NRG2 (NM_013982) Human Tagged ORF Clone

CAT#: RG216128

NRG2 (tGFP-tagged) - Human neuregulin 2 (NRG2), transcript variant 3

Product Images

Circular map for RG216128

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NRG2
• Synonyms: DON1; HRG2; NTAK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216128 representing NM_013982
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCAGGTTTGCTGCTCAGCGCTGCCGCCGCCGCCACTGGAGAAGGGTCGGTGCAGCAGCTACAGCG
  ACAGCAGCAGCAGCAGCAGCGAGAGGAGCAGCAGCAGCAGCAGCAGCAGCAGCGAGAGCGGCAGCAGCAG
  CAGGAGCAGCAGCAACAACAGCAGCATCTCTCGTCCCGCTGCGCCCCCAGAGCCGCGGCCGCAGCAACAG
  CCGCAGCCCCGCAGCCCCGCAGCCCGGAGAGCCGCCGCCCGTTCGCGAGCCGCAGCCGCCGGCGGCATGA
  GGCGCGACCCGGCCCCCGGCTTCTCCATGCTGCTCTTCGGTGTGTCGCTCGCCTGCTACTCGCCCAGCCT
  CAAGTCAGTGCAGGACCAGGCGTACAAGGCACCCGTGGTGGTGGAGGGCAAGGTACAGGGGCTGGTCCCA
  GCCGGCGGCTCCAGCTCCAACAGCACCCGAGAGCCGCCCGCCTCGGGTCGGGTGGCGTTGGTAAAGGTGC
  TGGACAAGTGGCCGCTCCGGAGCGGGGGGCTGCAGCGCGAGCAGGTGATCAGCGTGGGCTCCTGTGTGCC
  GCTCGAAAGGAACCAGCGCTACATCTTTTTCCTGGAGCCCACGGAACAGCCCTTAGTCTTTAAGACGGCC
  TTTGCCCCCCTCGATACCAACGGCAAAAATCTCAAGAAAGAGGTGGGCAAGATCCTGTGCACTGACTGCG
  CCACCCGGCCCAAGTTGAAGAAGATGAAGAGCCAGACGGGACAGGTGGGTGAGAAGCAATCGCTGAAGTG
  TGAGGCAGCAGCCGGTAATCCCCAGCCTTCCTACCGTTGGTTCAAGGATGGCAAGGAGCTCAACCGCAGC
  CGAGACATTCGCATCAAATATGGCAACGGCAGAAAGAACTCACGACTACAGTTCAACAAGGTGAAGGTGG
  AGGACGCTGGGGAGTATGTCTGCGAGGCCGAGAACATCCTGGGGAAGGACACCGTCCGGGGCCGGCTTTA
  CGTCAACAGCGTGAGCACCACCCTGTCATCCTGGTCGGGGCACGCCCGGAAGTGCAACGAGACAGCCAAG
  TCCTATTGCGTCAATGGAGGCGTCTGCTACTACATCGAGGGCATCAACCAGCTCTCCTGCAAATGTCCAA
  ATGGATTCTTCGGACAGAGATGTTTGGAGAAACTGCCTTTGCGATTGTACATGCCAGATCCTAAGCAAAA
  GCACCTTGGATTTGAATTAAAGGAAGCCGAGGAGCTGTACCAGAAGAGGGTCCTGACCATCACGGGCATC
  TGCGTGGCTCTGCTGGTCGTGGGCATCGTCTGTGTGGTGGCCTACTGCAAGACCAAAAAACAGCGGAAGC
  AGATGCACAACCACCTCCGGCAGAACATGTGCCCGGCCCATCAGAACCGGAGCTTGGCCAATGGGCCCAG
  CCACCCCCGGCTGGACCCAGAGGAGATCCAGATGGCAGATTATATTTCCAAGAACGTGCCAGCCACAGAC
  CATGTCATCAGGAGAGAAACTGAGACCACCTTCTCTGGGAGCCACTCCTGTTCTCCTTCTCACCACTGCT
  CCACAGCCACACCCACCTCCAGCCACAGACACGAGAGCCACACGTGGAGCCTGGAACGTTCTGAGAGCCT
  GACTTCTGACTCCCAGTCGGGGATCATGCTATCATCAGTGGGTACCAGCAAATGCAACAGCCCAGCATGT
  GTGGAGGCCCGGGCAAGGCGGGCAGCAGCCTACAACCTGGAGGAGCGGCGCAGGGCCACCGCGCCACCCT
  ATCACGATTCCGTGGACTCCCTTCGCGACTCCCCACACAGCGAGAGGTACGTGTCGGCCCTGACCACGCC
  CGCGCGCCTCTCGCCCGTGGACTTCCACTACTCGCTGGCCACGCAGGTGCCAACTTTCGAGATCACGTCC
  CCCAACTCGGCGCACGCCGTGTCGCTGCCGCCGGCGGCGCCCATCAGTTACCGCCTGGCCGAGCAGCAGC
  CGTTACTGCGGCACCCGGCGCCCCCCGGCCCGGGACCCGGACCCGGGCCCGGGCCCGGGCCCGGCGCAGA
  CATGCAGCGCAGCTATGACAGCTACTATTACCCCGCGGCGGGGCCCGG[AT]CCGCGGCGCGGGACCTGC
  GCGCTCGGCGGCAGCCTGGGCAGCCTGCCTGCCAGCCCCTTCCGCATCCCCGAGGACGACGAGTACGAGA
  CCACGCAGGAGTGCGCGCCCCCGCCGCCGCCGCGGCCGCGCGCGCGCGGTGCGTCCCGCAGGACGTCGGC
  GGGGCCCCGGCGCTGGCGCCGCTCGCGCCTCAACGGGCTGGCGGCGCAGCGCGCACGGGCGGCGAGGGAC
  TCGCTGTCGCTGAGCAGCGGCTCGGGCGGCGGCTCAGCCTCGGCGTCGGACGACGACGCGGACGACGCGG
  ACGGGGCGCTGGCGGCCGAGAGCACACCTTTCCTGGGCCTGCGTGGGGCGCACGACGCGCTGCGCTCGGA
  CTCGCCGCCACTGTGCCCGGCGGCCGACAGCAGGACTTACTACTCACTGGACAGCCACAGCACGCGGGCC
  AGCAGCAGACACAGCCGCGGGCCGCCCCCGCGGGCCAAGCAGGACTCGGCGCCACTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216128 representing NM_013982
  Red=Cloning site Green=Tags(s)
  
