OR8B4 (NM_001005196) Human Tagged ORF Clone

CAT#: RG216381

OR8B4 (tGFP-tagged) - Human olfactory receptor, family 8, subfamily B, member 4 (OR8B4)

Product Images

Circular map for RG216381

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: OR8B4
• Synonyms: OR8B4P; OR11-315
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216381 representing NM_001005196
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCTGAGAAACAGCTCCTCAGTGACTGAGTTTATCCTTGTGGGATTATCAGAACAGCCAGAGCTCC
  AGCTCCCTCTTTTCCTTCTATTCTTAGGGATCTATGTGTTCACTGTGGTGGGCAACTTGGGCTTGATCAC
  CTTAATTGGGATAAATCCTAGCCTTCACACCCCCATGTACTTTTTCCTCTTCAACTTGTCCTTTATAGAT
  CTCTGTTATTCCTGTGTGTTTACCCCCAAAATGCTGAATGACTTTGTTTCAGAAAGTATCATCTCTTATG
  TGGGATGTATGACTCAGCTATTTTTCTTCTGTTTCTTTGTCAATTCTGAGTGCTATGTGTTGGTATCAAT
  GGCCTATGATCGCTATGTGGCCATCTGCAACCCCCTGCTCTACATGGTCACCATGTCCCCAAGGGTCTGC
  TTTCTGCTGATGTTTGGTTCCTATGTGGTAGGGTTTGCTGGGGCCATGGCCCACACTGGAAGCATGCTGC
  GACTGACCTTCTGTGATTCCAACGTCATTGACCATTATCTGTGTGACGTTCTCCCCCTCTTGCAGCTCTC
  CTGCACCAGCACCCATGTCAGTGAGCTGGTATTTTTCATTGTTGTTGGAGTAATCACCATGCTATCCAGC
  ATAAGCATCGTCATCTCTTACGCTTTGATACTCTCCAACATCCTCTGTATTCCTTCTGCAGAGGGCAGAT
  CCAAAGCCTTTAGCACATGGGGCTCCCACATAATTGCTGTTGCTCTGTTTTTTGGGTCAGGGACATTCAC
  CTACTTAACAACATCTTTTCCTGGCTCTATGAACCATGGCAGATTTGCCTCAGTCTTTTACACCAATGTG
  GTTCCCATGCTTAACCCTTCGATCTACAGTTTGAGGAATAAGGATGATAAACTTGCCCTGGGCAAAACCC
  TGAAGAGAGTGCTCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216381 representing NM_001005196
  Red=Cloning site Green=Tags(s)
  
  MTLRNSSSVTEFILVGLSEQPELQLPLFLLFLGIYVFTVVGNLGLITLIGINPSLHTPMYFFLFNLSFID
  LCYSCVFTPKMLNDFVSESIISYVGCMTQLFFFCFFVNSECYVLVSMAYDRYVAICNPLLYMVTMSPRVC
  FLLMFGSYVVGFAGAMAHTGSMLRLTFCDSNVIDHYLCDVLPLLQLSCTSTHVSELVFFIVVGVITMLSS
  ISIVISYALILSNILCIPSAEGRSKAFSTWGSHIIAVALFFGSGTFTYLTTSFPGSMNHGRFASVFYTNV
  VPMLNPSIYSLRNKDDKLALGKTLKRVLF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005196
• ORF Size: 927 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005196.1, NP_001005196.1
• RefSeq Size: 930 bp
• RefSeq ORF: 930 bp
• Locus ID: 283162
• UniProt ID: Q96RC9
• Cytogenetics: 11q24.2
• Protein Families: Transmembrane
• Protein Pathways: Olfactory transduction
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. This olfactory receptor gene is a segregating pseudogene, where some individuals have an allele that encodes a functional olfactory receptor, while other individuals have an allele encoding a protein that is predicted to be non-functional. [provided by RefSeq, Jul 2015]