COBLL1 (NM_014900) Human Tagged ORF Clone

CAT#: RG218913

COBLL1 (tGFP-tagged) - Human COBL-like 1 (COBLL1)

Product Images

Circular map for RG218913

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: COBLL1
• Synonyms: COBLR1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218913 representing NM_014900
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGCCGAACCCCGCGCCCGCAGGACGCCCCAGCCAGGAGAAAACCAAAAGCCAAGGCACCACTTC
  CTCCAGCTGAGACCAAATATACTGATGTCTCTTCAGCTGCTGATTCTGTAGAATCCACTGCTTTCATCAT
  GGAACAGAAAGAAAACATGATAGATAAAGACGTTGAACTCTCAGTGGTCCTACCTGGGGATATTATCAAA
  TCTACTACTGTTCATGGCAGTAAACCTATGATGGACTTGTTGATATTCCTTTGTGCACAGTATCACTTAA
  ATCCATCAAGTTACACAATCGATCTGTTGTCAGCTGAACAGAACCACATTAAATTTAAGCCAAACACACC
  AATAGGAATGTTGGAGGTAGAGAAGGTAATTTTAAAGCCAAAAATGTTGGATAAGAAAAAACCTACACCT
  ATAATACCAGAGAAAACTGTGAGAGTAGTGATTAATTTTAAGAAAACACAGAAGACCATAGTGAGAGTGA
  GTCCACATGCATCGCTTCAAGAGCTTGCCCCTATTATATGTAGCAAATGTGAGTTTGATCCGTTGCATAC
  ACTATTGTTGAAAGATTATCAATCGCAGGAGCCTCTTGACTTGACAAAATCTCTTAATGACCTGGGACTA
  AGAGAATTATATGCGATGGATGTCAACAGAGAGTCCTGCCAAATATCACAAAACCTAGATATTATGAAGG
  AGAAAGAAAATAAAGGGTTTTTCAGTTTTTTTCAACGCAGTAAGAAAAAGCGAGACCAAACTGCAAGTGC
  CCCTGCAACCCCTCTAGTAAATAAGCACCGCCCAACTTTTACAAGGTCCAATACCATTTCCAAACCATAT
  ATTTCCAACACCCTGCCGTCGGATGCACCCAAGAAGAGGCGGGCTCCACTGCCCCCGATGCCAGCATCTC
  AGAGTGTCCCCCAAGACCTTGCACACATCCAGGAGAGGCCTGCTTCTTGTATAGTGAAATCCATGAGCGT
  GGATGAGACAGATAAGAGTCCCTGTGAAGCAGGAAGAGTGAGGGCAGGTTCACTGCAGCTCAGCAGCATG
  TCTGCAGGGAATTCATCTTTGAGAAGGACAAAGCGAAAAGCACCTTCCCCACCCTCCAAAATACCCCCGC
  ATCAAAGTGATGAAAATAGTCGTGTGACTGCCTTACAGCCAGTAGATGGAGTTCCTCCAGACAGTGCTTC
  AGAAGCAAACTCTCCTGAGGAGCTATCCAGCCCAGAAACCTTTCACCCTGGGCTTTCCAGTCAGGAGCAG
  TGCACTGCGCCCAAACTGATGGAGGAAACCTCTGTCTTTGAGTGCCCTGGGACACCTGAGGCAGCCATAA
  CATCATTGACATCTGGAATAAGCTCTGATTATAGCCTTGAAGAGATAGATGAAAAGGAAGAACTGAGTGA
  AGTGCCTAAAGTTGAAGCTGAAAATATTTCTCCGAAGTCACAAGATATTCCTTTTGTATCTACTGATATA
  ATAAATACACTGAAAAATGATCCTGACTCAGCCCTTGGCAATGGTAGTGGAGAGTTCTCACAAAACTCCA
  TGGAAGAAAAACAAGAAACTAAAAGCACAGATGGACAAGAACCACACAGTGTAGTATATGATACAAGCAA
  TGGAAAGAAGGTAGTTGACAGTATAAGAAACTTGAAGTCGTTGGGCCCAAACCAAGAGAATGTTCAAAAT
  GAAATAATTGTCTATCCAGAGAACACAGAAGACAATATGAAAAATGGAGTGAAGAAAACAGAAATCAATG
  TAGAAGGTGTTGCCAAAAATAACAACATTGATATGGAAGTTGAGAGACCATCAAACTCTGAGGCACATGA
  AACTGATACTGCTATAAGTTACAAGGAAAACCATCTAGCAGCTTCATCAGTACCAGATCAAAAACTGAAT
  CAACCCAGTGCAGAAAAGACAAAAGATGCAGCAATTCAGACAACCCCTTCTTGTAACAGTTTTGATGGGA
  AACACCAAGATCATAATTTATCTGACTCCAAAGTTGAAGAATGTGTGCAAACTTCAAATAACAACATATC
  AACTCAACACTCATGCTTAAGTTCACAAGATTCTGTAAATACCTCAAGGGAATTCAGGAGTCAAGGCACC
  CTAATTATACATTCAGAAGATCCGCTTACCGTAAAAGATCCAATTTGTGCACATGGTAATGATGATCTTT
  TGCCTCCTGTAGATAGGATTGACAAAAATTCCACTGCTTCTTACCTAAAGAATTACCCACTTTATAGACA
  GGACTACAATCCCAAGCCAAAACCTTCAAATGAAATTACACGAGAGTATATACCCAAAATTGGCATGACT
  ACTTATAAAATAGTGCCTCCCAAATCCTTGGAAATATCGAAAGACTGGCAATCAGAAACCATAGAGTATA
  AAGATGATCAGGACATGCATGCTTTAGGGAAAAAGCACACTCATGAGAATGTGAAAGAAACTGCCATCCA
  AACAGAAGATTCTGCTATTTCTGAAAGCCCAGAAGAGCCACTGCCAAACCTTAAACCGAAGCCTAACCTG
  AGAACAGAGCATCAAGTGCCCAGTTCTGTGAGCTCACCTGATGATGCCATGGTTAGTCCTCTGAAACCTG
  CTCCCAAAATGACAAGAGACACTGGCACAGCTCCTTTTGCACCAAATTTGGAAGAAATAAACAATATTTT
  GGAATCAAAATTTAAATCTCGGGCTTCAAATGCCCAGGCCAAACCCAGCTCTTTTTTTTTGCAGATGCAG
  AAGAGAGTATCGGGTCACTATGTGACATCTGCAGCTGCCAAGAGTGTCCATGCTGCCCCTAATCCTGCTC
  CAAAAGAACTGACAAATAAAGAGGCAGAAAGGGATATGCTGCCTTCTCCGGAGCAGACTCTTTCTCCCTT
  AAGTAAAATGCCTCACTCTGTTCCACAACCCCTTGTTGAAAAAACTGATGATGATGTCATCGGTCAGGCT
  CCTGCTGAAGCCTCCCCTCCTCCCATAGCTCCAAAACCTGTGACAATTCCTGCTAGTCAGGTATCCACAC
  AAAATCTGAAGACTTTGAAAACTTTTGGTGCCCCACGACCATACTCAAGTTCTGGTCCTTCACCGTTTGC
  TCTTGCTGTAGTGAAAAGGTCACAGTCTTTCAGTAAAGAGCGCACCGAGTCACCTAGTGCCAGTGCATTG
  GTCCAACCTCCAGCCAACACAGAGGAAGGGAAGACTCATTCTGTAAATAAATTTGTGGACATCCCACAGC
  TTGGTGTGTCTGATAAGGAAAATAACTCTGCACATAATGAACAGAATTCCCAAATACCAACTCCAACTGA
  TGGCCCATCATTCACTGTTATGAGACAAAGTTCTTTAACATTCCAAAGCTCTGACCCAGAACAGATGCGA
  CAGAGTTTGCTGACTGCAATCCGTTCGGGAGAGGCTGCTGCCAAATTGAAAAGGGTTACCATTCCATCAA
  ATACAATATCTGTGAATGGAAGGTCAAGACTCAGCCATTCCATGTCCCCTGATGCCCAGGACGGCCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218913 representing NM_014900
  Red=Cloning site Green=Tags(s)
  
