTATTACACTCGACTACATGAAAGGACTCACACGGGAGAACAACCCT
  ATGCATGTAAGCAATGTGGGAAAACGTTTTATCATCACACAAGCTTTCGAAGACACATGATAAGGCACAC
  TGGAGACGGACCACATAAATGTAAGATATGTGGGAAAGGCTTTGATTGTCCTAGTTCAGTTCGAAATCAT
  GAAACTACTCACACTGGAGAGAAACCCTATGAATGTAAGCAGTGTGGGAAAGTGTTATCTCATAGCTCGA
  GCTTTCGAAGTCACATGATAACACACACAGGAGATGGACCCCAGAAATGCAAGATATGTGGGAAAGCCTT
  TGGTTGTCCCAGTTTATTTCAAAGACATGAAAGGACTCACACTGGAGAGAAACCCTATCAATGTAAACAA
  TGTGGTAAAGCCTTCAGTCTTGCCGGTTCCCTTCGAAGACATGAAGCAACTCACACTGGAGTGAAACCCT
  ATAAATGTCAGTGTGGGAAAGCCTTTAGTGATCTCTCTTCCTTTCAAAATCATGAGACAACTCACACTGG
  AGAGAAGCCATATGAGTGTAAGGAATGTGGGAAAGCATTCAGTTGTTTCAAATACCTTTCTCAACATAAA
  AGGACCCACACAGTAGAAAAACCTTATGAGTGTAAAACATGTAGAAAAGCCTTCAGTCATTTCAGTAACT
  TAAAAGTCCATGAAAGGATTCACTCTGGAGAGAAGCCATATGAATGTAAGGAATGTGGAAAAGCATTCTC
  TTGGCTCACTTGCCTTCTACGACATGAAAGAATTCACACTGGAGAGAAACCCTATGAATGTCTACAATGT
  GGTAAAGCCTTCACTCGTTCCCGTTTCCTTCGAGGACATGAAAAAACTCACACTGGAGAGAAGCTGTATG
  AATGTAAGGAATGTGGGAAAGCATTGAGTTCTCTCCGTTCCTTGCATAGACATAAAAGGACTCACTGGAA
  AGATACTCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221349 representing NM_001080493
  Red=Cloning site Green=Tags(s)
  
  MDSVAFEDVAVNFTQEEWALLGPSQKSLYRNVMQETIRNLDCIEMKWEDQNIGDQCQNAKRNLRSHTCEI
  KDDSQCGETFGQIPDSIVNKNTPRVNPCDSGECGEVVLGHSSLNCNIRVDTGHKSCEHQEYGEKPYTHKQ
  RGKAISHQHSFQTHERPPTGKKPFDCKECAKTFSSLGNLRRHMAAHHGDGPYKCKLCGKAFVWPSLFHLH
  ERTHTGEKPYECKQCSKAFPFYSSYLRHERIHTGEKAYECKQCSKAFPDYSTYLRHERTHTGEKPYKCTQ
  CGKAFSCYYYTRLHERTHTGEQPYACKQCGKTFYHHTSFRRHMIRHTGDGPHKCKICGKGFDCPSSVRNH
  ETTHTGEKPYECKQCGKVLSHSSSFRSHMITHTGDGPQKCKICGKAFGCPSLFQRHERTHTGEKPYQCKQ
  CGKAFSLAGSLRRHEATHTGVKPYKCQCGKAFSDLSSFQNHETTHTGEKPYECKECGKAFSCFKYLSQHK
  RTHTVEKPYECKTCRKAFSHFSNLKVHERIHSGEKPYECKECGKAFSWLTCLLRHERIHTGEKPYECLQC
  GKAFTRSRFLRGHEKTHTGEKLYECKECGKALSSLRSLHRHKRTHWKDTL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080493
• ORF Size: 1830 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080493.4
• RefSeq Size: 2368 bp
• RefSeq ORF: 1833 bp
• Locus ID: 55552
• UniProt ID: P16415
• Cytogenetics: 19p13.2
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]