SLC2A14 (NM_153449) Human Tagged ORF Clone

CAT#: RG216671

SLC2A14 (tGFP-tagged) - Human solute carrier family 2 (facilitated glucose transporter), member 14 (SLC2A14)

Product Images

Circular map for RG216671

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SLC2A14
• Synonyms: GLUT14; SLC2A3P3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216671 representing NM_153449
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTTCACAATGGTGGCCATGTGTCTGGAATTGGTGGGTTCTTGGTCTCACTGACTTCAAGAATGA
  AGCCGCACACCCTCGCAGTCACCCCAGCTCTGATCTTTGCCATCACAGTTGCTACAATCGGCTCTTTCCA
  GTTTGGCTACAACACTGGGGTCATCAATGCTCCTGAGACGATCATAAAGGAATTTATCAATAAAACTTTG
  ACGGACAAGGCAAATGCCCCTCCCTCTGAGGTGCTGCTCACGAATCTCTGGTCCTTGTCTGTGGCCATAT
  TTTCCGTCGGGGGTATGATCGGCTCCTTTTCCGTCGGACTCTTTGTTAACCGCTTTGGCAGGCGCAATTC
  AATGCTGATTGTCAACCTGTTGGCTGCCACTGGTGGCTGCCTTATGGGACTGTGTAAAATAGCTGAGTCA
  GTTGAAATGCTGATCCTGGGCCGCTTGGTTATTGGCCTCTTCTGCGGACTCTGCACAGGTTTTGTGCCCA
  TGTACATTGGAGAGATCTCGCCTACTGCCCTGAGGGGTGCCTTTGGCACTCTCAACCAGCTGGGCATAGT
  TATTGGAATTCTGGTGGCCCAGATCTTTGGTCTGGAACTCATCCTTGGGTCTGAAGAGCTATGGCCGGTG
  CTATTAGGCTTTACCATCCTTCCAGCTATCCTGCAAAGTGCAGCCCTTCCATGTTGCCCTGAAAGTCCCA
  GATTTTTGCTCATTAACAGAAAAAAAGAGGAGAATGCTACGCGGATCCTCCAGCGGTTGTGGGGCACCCA
  GGATGTATCCCAAGACATCCAGGAGATGAAAGATGAGAGTGCAAGGATGTCACAAGAAAAGCAAGTCACC
  GTGCTGGAGCTCTTTAGAGTGTCCAGCTACCGACAGCCCATCATCATTTCCATTGTGCTCCAGCTCTCTC
  AGCAGCTCTCTGGGATCAATGCTGTGTTCTATTACTCAACAGGAATCTTCAAGGATGCAGGTGTTCAACA
  GCCCATCTATGCCACCATCAGCGCGGGTGTGGTTAATACTATCTTCACTTTACTTTCTCTATTTCTGGTG
  GAAAGGGCAGGAAGAAGGACTCTGCATATGATAGGCCTTGGAGGGATGGCTTTTTGTTCCACGCTCATGA
  CTGTTTCTTTGTTATTAAAGAATCACTATAATGGGATGAGCTTTGTCTGTATTGGGGCTATCTTGGTCTT
  TGTGGCCTGTTTTGAAATTGGACCAGGCCCCATTCCCTGGTTTATTGTGGCCGAACTCTTCAGCCAGGGC
  CCCCGCCCAGCTGCGATGGCAGTGGCCGGCTGCTCCAACTGGACCTCCAACTTCCTAGTCGGATTGCTCT
  TCCCCTCTGCTGCTTACTATTTAGGAGCCTACGTTTTTATTATCTTCACCGGCTTCCTCATTACCTTCTT
  GGCCTTTACCTTCTTCAAAGTCCCTGAGACCCGTGGCAGGACTTTTGAGGATATCACACGGGCCTTTGAA
  GGGCAGGCACACGGTGCAGATAGATCTGGGAAGGACGGCGTCATGGGGATGAACAGCATCGAGCCTGCTA
  AGGAGACCACCACCAATGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216671 representing NM_153449
  Red=Cloning site Green=Tags(s)
  
  MEFHNGGHVSGIGGFLVSLTSRMKPHTLAVTPALIFAITVATIGSFQFGYNTGVINAPETIIKEFINKTL
  TDKANAPPSEVLLTNLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIVNLLAATGGCLMGLCKIAES
  VEMLILGRLVIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVIGILVAQIFGLELILGSEELWPV
  LLGFTILPAILQSAALPCCPESPRFLLINRKKEENATRILQRLWGTQDVSQDIQEMKDESARMSQEKQVT
  VLELFRVSSYRQPIIISIVLQLSQQLSGINAVFYYSTGIFKDAGVQQPIYATISAGVVNTIFTLLSLFLV
  ERAGRRTLHMIGLGGMAFCSTLMTVSLLLKNHYNGMSFVCIGAILVFVACFEIGPGPIPWFIVAELFSQG
  PRPAAMAVAGCSNWTSNFLVGLLFPSAAYYLGAYVFIIFTGFLITFLAFTFFKVPETRGRTFEDITRAFE
  GQAHGADRSGKDGVMGMNSIEPAKETTTNV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153449
• ORF Size: 1560 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153449.4
• RefSeq Size: 2195 bp
• RefSeq ORF: 1563 bp
• Locus ID: 144195
• UniProt ID: Q8TDB8
• Cytogenetics: 12p13.31
• Protein Families: Transmembrane
• Gene Summary: Members of the glucose transporter (GLUT) family, including SLC2A14, are highly conserved integral membrane proteins that transport hexoses such as glucose and fructose into all mammalian cells. GLUTs show tissue and cell-type specific expression (Wu and Freeze, 2002 [PubMed 12504846]).[supplied by OMIM, Mar 2008]