TUT7 (NM_024617) Human Tagged ORF Clone

CAT#: RG214200

ZCCHC6 (tGFP-tagged) - Human zinc finger, CCHC domain containing 6 (ZCCHC6), transcript variant 1

Product Images

Circular map for RG214200

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TUT7
• Synonyms: PAPD6; TENT3B; ZCCHC6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214200 representing NM_024617
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGATACAGCAAAACCTTATTTCGTGAAGCGCACTAAAGACCGGGGGACTATGGATGATGATGACT
  TCAGAAGGGGTCACCCCCAACAAGATTATTTAATAATAGATGACCATGCTAAAGGCCATGGCAGTAAAAT
  GGAAAAGGGCCTTCAAAAAAAGAAGATAACACCAGGGAACTATGGGAATACCCCCAGAAAAGGACCATGT
  GCTGTTTCCAGCAATCCATATGCATTTAAAAACCCAATCTACAGTCAACCCGCTTGGATGAATGACAGCC
  ACAAAGATCAGAGTAAGAGATGGCTGTCTGATGAACATACTGGTAATTCAGACAACTGGAGAGAATTCAA
  ACCTGGACCTAGAATTCCTGTTATAAACCGACAAAGAAAAGACTCCTTTCAAGAAAATGAAGATGGTTAT
  AGGTGGCAAGACACAAGAGGCTGCAGAACTGTAAGACGACTGTTTCATAAAGACCTAACAAGCCTAGAAA
  CCACGTCAGAAATGGAAGCAGGAAGTCCTGAAAACAAGAAGCAGAGGTCCAGACCTAGGAAGCCACGGAA
  GACTAGAAATGAGGAAAATGAGCAGGATGGAGACTTGGAAGGCCCTGTGATCGATGAGTCTGTACTTTCA
  ACGAAGGAGCTGCTAGGCTTACAGCAGGCTGAGGAGAGACTGAAGAGAGACTGCATTGACAGGCTAAAAA
  GGCGACCACGAAACTACCCTACAGCAAAGTACACCTGCAGACTCTGTGATGTTTTAATTGAATCCATTGC
  ATTTGCCCATAAGCATATCAAGGAAAAGAGGCACAAGAAAAACATTAAGGAGAAGCAAGAGGAAGAGTTG
  CTCACTACGTTACCCCCACCAACACCCTCCCAGATAAATGCAGTTGGCATTGCCATTGACAAAGTGGTAC
  AGGAATTTGGCTTACACAATGAGAACTTGGAACAGAGGCTGGAAATTAAACGTATCATGGAAAATGTGTT
  CCAACACAAGTTACCAGATTGTTCCCTAAGATTATATGGGTCATCCTGTAGCAGATTGGGTTTCAAAAAT
  TCGGATGTAAACATTGACATCCAGTTTCCAGCCATTATGTCTCAGCCAGATGTCCTCTTACTTGTTCAAG
  AATGTTTAAAGAACAGTGACTCCTTTATTGATGTTGATGCAGACTTCCATGCTAGGGTGCCAGTGGTGGT
  GTGCAGAGAAAAGCAAAGTGGTCTTCTGTGTAAAGTGAGCGCAGGAAATGAAAATGCTTGTCTGACAACA
  AAGCATTTAACTGCCCTTGGAAAACTAGAACCAAAGCTGGTTCCTTTGGTGATTGCATTTAGGTACTGGG
  CAAAGCTTTGCAGTATAGATCGCCCTGAAGAAGGAGGTCTGCCACCTTATGTGTTTGCCCTGATGGCCAT
  TTTCTTTCTTCAGCAGAGGAAAGAACCCCTTTTGCCTGTATATCTAGGATCATGGATTGAAGGATTCTCA
  TTAAGCAAACTAGGGAATTTCAACCTTCAAGACATTGAAAAAGATGTTGTGATCTGGGAACATACTGACA
  GTGCTGCAGGGGACACAGGCATAACAAAAGAAGAGGCACCAAGAGAAACGCCGATTAAAAGGGGACAGGT
  GTCATTAATATTGGATGTGAAACACCAGCCTTCAGTACCAGTTGGGCAGCTCTGGGTGGAATTGCTGCGG
  TTCTATGCTTTAGAATTTAATTTGGCTGATTTAGTGATAAGTATTCGTGTCAAAGAATTGGTATCTCGGG
  AATTGAAGGATTGGCCCAAAAAGCGCATTGCCATTGAAGATCCCTACTCTGTTAAAAGAAATGTGGCAAG
  AACCCTAAATAGTCAACCTGTGTTTGAATATATACTTCATTGTTTAAGGACAACATACAAGTATTTTGCT
  CTTCCACACAAAATTACAAAATCCAGCCTTCTAAAGCCTCTGAATGCAATTACATGTATTTCAGAACATT
  CTAAAGAAGTAATAAATCATCATCCAGATGTACAAACAAAAGATGATAAGCTCAAAAACTCAGTTTTGGC
  CCAAGGTCCTGGTGCTACCAGTTCAGCTGCAAATACCTGTAAGGTACAGCCACTTACTCTTAAAGAGACT
