PHF8 (NM_015107) Human Tagged ORF Clone
CAT#: RG214763
- TrueORF®
PHF8 (tGFP-tagged) - Human PHD finger protein 8 (PHF8), transcript variant 2
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_015107" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | PHF8 |
Synonyms | JHDM1F; KDM7B; MRXSSD; ZNF422 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG214763 representing NM_015107
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCGGTGCCGGTGTATTGCCTCTGCCGGCTGCCTTACGATGTGACCCGCTTCATGATCGAGTGTG ACATGTGCCAGGACTGGTTTCATGGCAGTTGTGTTGGTGTTGAAGAGGAGAAGGCTGCTGACATTGACCT CTACCACTGCCCCAACTGTGAAGTCTTGCATGGGCCCTCCATTATGAAAAAACGCCGTGGATCTTCAAAG GGGCATGATACACACAAGGGGAAACCAGTGAAGACCGGGAGCCCTACGTTCGTCAGAGAGCTCCGGAGTA GGACTTTTGACAGCTCAGATGAAGTGATTCTGAAGCCCACTGGAAATCAACTGACCGTGGAATTCCTGGA AGAAAATAGCTTCAGTGTGCCCATCCTGGTCCTGAAGAAGGATGGGTTGGGCATGACGCTGCCCTCGCCA TCATTCACTGTGAGGGATGTTGAACACTATGTTGGTTCTGACAAAGAGATTGATGTGATTGATGTGACCC GCCAGGCTGACTGCAAGATGAAGCTTGGTGATTTTGTGAAATACTATTACAGCGGGAAGAGGGAGAAAGT CCTCAATGTCATTAGTTTGGAATTCTCTGATACCAGACTTTCTAACCTTGTGGAGACACCGAAGATTGTT CGAAAGCTGTCATGGGTCGAAAACTTGTGGCCAGAGGAATGTGTCTTTGAGAGACCCAATGTACAGAAGT ACTGCCTCATGAGTGTGCGAGATAGCTATACAGACTTTCACATTGACTTTGGTGGCACCTCTGTCTGGTA CCATGTACTCAAGGGTGAAAAGATCTTCTACCTGATCCGCCCAACAAATGCCAATCTGACTCTCTTTGAG TGCTGGAGCAGTTCCTCTAATCAGAATGAGATGTTCTTTGGGGACCAGGTGGACAAGTGCTACAAGTGTT CCGTGAAGCAAGGACAGACACTTTTCATTCCCACAGGGTGGATCCATGCTGTGCTGACGCCTGTGGACTG CCTTGCCTTTGGAGGGAACTTCTTACACAGCCTTAACATCGAGATGCAGCTCAAAGCCTATGAGATTGAG AAGCGGCTGAGCACAGCAGACCTCTTCAGATTCCCCAACTTTGAGACCATCTGTTGGTATGTGGGAAAGC ACATCCTGGACATCTTTCGCGGTTTGCGAGAGAACAGGAGACACCCTGCCTCCTACCTGGTCCATGGTGG CAAAGCCTTGAACTTGGCCTTTAGAGCCTGGACAAGGAAAGAAGCTCTGCCAGACCATGAGGATGAGATC CCGGAGACAGTGCGAACCGTACAGCTCATTAAAGATCTGGCCAGGGAGATCCGCCTGGTGGAAGACATCT TCCAACAGAACGTTGGGAAGACGAGCAATATCTTTGGGCTGCAGAGGATCTTCCCAGCCGGCTCCATTCC CCTAACCAGGCCAGCCCATTCCACTTCAGTGTCCATGTCCAGGCTGTCACTGCCCTCCAAAAATGGTTCA AAGAAGAAAGGCCTGAAGCCCAAGGAACTCTTCAAGAAGGCAGAGCGAAAGGGCAAGGAGAGTTCAGCCT TGGGGCCTGCTGGCCAGTTGAGCTATAATCTCATGGACACATACAGTCATCAGGCACTGAAGACAGGCTC TTTCCAGAAAGCAAAGTTCAACATCACTGGTGCCTGCTTGAATGACTCAGATGACGACTCACCAGACTTG GACCTTGATGGAAATGAGAGCCCATTGGCCCTATTGATGTCTAACGGCAGTACGAAAAGGGTGAAGAGTT TATCCAAATCTCGGCGAACCAAGATAGCAAAGAAGGTAGACAAGGCTAGGCTGATGGCAGAACAGGTGAT GGAAGACGAATTTGACTTGGATTCAGATGATGAGCTGCAGATTGACGAGAGATTGGGAAAGGAGAAGGCG ACCCTGATAATAAGACCAAAATTTCCCCGGAAATTGCCCCGTGCGAAGCCTTGCTCTGACCCCAACCGAG TTCGTGAACCAGGAGAAGTTGAGTTTGACATTGAGGAGGACTATACAACAGATGAGGACATGGTGGAAGG GGTTGAAGGCAAGCTTGGGAATGGTAGTGGCGCTGGTGGCATTCTTGATCTGCTCAAGGCCAGCAGGCAG GTGGGGGGACCTGACTATGCTGCCCTCACCGAGGCCCCAGCTTCTCCCAGCACTCAGGAGGCCATCCAGG GCATGCTGTGCATGGCCAACCTGCAGTCCTCATCGTCCTCACCGGCTACCTCTAGCCTGCAGGCCTGGTG GACTGGGGGACAGGATCGAAGCAGTGGGAGCTCCAGCAGTGGGCTGGGCACAGTGTCTAACAGTCCTGCT TCCCAGCGCACCCCAGGGAAGCGGCCCATCAAGCGGCCAGCATACTGGAGAACCGAGAGCGAGGAGGAGG AGGAGAACGCCAGTCTGGATGAACAGGACAGCTTGGGAGCGTGCTTCAAGGATGCAGAGTATATCTATCC TTCACTGGAGTCTGATGATGATGACCCTGCTTTGAAATCTCGACCCAAGAAAAAGAAGAATTCAGATGAT GCTCCATGGAGTCCTAAAGCCCGCGTGACCCCAACTCTGCCGAAGCAGGACCGTCCTGTGCGTGAGGGGA