LONRF3 (NM_001031855) Human Tagged ORF Clone

CAT#: RG214236

LONRF3 (tGFP-tagged) - Human LON peptidase N-terminal domain and ring finger 3 (LONRF3), transcript variant 1

Product Images

Circular map for RG214236

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LONRF3
• Synonyms: RNF127
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214236 representing NM_001031855
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCAGTACGGATCGAACAGATGCTGAGCTTGCCCGCCGAGGTCAGCAGCGACAACTTGGAGTCGG
  CGGAGCGAGGGGCATCAGCGGCCCAAGTAGACATGGGCCCCCACCCAAAGGTGGCTGCAGAGGGCCCCGC
  ACCTCTACCGACGCGGGAGCCAGAGCAAGAGCAGTCTCCGGGGACCTCAACGCCGGAGAGCAAAGTCCTG
  CTCACGCAGGCAGACGCCTTGGCGTCCCGGGGGCGAATCCGTGAAGCCCTCGAGGTGTATAGACAGCTCT
  CCGAGCGGCAGCAGCTGGTGGCTGAGCAGCTGGAGCAGCTGGTGCGCTGCCTGGCGGAGAAAGTCCCGCA
  AGGCGAGGCGCTGGCGCCGGCGCCCCCGGACGAGGGTAGCACTGCAAGCGGCACCGTGGCGGCGGAAGAG
  ACGGGGGCCGCCGCGGCTGCGGCGGCCACCGAGGTGTGGGACGGCTTTAAGTGCCGGAAATGTCATGGGT
  TTCTATCAGACCCCGTGTCCTTGTCGTGTGGCCACACCTTTTGTAAACTGTGCCTGGAACGTGGGCGGGC
  CGCCGACCGGCGCTGTGCGCTGTGCGGGGTCAAGCTCTCCGCCTTGATGGTGGCCACTGGGCGGGCGCGT
  GGAGCCCGGCGGGCTGGGCAGCAGCCGCCGCCGCCGCTGCGAGTCAACGTGGTGCTCAGCGGCCTCCTCG
  GCAAGTTGTTTCCAGGCCCAGCGCGAGCGTCGCAACTCCGGCACGAGGGCAACCGACTGTACCGCGAGCG
  CCAGGTGGAGGCGGCACTGCTCAAGTACAACGAGGCAGTTAAGTTGGCTCCAAATGACCACTTGCTTTAT
  AGCAATCGGTCTCAGATTTATTTCACCTTGGAGTCTCATGAGAATGCACTGCATGATGCAGAAATAGCAT
  GTAAGCTCCGCCCGATGGGTTTTAAGGCACATTTCAGAAAAGCCCAAGCCTTAGCCACCCTAGGCAAGGT
  GGAGGAGGCACTAAGGGAGTTTCTCTACTGTGTATCCCTTGATGGAAAGAACAAGAGAGCAAGATGTGAA
  GCCCAAAGAGACAATCTGGAGCTCCCACATTGTTCTAGTCAGGAGGAAGCAGCAGCCAGGGGAGATGGCA
  GCAGTCTGATGGACCCAGCTAAAGTGAAGGGGGATGGTCAGCAGCACCACATGAAAGACCAGGAAGAAGA
  GGAGGAGAAGTGGGATGCTACCTCTCCAAAAGCTGCTTCCAGCAAGACTGGAAAATGCCAGGAAAAGAAA
  AGGAAACATTGCCAGATTGAATCCCAAGAAGAAACGGGGATGCCTAATAAAGCCTCCAAGCAAGATCCTC
  CCACTGATCAGGGGGACAAACCTGCTCTCAGTTTACCACTTGCATCTTTCGACGCATCTGACCTTGAATG
  CGCTCTATGTATGAGATTATTCTATGAGCCAGTCACAACACCTTGCGGGCATACTTTTTGCTTAAAATGC
  CTAGAAAGATGCCTAGATCACAACGCAAAGTGTCCATTGTGCAAAGACGGTCTTTCACAGTGCTTGGCAT
  CAAGAAAATACAGCAAAAATGTAATAATGGAGGAGCTCATAGCTAAATTCCTTCCAGAAGAACTGAAGGA
  ACGAAGGAAGCTTTATGAAGAGGAAATGGAAGAACTTTCTAACCTTAATAAGAATGTGCCTATTTTCGTG
  TGTACTATGGCCTATCCCACCGTTCCTTGTCCCCTGCACATCTTTGAGCCTTGTTACCGCCTGATGATTC
  GTAGATGCATTGAGACAGGCACGAGACAGTTTGGCATGTGCCTTGGAGATCCTGTCAAAGGGTTTGCAGA
  ATATGGCTGCATCCTAGAGATCAGAAATGTTCAATTCCTTGCTGATGGCCGCTCAGTGGTTGACAGCATA
  GGCAAGAGGCGCTTCAGGGTGCTCCATCAGAGCCAGCGGGATGGCTACAACACAGCCGACATTGAATACA
  TTGAAGACCAAAAGGTTCAGGGAGAGGATTGTGCTGAGCTCATGGGATTACATAACTGTGTCTATCAGCA
  AGCATCATTGTGGTTTCATTCGCTCAAATTATCCCTAAAGAATCGGATACTCAATCACTTTGGTCCCATG
  CCGGAGAAAGACGCCGATCCTCAGATGAACCCGAATGGCCCAGCCTGGTGCTGGTGGATGTTAGCAGTTC
  TTCCCTTGGAAAGCCGAGCTCAGCTCCCCTTCCTAGCAATGAGGTCCTTAAAGGACAGACTGAATGGTAT
  TCGACGAGTCCTGGCCTTCATATCCCGAAACCAAAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214236 representing NM_001031855
  Red=Cloning site Green=Tags(s)
  