  GCTGAAAGTTTTGGAAGCCCACCAAAAGAAGAAATGGGAAATGAACACATCAGTGTCCACCCTGAAAACT
  CAGACTGTATCCAAGCAGATGTTAACTCTGATGATTACAAGGGTGATAAAGTATACCATCCAGAAACAGG
  AAGGAAAAACGAGAAAGAGAAAGTTGGAAGGAAGGGCAAGCATCTGTTGACTGTTGATCAGAAACGTGGA
  GAGCATGTTGTCTGTGGCAGCACACGTAATAATGAGTCAGAGAGCACTTTGGATTTAGAAGGCTTCCAAA
  ATCCCACAGCTAAAGAGTGTGAGGGACTTGCCACTTTAGATAACAAGGCTGATCTTGATGGAGAAAGTAC
  AGAAGGTACTGAGGAACTAGAAGACTCTCTAAACCACTTTACCCACTCAGTACAGGGCCAGACATCAGAA
  ATGATTCCCTCTGATGAAGAGGAGGAGGACGACGAAGAAGAGGAGGAGGAAGAAGAACCTAGGCTCACCA
  TTAACCAAAGGGAAGATGAAGATGGCATGGCTAATGAAGATGAGTTAGACAACACCTACACTGGGTCAGG
  GGATGAGGACGCCCTATCTGAAGAGGATGATGAGTTAGGCGAAGCTGCTAAGTATGAAGACGTGAAAGAA
  TGTGGAAAACATGTAGAAAGAGCTCTCCTAGTGGAACTTAATAAAATAAGTCTCAAGGAAGAAAATGTAT
  GTGAAGAAAAAAATTCACCTGTGGATCAGTCTGATTTTTTTTATGAATTCAGTAAACTTATCTTCACCAA
  AGGCAAGTCTCCTACGGTAGTGTGCAGCTTATGCAAACGAGAGGGTCATCTAAAGAAGGACTGTCCTGAA
  GACTTCAAAAGAATCCAGCTAGAACCTCTGCCACCATTAACACCCAAGTTTTTAAATATCTTAGATCAAG
  TCTGTATCCAGTGTTATAAGGATTTTTCTCCAACAATTATAGAAGATCAGGCTCGTGAACATATTCGGCA
  AAACCTAGAAAGTTTCATAAGACAGGACTTTCCAGGAACTAAATTGAGCCTGTTTGGCTCCTCCAAAAAT
  GGATTTGGGTTCAAACAGAGTGACCTTGACGTCTGTATGACAATTAATGGACTTGAAACTGCTGAGGGAT
  TGGACTGTGTCAGAACTATTGAAGAATTAGCAAGAGTCCTCAGAAAACATTCAGGTCTGAGAAACATCTT
  ACCTATTACAACAGCAAAGGTGCCAATTGTGAAGTTCTTCCATTTGAGAAGTGGTCTGGAAGTAGATATC
  AGTTTGTATAACACATTGGCCCTTCATAACACAAGGCTTTTATCTGCTTATTCCGCCATTGATCCCAGAG
  TGAAGTATTTGTGCTATACCATGAAAGTATTTACAAAGATGTGTGATATTGGTGATGCATCTAGAGGCAG
  CTTATCATCGTATGCATATACTCTTATGGTGCTATATTTTCTCCAGCAGAGGAATCCACCAGTCATTCCT
  GTCCTTCAAGAGATATACAAAGGTGAAAAGAAACCTGAAATATTTGTTGATGGCTGGAATATTTATTTTT
  TTGATCAAATAGATGAACTGCCTACCTATTGGTCAGAATGTGGAAAAAATACAGAATCTGTTGGGCAGTT
  ATGGTTGGGCCTTCTTCGTTTCTACACAGAGGAATTTGATTTTAAAGAACATGTTATTAGCATCAGGAGA
  AAAAGTCTGCTTACAACTTTTAAGAAACAGTGGACCTCAAAATACATTGTTATTGAAGATCCCTTTGATT
  TGAATCATAATCTTGGAGCTGGATTATCAAGGAAAATGACAAATTTTATAATGAAGGCTTTTATCAATGG
  TAGAAGAGTATTTGGTATTCCTGTCAAGGGATTTCCAAAGGACTACCCCTCAAAAATGGAATACTTTTTT
  GATCCAGATGTGTTAACTGAAGGAGAGCTGGCCCCAAATGATAGATGTTGTCGAATTTGTGGAAAAATCG
  GACACTTCATGAAGGACTGTCCTATGAGGAGAAAAGTAAGACGGCGGCGAGATCAGGAAGATGCCCTGAA
  CCAAAGATACCCTGAGAACAAGGAAAAAAGAAGCAAAGAGGACAAAGAAATTCACAACAAGTACACAGAA
  AGGGAGGTGTCAACAAAAGAAGATAAGCCCATACAGTGCACACCTCAGAAAGCCAAGCCAATGCGGGCAG
  CTGCTGACCTGGGGAGGGAGAAGATCCTCAGGCCACCAGTAGAAAAATGGAAGAGACAGGATGACAAAGA
  CTTAAGAGAAAAACGTTGTTTTATTTGTGGAAGAGAAGGGCACATTAAAAAGGAATGCCCACAGTTTAAA
  GGCTCTTCAGGTAGCCTTTCCAGTAAATATATGACTCAGGGAAAAGCCTCAGCGAAGAGGACCCAGCAGG
  AATCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214200 representing NM_024617
  Red=Cloning site Green=Tags(s)
  
