Folate Receptor 4 (IZUMO1R) (NM_001080486) Human Tagged ORF Clone

CAT#: RG216623

FOLR4 (tGFP-tagged) - Human folate receptor 4 (delta) homolog (mouse) (FOLR4)

Product Images

Circular map for RG216623

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Folate Receptor 4
• Synonyms: folate receptor 4 (delta) homolog; folate receptor 4 (delta) homolog (mouse); Folbp3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216623 representing NM_001080486
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATGCTGGTGGCCGCTCCTGCTAGAGCTGTGGACAGTCATGCCCACCTGGGCTGGGGACGAGCTGC
  TCAACATCTGCATGAATGCCAAACACCACAAGAGAGTGCCCAGCCCAGAAGACAAGCTCTATGAGGAGTG
  CATCCCCTGGAAGGACAATGCCTGCTGCACCCTCACGACAAGCTGGGAAGCCCATCTGGATGTATCCCCA
  CTCTACAACTTCAGCCTGTTTCACTGTGGACTGCTGATGCCTGGCTGTCGGAAGCACTTCATCCAGGCTA
  TCTGCTTCTATGAGTGCTCCCCAAACCTGGGGCCCTGGATCCAGCCAGTGGCCCCGAGTGGGCAGGGAGA
  GCGAGTTGTGAATGTGCCGCTGTGCCAGGAGGACTGTGAGGAGTGGTGGGAAGACTGTCGCATGTCTTAC
  ACATGCAAATCCAACTGGCGTGGTGGCTGGGACTGGAGTCAGGGGAAGAACCGCTGCCCCAAAGGGGCCC
  AGTGCCTCCCTTTCTCCCATTACTTCCCCACCCCAGCTGACCTGTGTGAGAAGACTTGGAGCAATTCCTT
  CAAAGCCAGCCCTGAGCGACGGAACAGTGGGCGGTGTCTCCAGAAGTGGTTTGAGCCTGCTCAGGGCAAC
  CCCAATGTGGCCGTGGCCCGCCTCTTCGCCAGCTCTGCCCCATCCTGGGAACTGTCCTACACCATCATGG
  TCTGCTCCCTGTTCCTGCCGTTCCTTTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216623 representing NM_001080486
  Red=Cloning site Green=Tags(s)
  
  MACWWPLLLELWTVMPTWAGDELLNICMNAKHHKRVPSPEDKLYEECIPWKDNACCTLTTSWEAHLDVSP
  LYNFSLFHCGLLMPGCRKHFIQAICFYECSPNLGPWIQPVAPSGQGERVVNVPLCQEDCEEWWEDCRMSY
  TCKSNWRGGWDWSQGKNRCPKGAQCLPFSHYFPTPADLCEKTWSNSFKASPERRNSGRCLQKWFEPAQGN
  PNVAVARLFASSAPSWELSYTIMVCSLFLPFLS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080486
• ORF Size: 728 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080486.1, NP_001073955.1
• RefSeq Size: 732 bp
• RefSeq ORF: 731 bp
• Locus ID: 390243
• Cytogenetics: 11q21
• Gene Summary: Receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for species-specific gamete recognition and fertilization. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Does not bind folate.[UniProtKB/Swiss-Prot Function]