TEX13B (NM_031273) Human Tagged ORF Clone

CAT#: RG220609

TEX13B (tGFP-tagged) - Human testis expressed 13B (TEX13B)

Product Images

Circular map for RG220609

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TEX13B
• Synonyms: TGC3B; TSGA5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220609 representing NM_031273
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTGAGACCTGAGGACCCCAGTAGTGGGTTCCGGCACGGAAACGTGGTGGCCTTCATCATCGAGA
  AAATGGCCAGGCACACGAAAGGCCCCGAGTTCTACTTCGAGAATATATCCTTATCCTGGGAGGAGGTGGA
  AGACAAGCTCAGGGCCATCCTGGAGGACAGCGAGGTGCCCAGCGAGGTCAAAGAGGCCTGCACCTGGGGC
  AGCCTGGCCTTGGGTGTGCGCTTTGCCCACAGGCAGGGGCAGTTACAAAACCGCAGGGTGCAGTGGCTGC
  AAGGCTTTGCCAAACTGCACAGATCAGCTGCGCTGGTCTTGGCCTCAAACCTGACGGAACTCAAGGAACA
  GCAGGAGATGGAATGCAATGAGGCGACCTTCCAGTTGCAGCTAACCGAGACCAGCCTTGCGGAGGTGCAG
  AGAGAGCGGGACATGCTGAGATGGAAGCTCTTCCATGCCGAGCTGGCACCTCCCCAGGGACAGGGCCAGG
  CTACAGTGTTTCCAGGCCTGGCCACTGCCGGAGGGGATTGGACAGAAGGAGCAGGTGAGCAGGAAAAGGA
  GGCGGTGGCTGCTGCTGGTGCTGCTGGAGGAAAAGGAGAGGAGAGGTATGCAGAGGCAGGGCCTGCCCCC
  GCAGAGGTCTTGCAGGGGCTGGGAGGAGGCTTCAGGCAGCCCCTCGGAGCTATTGTAGCAGGCAAATTAC
  ACCTTTGCGGGGCAGAGGGAGAAAGATCTCAGGTCAGTACAAACAGCCATGTCTGTCTTCTCTGGGCTTG
  GGTCCACAGTCTCACTGGAGCCTCTTCCTGTCCAGCTCCCTACCTCATTCACATACTCATACCCATGCCC
  TTTGTCCGCCTTCTCAGCCATACCCAATATACCCCCTTCACCAGCAAAGGTCACAGAACGGGTTCCAACT
  CAGATGCCTTTCAACTGGGGGGCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220609 representing NM_031273
  Red=Cloning site Green=Tags(s)
  
  MALRPEDPSSGFRHGNVVAFIIEKMARHTKGPEFYFENISLSWEEVEDKLRAILEDSEVPSEVKEACTWG
  SLALGVRFAHRQGQLQNRRVQWLQGFAKLHRSAALVLASNLTELKEQQEMECNEATFQLQLTETSLAEVQ
  RERDMLRWKLFHAELAPPQGQGQATVFPGLATAGGDWTEGAGEQEKEAVAAAGAAGGKGEERYAEAGPAP
  AEVLQGLGGGFRQPLGAIVAGKLHLCGAEGERSQVSTNSHVCLLWAWVHSLTGASSCPAPYLIHILIPMP
  FVRLLSHTQYTPFTSKGHRTGSNSDAFQLGGL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031273
• ORF Size: 936 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031273.2, NP_112563.1
• RefSeq Size: 1247 bp
• RefSeq ORF: 939 bp
• Locus ID: 56156
• UniProt ID: Q9BXU2
• Cytogenetics: Xq22.3
• Gene Summary: This gene is similar to a mouse gene that is expressed in the testis. [provided by RefSeq, Jul 2008]