STXBP5L (NM_014980) Human Tagged ORF Clone

CAT#: RG217266

STXBP5L (tGFP-tagged) - Human syntaxin binding protein 5-like (STXBP5L)

Product Images

Circular map for RG217266

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: STXBP5L
• Synonyms: LLGL4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217266 representing NM_014980
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAGTTTAATTTCCGAAAAGTTTTGGATGGCTTAACTGCCTCCTCCCCTGGCAGTGGTAGCAGCA
  GTGGCAGTAACAGTGGTGGTGGGGCTGGAAGTGGTTCCGTACATCCGGCGGGGACTGCAGGGGTTCTCAG
  AGAGGAAATTCAGGAAACTTTGACTTCGGAGTATTTCCAGATTTGCAAGACAGTTCGGCATGGTTTTCCT
  CATCAGCCCACAGCATTAGCCTTTGATCCAGTTCAGAAAATCTTGGCTATTGGGACGAGAACAGGTGCTA
  TACGAATACTCGGGAGACCTGGTGTTGATTGCTATTGCCAACATGAAAGTGGTGCAGCTGTCCTACAGCT
  CCAATTTTTGATCAATGAGGGTGCCTTGGTCAGTGCAAGTTCAGATGATACACTTCATTTGTGGAACCTT
  AGACAAAAAAGGCCAGCCATACTCCATTCTCTTAAATTTAACCGGGAACGAATTACTTACTGTCATCTAC
  CTTTCCAGAGTAAATGGCTTTATGTTGGAACAGAAAGAGGAAATACACATATTGTAAATATTGAATCTTT
  CATTCTTTCTGGATATGTTATCATGTGGAACAAGGCAATTGAACTATCCACTAAGACTCATCCAGGTCCA
  GTTGTACATTTAAGCGATAGCCCAAGAGATGAAGGCAAACTGCTAATAGGTTATGAAAATGGTACTGTAG
  TATTCTGGGACTTGAAATCTAAAAGAGCAGAACTGAGAGTTTATTATGATGAGGCTATTCATTCAATTGA
  TTGGCATCATGAGGGCAAACAGTTCATGTGCAGCCATTCAGATGGTAGTTTGACTTTATGGAACCTGAAA
  AGCCCAAGTCGCCCTTTCCAGACCACAATTCCACATGGAAAAAGTCAAAGAGAAGGAAGAAAATCTGAAT
  CTTGTAAACCAATTCTTAAAGTAGAATACAAGACCTGCAAAAACAGCGAACCATTCATAATATTCTCTGG
  TGGGCTGTCCTATGACAAAGCTTGTAGAAGACCAAGTTTAACCATCATGCATGGAAAAGCAATTACAGTA
  CTTGAAATGGATCATCCTATTGTTGAATTTCTAACTTTATGTGAAACGCCCTATCCAAATGAATTTCAAG
  AACCCTATGCTGTCGTGGTACTTCTGGAGAAAGATCTCATTGTAGTTGATCTGACACAAAGCAATTTTCC
  AATCTTTGAAAATCCATATCCCATGGACATTCATGAATCACCAGTTACATGCACAGCATACTTTGCAGAT
  TGTCCTCCGGATTTGATTCTAGTACTGTATTCTATAGGAGTCAAGCATAAAAAACAAGGATACAGTAATA
  AGGAGTGGCCAATCAGTGGAGGAGCTTGGAACCTTGGAGCACAAACATATCCAGAAATTATTATTACTGG
  TCATGCAGATGGATCAATAAAATTTTGGGATGCTTCTGCAATAACTCTGCAGATGCTGTACAAGCTAAAA
  ACTTCAAAAGTGTTTGAAAAACAGAAGGTAGGAGAAGGAAAACAAACATGTGAAATTGTAGAGGAAGACC
  CATTTGCCATTCAGATGATTTACTGGTGTCCAGAGAGCAGAATATTCTGTGTATCAGGAGTCTCTGCATA
  TGTCATAATTTATAAATTCAGCAGACATGAAATTACAACAGAAATAGTGTCATTAGAGGTACGACTTCAG
