H2AZ2 (NM_138635) Human Tagged ORF Clone
CAT#: RG217665
- TrueORF®
H2AFV (tGFP-tagged) - Human H2A histone family, member V (H2AFV), transcript variant 2
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_138635" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | H2AZ2 |
Synonyms | H2A.Z-2; H2AFV; H2AV |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG217665 representing NM_138635
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGAGGCAAAGCTGGAAAGGACAGTGGGAAGGCCAAGGCTAAGGCAGTATCTCGCTCACAGAGAG CTGGGCTACAGTTTCCTGTGGGCCGCATCCACAGACACTTGAAGACTCGCACCACAAGCCATGGAAGGGT GGGTGCCACTGCTGCCGTGTACAGTGCTGCGATTCTGGAGTACCTCACTGCAGAGGTGCTGGAGCTGGCA GGTAATGCTTCTAAGGATCTCAAAGTAAAGCGTATCACTCCGCGTCACTTGCAGCTTGCAATCCGTGGTG ATGAAGAGTTGGATTCTCTTATCAAGGCTACCATAGCTGGGGGTGAGAAGAGAAGGTGTTCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217665 representing NM_138635
Red=Cloning site Green=Tags(s) MAGGKAGKDSGKAKAKAVSRSQRAGLQFPVGRIHRHLKTRTTSHGRVGATAAVYSAAILEYLTAEVLELA GNASKDLKVKRITPRHLQLAIRGDEELDSLIKATIAGGEKRRCS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_138635 |
ORF Size | 342 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_138635.3 |
RefSeq Size | 825 bp |
RefSeq ORF | 345 bp |
Locus ID | 94239 |
UniProt ID | Q71UI9 |
Cytogenetics | 7p13 |
Domains | histone |
Protein Families | Druggable Genome |
Protein Pathways | Systemic lupus erythematosus |
Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. Several transcript variants encoding different isoforms, have been identified for this gene. [provided by RefSeq, Oct 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217665 | H2AFV (Myc-DDK-tagged)-Human H2A histone family, member V (H2AFV), transcript variant 2 |
USD 150.00 |
|
RC217665L3 | Lenti ORF clone of Human H2A histone family, member V (H2AFV), transcript variant 2, Myc-DDK-tagged |
USD 450.00 |
|
RC217665L4 | Lenti ORF clone of Human H2A histone family, member V (H2AFV), transcript variant 2, mGFP tagged |
USD 450.00 |
|
SC124291 | H2AFV (untagged)-Human H2A histone family, member V (H2AFV), transcript variant 2 |
USD 150.00 |
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