CCCGGGTAGCCTCTATTGAGACAGGTTTGGCTGCAGCAGCTGCAAAGCTGGCCCAGCAGGAGCTACAGAA GGCCCAAAAGAAGAAATATATCAAGAAGAAGCCTTTGCTGAAGGAGGTAGAACAGCCTCGCCCTCAAGAC TCCAATCTCAGTCTGACAGTACCAGCCCCCACTGTGGCTGCCACACCACAACTTGTCACCTCCTCCTCAC CCCTGCCTCCTCCTGAGCCTAAACAAGAGGCCCTGTCAGGAAGTCTCGCTGACCATGAGTACACCGCTCG TCCCAATGCCTTTGGCATGGCCCAGGCAAACCGCAGCACCACACCTATGGCCCCCGGTGTCTTCTTGACC CAGCGGCGCCCTTCAGTTGGCTCCCAGAGCAATCAGGCAGGACAAGGAAAGCGTCCCAAAAAGGGCCTGG CCACAGCAAAGCAGAGACTCGGCCGTATCCTGAAAATCCACAGAAATGGCAAACTACTTCTG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214763 representing NM_015107
Red=Cloning site Green=Tags(s) MASVPVYCLCRLPYDVTRFMIECDMCQDWFHGSCVGVEEEKAADIDLYHCPNCEVLHGPSIMKKRRGSSK GHDTHKGKPVKTGSPTFVRELRSRTFDSSDEVILKPTGNQLTVEFLEENSFSVPILVLKKDGLGMTLPSP SFTVRDVEHYVGSDKEIDVIDVTRQADCKMKLGDFVKYYYSGKREKVLNVISLEFSDTRLSNLVETPKIV RKLSWVENLWPEECVFERPNVQKYCLMSVRDSYTDFHIDFGGTSVWYHVLKGEKIFYLIRPTNANLTLFE CWSSSSNQNEMFFGDQVDKCYKCSVKQGQTLFIPTGWIHAVLTPVDCLAFGGNFLHSLNIEMQLKAYEIE KRLSTADLFRFPNFETICWYVGKHILDIFRGLRENRRHPASYLVHGGKALNLAFRAWTRKEALPDHEDEI PETVRTVQLIKDLAREIRLVEDIFQQNVGKTSNIFGLQRIFPAGSIPLTRPAHSTSVSMSRLSLPSKNGS KKKGLKPKELFKKAERKGKESSALGPAGQLSYNLMDTYSHQALKTGSFQKAKFNITGACLNDSDDDSPDL DLDGNESPLALLMSNGSTKRVKSLSKSRRTKIAKKVDKARLMAEQVMEDEFDLDSDDELQIDERLGKEKA TLIIRPKFPRKLPRAKPCSDPNRVREPGEVEFDIEEDYTTDEDMVEGVEGKLGNGSGAGGILDLLKASRQ VGGPDYAALTEAPASPSTQEAIQGMLCMANLQSSSSSPATSSLQAWWTGGQDRSSGSSSSGLGTVSNSPA SQRTPGKRPIKRPAYWRTESEEEEENASLDEQDSLGACFKDAEYIYPSLESDDDDPALKSRPKKKKNSDD APWSPKARVTPTLPKQDRPVREGTRVASIETGLAAAAAKLAQQELQKAQKKKYIKKKPLLKEVEQPRPQD SNLSLTVPAPTVAATPQLVTSSSPLPPPEPKQEALSGSLADHEYTARPNAFGMAQANRSTTPMAPGVFLT QRRPSVGSQSNQAGQGKRPKKGLATAKQRLGRILKIHRNGKLLL SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015107 |
ORF Size | 3072 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015107.3 |
RefSeq Size | 5776 bp |
RefSeq ORF | 3075 bp |
Locus ID | 23133 |
UniProt ID | Q9UPP1 |
Cytogenetics | Xp11.22 |
Protein Families | Druggable Genome, Transcription Factors |
Gene Summary | The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. The protein has an N-terminal PHD finger and a central Jumonji C domain. This gene is thought to function as a transcription activator. Defects in this gene are a cause of syndromic X-linked Siderius type intellectual disability (MRXSSD) and over-expression of this gene is associated with several forms of cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214763 | PHF8 (Myc-DDK-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 2 |
USD 891.00 |
|
RC214763L1 | Lenti-ORF clone of PHF8 (Myc-DDK-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 2 |
USD 1,191.00 |
|
RC214763L2 | Lenti-ORF clone of PHF8 (mGFP-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 2 |
USD 1,191.00 |
|
RC214763L3 | Lenti-ORF clone of PHF8 (Myc-DDK-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 2 |
USD 1,191.00 |
|
RC214763L4 | Lenti-ORF clone of PHF8 (mGFP-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 2 |
USD 1,191.00 |
|
SC107370 | PHF8 (untagged)-Human PHD finger protein 8 (PHF8), transcript variant 2 |
USD 892.00 |
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