  TATGATGTTGAAGATATTATTACCCCTGAACCAGAAACAAGTCCTCCGTTTCCAGATCTCTCAGCCCAGC
  TTCCTTCTTCAAGGAGTCTTTCTGGGAGCACTAACACTGTTGCTAGTGAAGGAGTAACAAAGGACAGTAT
  TCCATGCCTCAATGTGAAGACACGGCCAGTGCGAATGCCTCCAGGATATCAAGCAGAACTTGTTATTCAA
  TTGGTGTGGGTAGATGGTGAACCTCCACAACAGATTACTAGTCTTGCTGTAAGCTCAGCATATGGAATAG
  TTGCATTTGGGAACTGCAATGGGTTGGCTGTGGTGGATTTTATACAGAAGACAGTACTGTTAAGCATGGG
  GACCATTGACCTATATAGATCAAGTGACTTATACCAGCGACAACCACGGTCTCCTCGAAAAAACAAACAG
  TTCATTGCAGACAACTTTTGCATGCGTGGCCTGTCTAACTTTTATCCTGATTTAACGAAACGGATCCGTA
  CTTCCTATCAGAGTTTAACTGAACTGAATGACAGTCCAGTTCCCCTAGAACTTGAGCGCTGCAAGTCTCC
  TACCTCAGACCATGTAAATGGACACTGCACAAGTCCAACTTCTCAGAGTTGCAGTTCTGGAAAACGTCTT
  TCTAGTGCCGATGTTTCAAAAGTAAATCGCTGGGGTCCTGGAAGACCACCATTTCGAAAGGCCCAGTCAG
  CAGCCTGCATGGAGATTTCTTTACCAGTTACAACAGAAGAAAACCGAGAAAATTCCTATAATCGTTCTAG
  AAGCTCTAGTATCTCCAGTATTGACAAAGATTCTAAAGAAGCAATTACAGCACTATACTTCATGGACTCC
  TTTGCACGGAAAAATGACTCTACCATCTCTCCTTGTCTGTTCGTTGGAACCAGTCTGGGAATGGTGTTAA
  TCATCTCCTTAAACCTACCATTAGCAGATGAACAAAGGTTTACAGAGCCAGTCATGGTATTGCCAAGTGG
  TACATTCCTCTCATTGAAAGGAGCTGTGCTAACATTCTCCTGTATGGACCGAATGGGTGGATTAATGCAA
  CCGCCATATGAAGTTTGGAGGGATCCAAACAACATAGATGAAAATGAAAAATCTTGGAGAAGGAAAGTGG
  TAATGAACTCATCTTCTGCATCCCAAGAAATAGGAGATCATCAGTATACAATAATCTGCTCAGAAAAACA
  AGCCAAAGTCTTCTCACTGCCTTCTCAGACTTGCCTTTATGTTCATAACATCACGGAGACATCTTTTATA
  CTGCAAGCAAATGTGGTGGTCATGTGTAGCAGTGCCTGCTTGGCATGCTTTTGTGCTAACGGACATATCA
  TGATAATGAGCCTACCTAGTCTTCGCCCAATGTTGGATGTTAATTATTTGCCACTGACAGACATGAGGAT
  AGCACGAACATTTTGTTTTACCAATGAAGGACAGGCATTATACCTCGTCTCTCCTACTGAAATTCAGCGG
  TTAACATACAGCCAAGAAATGTGTGATAATCTACAGGACATGCTAGGAGATTTGTTTACTCCCATAGAGA
  CACCAGAAGCTCAAAACAGAGGCTTTCTCAAGGGACTGTTTGGTGGAAGCGGACAAACATTTGACAGAGA
  AGAGCTCTTTGGGGAAGCTTCGGCAGGAAAAGCATCCCGCAGCCTTGCGCAACACATTCCTGGACCAGGT
  AGTATAGAAGGGATGAAAGGCGCTGCTGGAGGGGTGATGGGAGAGCTGACCCGTGCACGGATTGCACTTG
  ATGAAAGAGGACAGAGGCTAGGAGAGCTGGAAGAGAAAACTGCAGGCATGATGACCAGTGCAGAAGCATT
  TTCCAAACATGCACATGAGTTAATGCTGAAATACAAGGATAAGAAATGGTACCAATTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217266 representing NM_014980
  Red=Cloning site Green=Tags(s)
  
