FSH Receptor (FSHR) (NM_181446) Human Tagged ORF Clone

CAT#: RG219729

FSHR (tGFP-tagged) - Human follicle stimulating hormone receptor (FSHR), transcript variant 2

Product Images

Circular map for RG219729

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FSH Receptor
• Synonyms: FSHR1; FSHRO; LGR1; ODG1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219729 representing NM_181446
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGCTCCTGGTCTCTTTGCTGGCATTCCTGAGCTTGGGCTCAGGATGTCATCATCGGATCTGTC
  ACTGCTCTAACAGGGTTTTTCTCTGCCAAGAGAGCAAGGTGACAGAGATTCCTTCTGACCTCCCGAGGAA
  TGCCATTGAACTGAGGTTTGTCCTCACCAAGCTTCGAGTCATCCAAAAAGGTGCATTTTCAGGATTTGGG
  GACCTGGAGAAAATAGAGATCTCTCAGAATGATGTCTTGGAGGTGATAGAGGCAGATGTGTTCTCCAACC
  TTCCCAAATTACATGAAATTAGAATTGAAAAGGCCAACAACCTGCTCTACATCAACCCTGAGGCCTTCCA
  GAACCTTCCCAACCTTCAATATCTGTTAATATCCAACACAGGTATTAAGCACCTTCCAGATGTTCACAAG
  ATTCATTCTCTCCAAAAAGTTTTACTTGACATTCAAGATAACATAAACATCCACACAATTGAAAGAAATT
  CTTTCGTGGGGCTGAGCTTTGAAAGTGTGATTCTATGGCTGAATAAGAATGGGATTCAAGAAATACACAA
  CTGTGCATTCAATGGAACCCAACTAGATGAGCTGAATCTAAGCGATAATAATAATTTAGAAGAATTGCCT
  AATGATGTTTTCCACGGAGCCTCTGGACCAGTCATTCTCTCTGAGCTTCATCCAATTTGCAACAAATCTA
  TTTTAAGGCAAGAAGTTGATTATATGACTCAGGCTAGGGGTCAGAGATCCTCTCTGGCAGAAGACAATGA
  GTCCAGCTACAGCAGAGGATTTGACATGACGTACACTGAGTTTGACTATGACTTATGCAATGAAGTGGTT
  GACGTGACCTGCTCCCCTAAGCCAGATGCATTCAACCCATGTGAAGATATCATGGGGTACAACATCCTCA
  GAGTCCTGATATGGTTTATCAGCATCCTGGCCATCACTGGGAACATCATAGTGCTAGTGATCCTAACTAC
  CAGCCAATATAAACTCACAGTCCCCAGGTTCCTTATGTGCAACCTGGCCTTTGCTGATCTCTGCATTGGA
  ATCTACCTGCTGCTCATTGCATCAGTTGATATCCATACCAAGAGCCAATATCACAACTATGCCATTGACT
  GGCAAACTGGGGCAGGCTGTGATGCTGCTGGCTTTTTCACTGTCTTTGCCAGTGAGCTGTCAGTCTACAC
  TCTGACAGCTATCACCTTGGAAAGATGGCATACCATCACGCATGCCATGCAGCTGGACTGCAAGGTGCAG
  CTCCGCCATGCTGCCAGTGTCATGGTGATGGGCTGGATTTTTGCTTTTGCAGCTGCCCTCTTTCCCATCT
  TTGGCATCAGCAGCTACATGAAGGTGAGCATCTGCCTGCCCATGGATATTGACAGCCCTTTGTCACAGCT
  GTATGTCATGTCCCTCCTTGTGCTCAATGTCCTGGCCTTTGTGGTCATCTGTGGCTGCTATATCCACATC
  TACCTCACAGTGCGGAACCCCAACATCGTGTCCTCCTCTAGTGACACCAGGATCGCCAAGCGCATGGCCA
  TGCTCATCTTCACTGACTTCCTCTGCATGGCACCCATTTCTTTCTTTGCCATTTCTGCCTCCCTCAAGGT
  GCCCCTCATCACTGTGTCCAAAGCAAAGATTCTGCTGGTTCTGTTTCACCCCATCAACTCCTGTGCCAAC
  CCCTTCCTCTATGCCATCTTTACCAAAAACTTTCGCAGAGATTTCTTCATTCTGCTGAGCAAGTGTGGCT
  GCTATGAAATGCAAGCCCAAATTTATAGGACAGAAACTTCATCCACTGTCCACAACACCCATCCAAGGAA
  TGGCCACTGCTCTTCAGCTCCCAGAGTCACCAGTGGTTCCACTTACATAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219729 representing NM_181446
  Red=Cloning site Green=Tags(s)
  
  MALLLVSLLAFLSLGSGCHHRICHCSNRVFLCQESKVTEIPSDLPRNAIELRFVLTKLRVIQKGAFSGFG
  DLEKIEISQNDVLEVIEADVFSNLPKLHEIRIEKANNLLYINPEAFQNLPNLQYLLISNTGIKHLPDVHK
  IHSLQKVLLDIQDNINIHTIERNSFVGLSFESVILWLNKNGIQEIHNCAFNGTQLDELNLSDNNNLEELP
  NDVFHGASGPVILSELHPICNKSILRQEVDYMTQARGQRSSLAEDNESSYSRGFDMTYTEFDYDLCNEVV
  DVTCSPKPDAFNPCEDIMGYNILRVLIWFISILAITGNIIVLVILTTSQYKLTVPRFLMCNLAFADLCIG
  IYLLLIASVDIHTKSQYHNYAIDWQTGAGCDAAGFFTVFASELSVYTLTAITLERWHTITHAMQLDCKVQ
  LRHAASVMVMGWIFAFAAALFPIFGISSYMKVSICLPMDIDSPLSQLYVMSLLVLNVLAFVVICGCYIHI
  YLTVRNPNIVSSSSDTRIAKRMAMLIFTDFLCMAPISFFAISASLKVPLITVSKAKILLVLFHPINSCAN
  PFLYAIFTKNFRRDFFILLSKCGCYEMQAQIYRTETSSTVHNTHPRNGHCSSAPRVTSGSTYI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181446
• ORF Size: 1870 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181446.1, NP_852111.1
• RefSeq Size: 2196 bp
• RefSeq ORF: 2010 bp
• Locus ID: 2492
• UniProt ID: P23945
• Cytogenetics: 2p16.3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: The protein encoded by this gene belongs to family 1 of G-protein coupled receptors. It is the receptor for follicle stimulating hormone and functions in gonad development. Mutations in this gene cause ovarian dysgenesis type 1, and also ovarian hyperstimulation syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]