  MDGRTPRPQDAPARRKPKAKAPLPPAETKYTDVSSAADSVESTAFIMEQKENMIDKDVELSVVLPGDIIK
  STTVHGSKPMMDLLIFLCAQYHLNPSSYTIDLLSAEQNHIKFKPNTPIGMLEVEKVILKPKMLDKKKPTP
  IIPEKTVRVVINFKKTQKTIVRVSPHASLQELAPIICSKCEFDPLHTLLLKDYQSQEPLDLTKSLNDLGL
  RELYAMDVNRESCQISQNLDIMKEKENKGFFSFFQRSKKKRDQTASAPATPLVNKHRPTFTRSNTISKPY
  ISNTLPSDAPKKRRAPLPPMPASQSVPQDLAHIQERPASCIVKSMSVDETDKSPCEAGRVRAGSLQLSSM
  SAGNSSLRRTKRKAPSPPSKIPPHQSDENSRVTALQPVDGVPPDSASEANSPEELSSPETFHPGLSSQEQ
  CTAPKLMEETSVFECPGTPEAAITSLTSGISSDYSLEEIDEKEELSEVPKVEAENISPKSQDIPFVSTDI
  INTLKNDPDSALGNGSGEFSQNSMEEKQETKSTDGQEPHSVVYDTSNGKKVVDSIRNLKSLGPNQENVQN
  EIIVYPENTEDNMKNGVKKTEINVEGVAKNNNIDMEVERPSNSEAHETDTAISYKENHLAASSVPDQKLN
  QPSAEKTKDAAIQTTPSCNSFDGKHQDHNLSDSKVEECVQTSNNNISTQHSCLSSQDSVNTSREFRSQGT
  LIIHSEDPLTVKDPICAHGNDDLLPPVDRIDKNSTASYLKNYPLYRQDYNPKPKPSNEITREYIPKIGMT
  TYKIVPPKSLEISKDWQSETIEYKDDQDMHALGKKHTHENVKETAIQTEDSAISESPEEPLPNLKPKPNL
  RTEHQVPSSVSSPDDAMVSPLKPAPKMTRDTGTAPFAPNLEEINNILESKFKSRASNAQAKPSSFFLQMQ
  KRVSGHYVTSAAAKSVHAAPNPAPKELTNKEAERDMLPSPEQTLSPLSKMPHSVPQPLVEKTDDDVIGQA
  PAEASPPPIAPKPVTIPASQVSTQNLKTLKTFGAPRPYSSSGPSPFALAVVKRSQSFSKERTESPSASAL
  VQPPANTEEGKTHSVNKFVDIPQLGVSDKENNSAHNEQNSQIPTPTDGPSFTVMRQSSLTFQSSDPEQMR
  QSLLTAIRSGEAAAKLKRVTIPSNTISVNGRSRLSHSMSPDAQDGH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014900
• ORF Size: 3498 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014900.5
• RefSeq Size: 4923 bp
• RefSeq ORF: 3501 bp
• Locus ID: 22837
• UniProt ID: Q53SF7
• Cytogenetics: 2q24.3
• Domains: WH2