C16orf89 (NM_001098514) Human Tagged ORF Clone

CAT#: RG218396

C16orf89 (tGFP-tagged) - Human chromosome 16 open reading frame 89 (C16orf89), transcript variant 2

Product Images

Circular map for RG218396

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C16orf89
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218396 representing NM_001098514
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCCTGGGGCTGCTGCTCCTGCTCTTACTGACAGCACTGCCACCGCTGTGGTCCTCCTCACTGC
  CTGGGCTGGACACTGCTGAAAGTAAAGCCACCATTGCAGACCTGATCCTGTCTGCGCTGGAGAGAGCCAC
  CGTCTTCCTAGAACAGAGGCTGCCTGAAATCAACCTGGATGGCATGGTGGGGGTCCGAGTGCTGGAAGAG
  CAGCTAAAAAGTGTCCGGGAGAAGTGGGCCCAGGAGCCCCTGCTGCAGCCGCTGAGCCTGCGCGTGGGGA
  TGCTGGGGGAGAAGCTGGAGGCTGCCATCCAGAGATCCCTCCACTACCTCAAGCTGAGTGATCCCAAGTA
  CCTAAGAGAGTTCCAGCTGACCCTCCAGCCCGGGTTTTGGAAGCTCCCACATGCCTGGATCCACACTGAT
  GCCTCCTTGGTGTACCCCACGTTCGGGCCCCAGGACTCATTCTCAGAGGAGAGAAGTGACGTGTGCCTGG
  TGCAGCTGCTGGGAACCGGGACGGACAGCAGCGAGCCCTGCGGCCTCTCAGACCTCTGCAGGAGCCTCAT
  GACCAAGCCCGGCTGCTCAGGCTACTGCCTGTCCCACCAACTGCTCTTCTTCCTCTGGGCCAGAATGAGG
  GGGTGCACACAGGGACCACTCCAACAGAGCCAGGACTATATCAACCTCTTCTGCGCCAACATGATGGACT
  TGAACCGCAGAGCTGAGGCCATCGGATACGCCTACCCTACCCGGGACATCTTCATGGAAAACATCATGTT
  CTGTGGAATGGGCGGCTTCTCCGACTTCTACAAGCTCCGGTGGCTGGAGGCCATTCTCAGCTGGCAGAAA
  CAGCAGGAAGGATGCTTCGGGGAGCCTGATGCTGAAGATGAAGAATTATCTAAAGCTATTCAATATCAGC
  AGCATTTTTCGAGGAGAGTGAAGAGGCGAGAAAAACAATTTCCAGATGGCTGCTCCTCCCACAACACAGC
  CACAGCAGTGGCAGCCCTGGGTGGCTTCCTATACATCCTGGCAGAATACCCCCCAGCAAACAGAGAGCCA
  CACCCATCCACACCGCCACCACCAAGCAGCCGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218396 representing NM_001098514
  Red=Cloning site Green=Tags(s)
  
  MASLGLLLLLLLTALPPLWSSSLPGLDTAESKATIADLILSALERATVFLEQRLPEINLDGMVGVRVLEE
  QLKSVREKWAQEPLLQPLSLRVGMLGEKLEAAIQRSLHYLKLSDPKYLREFQLTLQPGFWKLPHAWIHTD
  ASLVYPTFGPQDSFSEERSDVCLVQLLGTGTDSSEPCGLSDLCRSLMTKPGCSGYCLSHQLLFFLWARMR
  GCTQGPLQQSQDYINLFCANMMDLNRRAEAIGYAYPTRDIFMENIMFCGMGGFSDFYKLRWLEAILSWQK
  QQEGCFGEPDAEDEELSKAIQYQQHFSRRVKRREKQFPDGCSSHNTATAVAALGGFLYILAEYPPANREP
  HPSTPPPPSSR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001098514
• ORF Size: 1083 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098514.3
• RefSeq Size: 1575 bp
• RefSeq ORF: 1086 bp
• Locus ID: 146556
• UniProt ID: Q6UX73
• Cytogenetics: 16p13.3
• Gene Summary: This gene is expressed predominantly in the thyroid. Based on expression patterns similar to thyroid transcription factors and proteins, this gene may function in the development and function of the thyroid. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]