OR5AS1 (NM_001001921) Human Tagged ORF Clone

CAT#: RG217442

OR5AS1 (tGFP-tagged) - Human olfactory receptor, family 5, subfamily AS, member 1 (OR5AS1)

Product Images

Circular map for RG217442

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: OR5AS1
• Synonyms: OR11-168
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217442 representing NM_001001921
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGAGAGTAATTACACCATGCCAACTGAGTTCCTATTTGTTGGATTCACAGATTATCTACCTCTCA
  GAGTCACACTGTTCTTGGTATTCCTTCTGGTATATACATTAACTATGGTCGGAAATATACTCTTAATAAT
  TCTAGTTAATATTAATTCAAGCCTTCAAATTCCCATGTATTATTTTCTTAGCAACTTATCTTTCTTAGAC
  ATCAGCTGTTCTACAGCAATCACTCCTAAAATGCTGGCAAACTTCTTGGCATCCAGGAAAAGCATCTCTC
  CTTATGGGTGTGCACTACAAATGTTTTTCTTCGCTTCTTTTGCTGATGCTGAGTGCCTTATCCTGGCAGC
  AATGGCTTATGACCGCTATGCAGCCATCTGCAACCCACTGCTCTATACTACACTGATGTCTAGGAGAGTC
  TGTGTCTGCTTCATTGTGTTGGCATATTTCAGTGGAAGTACAACATCACTGGTCCATGTGTGCCTCACAT
  TCAGGCTGTCATTTTGTGGCTCCAATATCGTCAATCATTTTTTCTGTGATATCCCACCTCTTCTGGCTTT
  ATCATGTACAGACACTCAGATCAACCAGCTTCTGCTCTTTGCTTTGTGCAGCTTCATCCAGACCAGCACT
  TTTGTGGTAATATTTATTTCTTACTTCTGCATCCTCATCACTGTGTTGAGCATCAAGTCCTCAGGTGGCA
  GAAGCAAAACATTCTCCACTTGTGCTTCCCACCTCATAGCAGTCACCTTATTCTATGGAGCGCTCCTGTT
  TATGTACTTACAGCCCACCACTAGCTATTCCCTAGACACTGATAAGGTGGTGGCAGTGTTTTATACTGTT
  GTATTTCCCATGTTTAATCCAATAATTTATAGTTTCAGAAACAAGGATGTGAAAAATGCTCTCAAAAAGC
  TATTAGAAAGAATTGGATATTCAAATGAATGGTATTTAAATCGTTTAAGAATAGTCAATATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217442 representing NM_001001921
  Red=Cloning site Green=Tags(s)
  
  MLESNYTMPTEFLFVGFTDYLPLRVTLFLVFLLVYTLTMVGNILLIILVNINSSLQIPMYYFLSNLSFLD
  ISCSTAITPKMLANFLASRKSISPYGCALQMFFFASFADAECLILAAMAYDRYAAICNPLLYTTLMSRRV
  CVCFIVLAYFSGSTTSLVHVCLTFRLSFCGSNIVNHFFCDIPPLLALSCTDTQINQLLLFALCSFIQTST
  FVVIFISYFCILITVLSIKSSGGRSKTFSTCASHLIAVTLFYGALLFMYLQPTTSYSLDTDKVVAVFYTV
  VFPMFNPIIYSFRNKDVKNALKKLLERIGYSNEWYLNRLRIVNI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001921
• ORF Size: 972 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001921.1, NP_001001921.1
• RefSeq Size: 975 bp
• RefSeq ORF: 975 bp
• Locus ID: 219447
• UniProt ID: Q8N127
• Cytogenetics: 11q12.1
• Protein Families: Transmembrane
• Protein Pathways: Olfactory transduction
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]