  MESVRIEQMLSLPAEVSSDNLESAERGASAAQVDMGPHPKVAAEGPAPLPTREPEQEQSPGTSTPESKVL
  LTQADALASRGRIREALEVYRQLSERQQLVAEQLEQLVRCLAEKVPQGEALAPAPPDEGSTASGTVAAEE
  TGAAAAAAATEVWDGFKCRKCHGFLSDPVSLSCGHTFCKLCLERGRAADRRCALCGVKLSALMVATGRAR
  GARRAGQQPPPPLRVNVVLSGLLGKLFPGPARASQLRHEGNRLYRERQVEAALLKYNEAVKLAPNDHLLY
  SNRSQIYFTLESHENALHDAEIACKLRPMGFKAHFRKAQALATLGKVEEALREFLYCVSLDGKNKRARCE
  AQRDNLELPHCSSQEEAAARGDGSSLMDPAKVKGDGQQHHMKDQEEEEEKWDATSPKAASSKTGKCQEKK
  RKHCQIESQEETGMPNKASKQDPPTDQGDKPALSLPLASFDASDLECALCMRLFYEPVTTPCGHTFCLKC
  LERCLDHNAKCPLCKDGLSQCLASRKYSKNVIMEELIAKFLPEELKERRKLYEEEMEELSNLNKNVPIFV
  CTMAYPTVPCPLHIFEPCYRLMIRRCIETGTRQFGMCLGDPVKGFAEYGCILEIRNVQFLADGRSVVDSI
  GKRRFRVLHQSQRDGYNTADIEYIEDQKVQGEDCAELMGLHNCVYQQASLWFHSLKLSLKNRILNHFGPM
  PEKDADPQMNPNGPAWCWWMLAVLPLESRAQLPFLAMRSLKDRLNGIRRVLAFISRNQN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001031855
• ORF Size: 2277 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031855.1, NP_001027026.1
• RefSeq Size: 2625 bp
• RefSeq ORF: 2280 bp
• Locus ID: 79836
• UniProt ID: Q496Y0
• Cytogenetics: Xq24
• Protein Families: Druggable Genome, Protease
• Gene Summary: The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. Multiple alternatively spliced transcript variants have been suggested, but their full length natures are not clear. [provided by RefSeq, Jul 2008]