CROP (LUC7L3) (NM_016424) Human Tagged ORF Clone

CAT#: RG214406

LUC7L3 (tGFP-tagged) - Human LUC7-like 3 (S. cerevisiae) (LUC7L3), transcript variant 1

Product Images

Circular map for RG214406

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CROP
• Synonyms: CRA; CREAP-1; CROP; hLuc7A; LUC7A; OA48-18
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214406 representing NM_016424
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTTCGGCCGCGCAGTTGTTGGATGAGTTAATGGGCCGGGACCGAAACCTAGCCCCGGACGAGAAGC
  GCAGCAACGTGCGGTGGGACCACGAGAGCGTTTGTAAATATTATCTCTGTGGTTTTTGTCCTGCGGAATT
  GTTCACAAATACACGTTCTGATCTTGGTCCGTGTGAAAAAATTCATGATGAAAATCTACGAAAACAGTAT
  GAGAAGAGCTCTCGTTTCATGAAAGTTGGCTATGAGAGAGATTTTTTGCGATACTTACAGAGCTTACTTG
  CAGAAGTAGAACGTAGGATCAGACGAGGCCATGCTCGTTTGGCATTATCTCAAAACCAGCAGTCTTCTGG
  GGCCGCTGGCCCAACAGGCAAAAATGAAGAAAAAATTCAGGTTCTAACAGACAAAATTGATGTACTTCTG
  CAACAGATTGAAGAATTAGGGTCTGAAGGAAAAGTAGAAGAAGCCCAGGGGATGATGAAATTAGTTGAGC
  AATTAAAAGAAGAGAGAGAACTGCTAAGGTCCACAACGTCGACAATTGAAAGCTTTGCTGCACAAGAAAA
  ACAAATGGAAGTTTGTGAAGTATGTGGAGCCTTTTTAATAGTAGGAGATGCCCAGTCCCGGGTAGATGAC
  CATTTGATGGGAAAACAACACATGGGCTATGCCAAAATTAAAGCTACTGTAGAAGAATTAAAAGAAAAGT
  TAAGGAAAAGAACCGAAGAACCTGATCGTGATGAGCGTCTAAAAAAGGAGAAGCAAGAAAGAGAAGAAAG
  AGAAAAAGAACGGGAGAGAGAAAGGGAAGAAAGAGAAAGGAAAAGACGAAGGGAAGAGGAAGAAAGAGAA
  AAAGAAAGGGCTCGTGACAGAGAAAGAAGAAAGAGAAGTCGTTCACGAAGTAGACACTCAAGCCGAACAT
  CAGACAGAAGATGCAGCAGGTCTCGGGACCACAAAAGGTCACGAAGTAGAGAAAGAAGGCGGAGCAGAAG
  TAGAGATCGACGAAGAAGCAGAAGCCATGATCGATCAGAAAGAAAACACAGATCTCGAAGTCGGGATCGA
  AGAAGATCAAAAAGCCGGGATCGAAAGTCATATAAGCACAGGAGCAAAAGTCGGGACAGAGAACAAGATA
  GAAAATCCAAGGAGAAAGAAAAGAGGGGATCTGATGATAAAAAAAGTAGTGTGAAGTCCGGTAGTCGAGA
  AAAGCAGAGTGAAGACACAAACACTGAATCGAAGGAAAGTGATACTAAGAATGAGGTCAATGGGACCAGT
  GAAGACATTAAATCTGAAGGTGACACTCAGTCCAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214406 representing NM_016424
  Red=Cloning site Green=Tags(s)
  
  MISAAQLLDELMGRDRNLAPDEKRSNVRWDHESVCKYYLCGFCPAELFTNTRSDLGPCEKIHDENLRKQY
  EKSSRFMKVGYERDFLRYLQSLLAEVERRIRRGHARLALSQNQQSSGAAGPTGKNEEKIQVLTDKIDVLL
  QQIEELGSEGKVEEAQGMMKLVEQLKEERELLRSTTSTIESFAAQEKQMEVCEVCGAFLIVGDAQSRVDD
  HLMGKQHMGYAKIKATVEELKEKLRKRTEEPDRDERLKKEKQEREEREKEREREREERERKRRREEEERE
  KERARDRERRKRSRSRSRHSSRTSDRRCSRSRDHKRSRSRERRRSRSRDRRRSRSHDRSERKHRSRSRDR
  RRSKSRDRKSYKHRSKSRDREQDRKSKEKEKRGSDDKKSSVKSGSREKQSEDTNTESKESDTKNEVNGTS
  EDIKSEGDTQSN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016424
• ORF Size: 1296 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016424.5
• RefSeq Size: 3477 bp
• RefSeq ORF: 1299 bp
• Locus ID: 51747
• UniProt ID: O95232
• Cytogenetics: 17q21.33
• Domains: DUF259
• Protein Families: Stem cell - Pluripotency
• Gene Summary: This gene encodes a protein with an N-terminal half that contains cysteine/histidine motifs and leucine zipper-like repeats, and the C-terminal half is rich in arginine and glutamate residues (RE domain) and arginine and serine residues (RS domain). This protein localizes with a speckled pattern in the nucleus, and could be involved in the formation of splicesome via the RE and RS domains. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2009]