ENTPD8 (NM_198585) Human Tagged ORF Clone
CAT#: RG220938
- TrueORF®
ENTPD8 (tGFP-tagged) - Human ectonucleoside triphosphate diphosphohydrolase 8 (ENTPD8), transcript variant 2
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_198585" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | ENTPD8 |
Synonyms | E-NTPDase; GLSR2492; NTPDase-8; UNQ2492 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG220938 representing NM_198585
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTGTCCCGGAAGGAGCAGGTCTTCTTGGCCCTGCTGGGGGCCTCGGGGGTCTCAGGCCTCACGG CACTCATTCTCCTCCTGGTGGAGGCCACCAGCGTGCTCCTGCCCACAGACATCAAGTTTGGGATCGTGTT TGATGCGGGCTCCTCCCACACGTCCCTCTTCCTGTATCAGTGGCCGGCGAACAAGGAGAATGGCACGGGT GTGGTCAGCCAGGCCCTGGCCTGCCAGGTGGAAGGGCCTGGAATCTCCTCCTACACTTCTAATGCTGCAC AGGCTGGTGAGAGCCTGCAGGGCTGCTTGGAGGAGGCGCTGGTGCTGATCCCAGAGGCCCAGCATCGGAA AACACCCACGTTCCTGGGGGCCACGGCTGGCATGAGGTTGCTCAGCCGGAAGAACAGCTCTCAGGCCAGG GACATCTTTGCAGCAGTCACCCAGGTCCTGGGCCGGTCTCCCGTGGACTTTTGGGGTGCCGAGCTCCTGG CCGGGCAGGCCGAAGGTGCCTTTGGTTGGATCACTGTCAACTACGGCTTGGGGACGCTGGTCAAGTACTC CTTCACTGGAGAATGGATCCAGCCTCCGGAGGAGATGCTGGTGGGTGCCCTGGACATGGGAGGGGCCTCC ACCCAGATCACGTTCGTGCCTGGGGGCCCCATCTTGGACAAGAGCACCCAGGCCGATTTTCGCCTCTACG GCTCCGACTACAGCGTCTACACTCACAGCTACCTGTGCTTTGGACGGGACCAGATGCTGAGCAGGCTCCT CGTGGGGCTGGTGCAGAGCCGCCCGGCTGCCCTGCTCCGTCACCCGTGCTACCTCAGCGGCTACCAGACC ACACTGGCCCTGGGCCCGCTGTATGAGTCACCCTGTGTCCACGCCACGCCCCCGCTGAGCCTCCCCCAGA ACCTCACAGTTGAAGGGACAGGCAACCCTGGAGCCTGCGTCTCAGCCATCCGGGAACTTTTCAACTTCTC CAGCTGCCAGGGCCAGGAGGACTGCGCCTTTGACGGGGTCTACCAGCCCCCGCTGCGGGGCCAGTTCTAT GTGGAGGCCAGCTACCCTGGGCAGGACCGCTGGCTGCGGGACTACTGTGCCTCAGGCCTGTACATCCTCA CCCTCCTGCACGAGGGCTACGGGTTCAGCGAGGAGACCTGGCCCAGCCTCGAGTTCCGAAAGCAGGCGGG CGGTGTGGACATTGGCTGGACACTGGGCTACATGCTGAACCTGACCGGGATGATCCCGGCCGATGCGCCG GCTCAGTGGCGGGCAGAGAGCTACGGCGTCTGGGTGGCCAAAGTGGTGTTCATGGTGCTGGCCCTGGTGG CGGTGGTGGGGGCTGCCTTGGTCCAGCTCTTCTGGTTGCAGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220938 representing NM_198585
Red=Cloning site Green=Tags(s) MGLSRKEQVFLALLGASGVSGLTALILLLVEATSVLLPTDIKFGIVFDAGSSHTSLFLYQWPANKENGTG VVSQALACQVEGPGISSYTSNAAQAGESLQGCLEEALVLIPEAQHRKTPTFLGATAGMRLLSRKNSSQAR DIFAAVTQVLGRSPVDFWGAELLAGQAEGAFGWITVNYGLGTLVKYSFTGEWIQPPEEMLVGALDMGGAS TQITFVPGGPILDKSTQADFRLYGSDYSVYTHSYLCFGRDQMLSRLLVGLVQSRPAALLRHPCYLSGYQT TLALGPLYESPCVHATPPLSLPQNLTVEGTGNPGACVSAIRELFNFSSCQGQEDCAFDGVYQPPLRGQFY VEASYPGQDRWLRDYCASGLYILTLLHEGYGFSEETWPSLEFRKQAGGVDIGWTLGYMLNLTGMIPADAP AQWRAESYGVWVAKVVFMVLALVAVVGAALVQLFWLQD TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_198585 |
ORF Size | 1374 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_198585.1, NP_940987.1 |
RefSeq Size | 2126 bp |
RefSeq ORF | 1377 bp |
Locus ID | 377841 |
UniProt ID | Q5MY95 |
Cytogenetics | 9q34.3 |
Protein Families | Transmembrane |
Protein Pathways | Purine metabolism, Pyrimidine metabolism |
Gene Summary | Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC220938 | ENTPD8 (Myc-DDK-tagged)-Human ectonucleoside triphosphate diphosphohydrolase 8 (ENTPD8), transcript variant 2 |
USD 457.00 |
|
RC220938L3 | Lenti-ORF clone of ENTPD8 (Myc-DDK-tagged)-Human ectonucleoside triphosphate diphosphohydrolase 8 (ENTPD8), transcript variant 2 |
USD 757.00 |
|
RC220938L4 | Lenti-ORF clone of ENTPD8 (mGFP-tagged)-Human ectonucleoside triphosphate diphosphohydrolase 8 (ENTPD8), transcript variant 2 |
USD 757.00 |
|
SC307761 | ENTPD8 (untagged)-Human ectonucleoside triphosphate diphosphohydrolase 8 (ENTPD8), transcript variant 2 |
USD 503.00 |
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