ZNF300 (NM_052860) Human Tagged ORF Clone

CAT#: RG219156

ZNF300 (tGFP-tagged) - Human zinc finger protein 300 (ZNF300), transcript variant 2

Product Images

Circular map for RG219156

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF300
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219156 representing NM_052860
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAAGTCCCAGGGGTTAGTATCATTCAAGGATGTGGCTGTGGATTTCACCCAGGAGGAGTGGCAGC
  AACTTGACCCTTCTCAGAGGACCCTGTACAGGGATGTGATGCTGGAGAACTACAGCCACCTGGTCTCAAT
  GGGGTATCCAGTTTCCAAACCAGATGTCATCTCCAAGTTGGAACAAGGAGAAGAGCCATGGATCATAAAG
  GGAGACATATCAAATTGGATCTATCCAGATGAATATCAGGCAGATGGGAGACAAGACAGGAAGAGTAACC
  TTCACAACTCCCAGTCATGTATTTTGGGGACAGTTTCCTTCCATCATAAGATACTGAAAGGAGTCACAAG
  GGATGGTTCATTGTGCTCCATTTTAAAAGTCTGTCAAGGTGATGGTCAGCTGCAGAGATTTCTAGAGAAT
  CAAGACAAACTCTTCAGGCAGGTCACATTTGTTAACAGCAAAACAGTGACTGAGGCATCAGGGCATAAAT
  ATAATCCACTGGGGAAAATATTTCAAGAGTGCATAGAAACAGATATATCAATACAGAGATTCCATAAATA
  TGATGCTTTTAAAAAGAACTTAAAACCAAATATTGACCTACCGAGTTGTTATAAGAGCAATTCAAGAAAA
  AAACCTGATCAGAGTTTTGGAGGTGGAAAATCATCTAGCCAGAGTGAGCCCAATTCTAATCTTGAGAAGA
  TTCACAATGGAGTAATACCTTTTGATGATAATCAGTGTGGAAACGTTTTTAGAAATACACAATCCCTTAT
  TCAATATCAGAATGTGGAAACTAAAGAGAAAAGCTGTGTATGTGTTACATGTGGAAAAGCCTTTGCTAAG
  AAGTCACAACTCATTGTACATCAAAGAATTCATACTGGAAAGAAACCATATGATTGTGGTGCATGCGGAA
  AAGCCTTCAGTGAGAAGTTTCATCTTGTTGTACATCAGAGAACTCATACTGGGGAGAAACCTTATGATTG
  TTCTGAATGTGGAAAAGCCTTCTCTCAGAAATCGTCCCTTATTATACATCAGAGAGTTCACACTGGGGAA
  AAACCCTATGAATGTAGTGAATGCGGGAAAGCCTTCTCCCAGAAATCACCCCTCATTATACATCAGAGAA
  TACATACTGGGGAAAAACCCTATGAATGTAGAGAGTGTGGGAAGGCCTTTTCCCAGAAGTCACAGCTGAT
  TATACACCACAGAGCTCATACTGGAGAGAAGCCGTATGAGTGTACCGAATGTGGGAAAGCCTTCTGTGAG
  AAGTCCCACCTCATTATACATAAAAGAATTCACACTGGTGAGAAACCCTACAAATGTGCTCAATGTGAGG
  AAGCCTTCAGCAGGAAGACAGAACTCATTACACATCAGTTAGTTCATACTGGGGAAAAACCTTATGAATG
  TACTGAATGTGGAAAGACATTCTCCCGCAAGTCACAGCTCATCATACATCAGAGAACACATACTGGAGAA
  AAACCCTATAAATGTAGTGAATGTGGCAAAGCCTTCTGCCAGAAGTCACATCTCATTGGACATCAGAGAA
  TTCACACAGGAGAAAAACCTTATATATGTACTGAATGTGGGAAAGCCTTCTCTCAGAAGTCCCACCTTCC
  GGGACACCAGCGAATTCATACAGGAGAGAAACCTTACATATGTGCTGAATGTGGAAAGGCCTTTTCTCAG
  AAGTCAGACCTTGTTTTACATCAGAGGATTCATACTGGGGAAAGACCCTATCAATGTGCTATATGTGGGA
  AGGCCTTCATCCAGAAGTCACAACTAACTGTACACCAGAGAATTCACACAGTGGTAAAATCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219156 representing NM_052860
  Red=Cloning site Green=Tags(s)
  
  MMKSQGLVSFKDVAVDFTQEEWQQLDPSQRTLYRDVMLENYSHLVSMGYPVSKPDVISKLEQGEEPWIIK
  GDISNWIYPDEYQADGRQDRKSNLHNSQSCILGTVSFHHKILKGVTRDGSLCSILKVCQGDGQLQRFLEN
  QDKLFRQVTFVNSKTVTEASGHKYNPLGKIFQECIETDISIQRFHKYDAFKKNLKPNIDLPSCYKSNSRK
  KPDQSFGGGKSSSQSEPNSNLEKIHNGVIPFDDNQCGNVFRNTQSLIQYQNVETKEKSCVCVTCGKAFAK
  KSQLIVHQRIHTGKKPYDCGACGKAFSEKFHLVVHQRTHTGEKPYDCSECGKAFSQKSSLIIHQRVHTGE
  KPYECSECGKAFSQKSPLIIHQRIHTGEKPYECRECGKAFSQKSQLIIHHRAHTGEKPYECTECGKAFCE
  KSHLIIHKRIHTGEKPYKCAQCEEAFSRKTELITHQLVHTGEKPYECTECGKTFSRKSQLIIHQRTHTGE
  KPYKCSECGKAFCQKSHLIGHQRIHTGEKPYICTECGKAFSQKSHLPGHQRIHTGEKPYICAECGKAFSQ
  KSDLVLHQRIHTGERPYQCAICGKAFIQKSQLTVHQRIHTVVKS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052860
• ORF Size: 1812 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052860.2, NP_443092.1
• RefSeq Size: 3282 bp
• RefSeq ORF: 1815 bp
• Locus ID: 91975
• UniProt ID: Q96RE9
• Cytogenetics: 5q33.1
• Domains: KRAB, zf-C2H2
• Protein Families: Transcription Factors
• Gene Summary: The protein encoded by this gene is a C2H2-type zinc finger DNA binding protein and likely transcriptional regulator. The function of this protein is not yet known. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]