  MGDTAKPYFVKRTKDRGTMDDDDFRRGHPQQDYLIIDDHAKGHGSKMEKGLQKKKITPGNYGNTPRKGPC
  AVSSNPYAFKNPIYSQPAWMNDSHKDQSKRWLSDEHTGNSDNWREFKPGPRIPVINRQRKDSFQENEDGY
  RWQDTRGCRTVRRLFHKDLTSLETTSEMEAGSPENKKQRSRPRKPRKTRNEENEQDGDLEGPVIDESVLS
  TKELLGLQQAEERLKRDCIDRLKRRPRNYPTAKYTCRLCDVLIESIAFAHKHIKEKRHKKNIKEKQEEEL
  LTTLPPPTPSQINAVGIAIDKVVQEFGLHNENLEQRLEIKRIMENVFQHKLPDCSLRLYGSSCSRLGFKN
  SDVNIDIQFPAIMSQPDVLLLVQECLKNSDSFIDVDADFHARVPVVVCREKQSGLLCKVSAGNENACLTT
  KHLTALGKLEPKLVPLVIAFRYWAKLCSIDRPEEGGLPPYVFALMAIFFLQQRKEPLLPVYLGSWIEGFS
  LSKLGNFNLQDIEKDVVIWEHTDSAAGDTGITKEEAPRETPIKRGQVSLILDVKHQPSVPVGQLWVELLR
  FYALEFNLADLVISIRVKELVSRELKDWPKKRIAIEDPYSVKRNVARTLNSQPVFEYILHCLRTTYKYFA
  LPHKITKSSLLKPLNAITCISEHSKEVINHHPDVQTKDDKLKNSVLAQGPGATSSAANTCKVQPLTLKET
  AESFGSPPKEEMGNEHISVHPENSDCIQADVNSDDYKGDKVYHPETGRKNEKEKVGRKGKHLLTVDQKRG
  EHVVCGSTRNNESESTLDLEGFQNPTAKECEGLATLDNKADLDGESTEGTEELEDSLNHFTHSVQGQTSE
  MIPSDEEEEDDEEEEEEEEPRLTINQREDEDGMANEDELDNTYTGSGDEDALSEEDDELGEAAKYEDVKE
  CGKHVERALLVELNKISLKEENVCEEKNSPVDQSDFFYEFSKLIFTKGKSPTVVCSLCKREGHLKKDCPE
  DFKRIQLEPLPPLTPKFLNILDQVCIQCYKDFSPTIIEDQAREHIRQNLESFIRQDFPGTKLSLFGSSKN
  GFGFKQSDLDVCMTINGLETAEGLDCVRTIEELARVLRKHSGLRNILPITTAKVPIVKFFHLRSGLEVDI
  SLYNTLALHNTRLLSAYSAIDPRVKYLCYTMKVFTKMCDIGDASRGSLSSYAYTLMVLYFLQQRNPPVIP
  VLQEIYKGEKKPEIFVDGWNIYFFDQIDELPTYWSECGKNTESVGQLWLGLLRFYTEEFDFKEHVISIRR
  KSLLTTFKKQWTSKYIVIEDPFDLNHNLGAGLSRKMTNFIMKAFINGRRVFGIPVKGFPKDYPSKMEYFF
  DPDVLTEGELAPNDRCCRICGKIGHFMKDCPMRRKVRRRRDQEDALNQRYPENKEKRSKEDKEIHNKYTE
  REVSTKEDKPIQCTPQKAKPMRAAADLGREKILRPPVEKWKRQDDKDLREKRCFICGREGHIKKECPQFK
  GSSGSLSSKYMTQGKASAKRTQQES
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024617
• ORF Size: 4485 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024617.4
• RefSeq Size: 5648 bp
• RefSeq ORF: 4488 bp
• Locus ID: 79670
• UniProt ID: Q5VYS8
• Cytogenetics: 9q21.33
• Domains: zf-CCHC, PAP_assoc
• Gene Summary: Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:19703396, PubMed:25480299). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (By similarity). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets (PubMed:25480299). Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (PubMed:25979828). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 uridylation is strongly enhanced in the presence of LIN28A (PubMed:22898984). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed:25979828, PubMed:28671666). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (PubMed:25979828). Does not play a role in replication-dependent histone mRNA degradation (PubMed:18172165). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed:25979828, PubMed:25480299, PubMed:19703396, PubMed:22898984, PubMed:18172165, PubMed:28671666). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (PubMed:30122351).[UniProtKB/Swiss-Prot Function]