  MKKFNFRKVLDGLTASSPGSGSSSGSNSGGGAGSGSVHPAGTAGVLREEIQETLTSEYFQICKTVRHGFP
  HQPTALAFDPVQKILAIGTRTGAIRILGRPGVDCYCQHESGAAVLQLQFLINEGALVSASSDDTLHLWNL
  RQKRPAILHSLKFNRERITYCHLPFQSKWLYVGTERGNTHIVNIESFILSGYVIMWNKAIELSTKTHPGP
  VVHLSDSPRDEGKLLIGYENGTVVFWDLKSKRAELRVYYDEAIHSIDWHHEGKQFMCSHSDGSLTLWNLK
  SPSRPFQTTIPHGKSQREGRKSESCKPILKVEYKTCKNSEPFIIFSGGLSYDKACRRPSLTIMHGKAITV
  LEMDHPIVEFLTLCETPYPNEFQEPYAVVVLLEKDLIVVDLTQSNFPIFENPYPMDIHESPVTCTAYFAD
  CPPDLILVLYSIGVKHKKQGYSNKEWPISGGAWNLGAQTYPEIIITGHADGSIKFWDASAITLQMLYKLK
  TSKVFEKQKVGEGKQTCEIVEEDPFAIQMIYWCPESRIFCVSGVSAYVIIYKFSRHEITTEIVSLEVRLQ
  YDVEDIITPEPETSPPFPDLSAQLPSSRSLSGSTNTVASEGVTKDSIPCLNVKTRPVRMPPGYQAELVIQ
  LVWVDGEPPQQITSLAVSSAYGIVAFGNCNGLAVVDFIQKTVLLSMGTIDLYRSSDLYQRQPRSPRKNKQ
  FIADNFCMRGLSNFYPDLTKRIRTSYQSLTELNDSPVPLELERCKSPTSDHVNGHCTSPTSQSCSSGKRL
  SSADVSKVNRWGPGRPPFRKAQSAACMEISLPVTTEENRENSYNRSRSSSISSIDKDSKEAITALYFMDS
  FARKNDSTISPCLFVGTSLGMVLIISLNLPLADEQRFTEPVMVLPSGTFLSLKGAVLTFSCMDRMGGLMQ
  PPYEVWRDPNNIDENEKSWRRKVVMNSSSASQEIGDHQYTIICSEKQAKVFSLPSQTCLYVHNITETSFI
  LQANVVVMCSSACLACFCANGHIMIMSLPSLRPMLDVNYLPLTDMRIARTFCFTNEGQALYLVSPTEIQR
  LTYSQEMCDNLQDMLGDLFTPIETPEAQNRGFLKGLFGGSGQTFDREELFGEASAGKASRSLAQHIPGPG
  SIEGMKGAAGGVMGELTRARIALDERGQRLGELEEKTAGMMTSAEAFSKHAHELMLKYKDKKWYQF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014980
• ORF Size: 3558 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014980.3
• RefSeq Size: 4536 bp
• RefSeq ORF: 3561 bp
• Locus ID: 9515
• UniProt ID: Q9Y2K9
• Cytogenetics: 3q13.33
• Gene Summary: The protein encoded by this gene is similar to syntaxin-binding protein 5 and contains ten N-terminal WD40 repeats, four variable region WD40 repeats, and a C-terminal R-SNARE domain. Studies of the orthologous proteins in mouse and rat have shown that the encoded protein may inhibit exocytosis in neurosecretory cells, and may negatively regulate the secretion of insulin. A missense variant in this gene is likely the cause of an infantile-onset neurodegenerative disorder diagnosed in two siblings of consanguineous parents. [provided by RefSeq, Jan 2017]