  MRQVCCSALPPPPLEKGRCSSYSDSSSSSSERSSSSSSSSSESGSSSRSSSNNSSISRPAAPPEPRPQQQ
  PQPRSPAARRAAARSRAAAAGGMRRDPAPGFSMLLFGVSLACYSPSLKSVQDQAYKAPVVVEGKVQGLVP
  AGGSSSNSTREPPASGRVALVKVLDKWPLRSGGLQREQVISVGSCVPLERNQRYIFFLEPTEQPLVFKTA
  FAPLDTNGKNLKKEVGKILCTDCATRPKLKKMKSQTGQVGEKQSLKCEAAAGNPQPSYRWFKDGKELNRS
  RDIRIKYGNGRKNSRLQFNKVKVEDAGEYVCEAENILGKDTVRGRLYVNSVSTTLSSWSGHARKCNETAK
  SYCVNGGVCYYIEGINQLSCKCPNGFFGQRCLEKLPLRLYMPDPKQKHLGFELKEAEELYQKRVLTITGI
  CVALLVVGIVCVVAYCKTKKQRKQMHNHLRQNMCPAHQNRSLANGPSHPRLDPEEIQMADYISKNVPATD
  HVIRRETETTFSGSHSCSPSHHCSTATPTSSHRHESHTWSLERSESLTSDSQSGIMLSSVGTSKCNSPAC
  VEARARRAAAYNLEERRRATAPPYHDSVDSLRDSPHSERYVSALTTPARLSPVDFHYSLATQVPTFEITS
  PNSAHAVSLPPAAPISYRLAEQQPLLRHPAPPGPGPGPGPGPGPGADMQRSYDSYYYPAAGPXXPRRGTC
  ALGGSLGSLPASPFRIPEDDEYETTQECAPPPPPRPRARGASRRTSAGPRRWRRSRLNGLAAQRARAARD
  SLSLSSGSGGGSASASDDDADDADGALAAESTPFLGLRGAHDALRSDSPPLCPAADSRTYYSLDSHSTRA
  SSRHSRGPPPRAKQDSAPL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013982
• ORF Size: 2577 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013982.2, NP_053585.1
• RefSeq Size: 3963 bp
• RefSeq ORF: 2577 bp
• Locus ID: 9542
• UniProt ID: O14511
• Cytogenetics: 5q31.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ErbB signaling pathway
• Gene Summary: This gene encodes a novel member of the neuregulin family of growth and differentiation factors. Through interaction with the ERBB family of receptors, this protein induces the growth and differentiation of epithelial, neuronal, glial, and other types of cells. The gene consists of 12 exons and the genomic structure is similar to that of neuregulin 1, another member of the neuregulin family of ligands. The products of these genes mediate distinct biological processes by acting at different sites in tissues and eliciting different biological responses in cells. This gene is located close to the region for demyelinating Charcot-Marie-Tooth disease locus, but is not responsible for this disease. Alternative transcript variants encoding distinct isoforms have been described. [provided by RefSeq, May 2010]