PTPRU (NM_133177) Human Tagged ORF Clone

CAT#: RG214198

PTPRU (tGFP-tagged) - Human protein tyrosine phosphatase, receptor type, U (PTPRU), transcript variant 2

Product Images

Circular map for RG214198

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTPRU
• Synonyms: FMI; hPTP-J; PCP-2; PTP; PTP-J; PTP-PI; PTP-RO; PTPPSI; PTPRO; PTPU2; R-PTP-PSI; R-PTP-U
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214198 representing NM_133177
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGTGCCCAGGCGCTCGTGCTGGCACTCACCTTCCAGCTCTGCGCGCCGGAGACCGAGACTCCGG
  CAGCTGGCTGCACCTTCGAGGAGGCAAGTGACCCAGCAGTGCCCTGCGAGTACAGCCAGGCCCAGTACGA
  TGACTTCCAGTGGGAGCAAGTGCGAATCCACCCTGGCACCCGGGCACCTGCGGACCTGCCCCACGGCTCC
  TACTTGATGGTCAACACTTCCCAGCATGCCCCAGGCCAGCGAGCCCATGTCATCTTCCAGAGCCTGAGCG
  AGAATGATACCCACTGTGTGCAGTTCAGCTACTTCCTGTACAGCCGGGACGGGCACAGCCCGGGCACCCT
  GGGCGTCTACGTGCGCGTTAATGGGGGCCCCCTGGGCAGTGCTGTGTGGAATATGACTGGATCCCACGGC
  CGTCAGTGGCACCAGGCTGAGCTGGCTGTCAGCACTTTCTGGCCCAATGAATATCAGGTGCTGTTTGAGG
  CCCTCATCTCCCCAGACCGCAGGGGCTACATGGGCCTAGATGACATCCTGCTTCTCAGCTACCCCTGCGC
  AAAGGCCCCACACTTCTCCCGCCTGGGCGACGTGGAGGTCAACGCGGGCCAGAACGCGTCGTTCCAGTGC
  ATGGCCGCGGGCAGAGCGGCCGAGGCCGAACGCTTCCTCTTGCAACGGCAGAGCGGGGCGCTGGTGCCGG
  CGGCGGGCGTGCGGCACATCAGCCACCGGCGCTTCCTGGCCACTTTCCCGCTGGCTGCCGTGAGCCGCGC
  CGAGCAGGACCTGTACCGCTGTGTGTCCCAGGCCCCGCGCGGCGCGGGCGTCTCTAACTTCGCGGAGCTC
  ATCGTCAAGGAGCCCCCAACTCCCATCGCGCCCCCACAGCTGCTGCGTGCTGGCCCCACCTACCTCATCA
  TCCAGCTCAACACCAACTCCATCATTGGCGACGGGCCGATCGTGCGCAAGGAGATTGAGTACCGCATGGC
  GCGCGGGCCCTGGGCTGAGGTGCACGCCGTCAGCCTGCAGACCTACAAGCTGTGGCACCTCGACCCCGAC
  ACAGAGTATGAGATCAGCGTGCTGCTCACGCGTCCCGGAGACGGCGGCACTGGCCGCCCTGGGCCACCCC
  TCATCAGCCGCACCAAATGCGCAGAGCCCATGAGGGCCCCCAAAGGCCTGGCTTTTGCTGAGATCCAGGC
  CCGTCAGCTGACCCTGCAGTGGGAACCACTGGGCTACAACGTGACGCGTTGCCACACCTATACTGTGTCG
  CTGTGCTATCACTACACCCTGGGCAGCAGCCACAACCAGACCATCCGAGAGTGTGTGAAGACAGAGCAAG
  GTGTCAGCCGCTACACCATCAAGAACCTGCTGCCCTATCGGAACGTTCACGTGAGGCTTGTCCTCACTAA
  CCCTGAGGGGCGCAAAGAGGGCAAGGAGGTCACTTTCCAGACGGATGAGGATGTGCCCAGTGGGATTGCA
  GCCGAGTCCCTGACCTTCACTCCACTGGAGGACATGATCTTCCTCAAGTGGGAGGAGCCCCAGGAGCCCA
  ATGGTCTCATCACCCAGTATGAGATCAGCTACCAGAGCATCGAGTCATCAGACCCGGCAGTGAACGTGCC
  AGGCCCACGACGTACCATCTCCAAGCTCCGCAATGAGACCTACCATGTCTTCTCCAACCTGCACCCAGGC
  ACCACCTACCTGTTCTCCGTGCGGGCCCGCACAGGCAAAGGCTTCGGCCAGGCGGCACTCACTGAGATAA
  CCACTAACATCTCTGCTCCCAGCTTTGATTATGCCGACATGCCGTCACCCCTGGGCGAGTCTGAGAACAC
  CATCACCGTGCTGCTGAGGCCGGCACAGGGCCGCGGTGCGCCCATCAGTGTGTACCAGGTGATTGTGGAG
  GAGGAGCGGGCGCGGAGGCTGCGGCGGGAGCCAGGTGGACAGGACTGCTTCCCAGTGCCATTGACCTTCG
  AGGCGGCGCTGGCCCGAGGCCTGGTGCACTACTTCGGGGCCGAACTGGCGGCCAGCAGTCTACCTGAGGC
  CATGCCCTTTACCGTGGGTGACAACCAGACCTACCGAGGCTTCTGGAACCCACCACTTGAGCCTAGGAAG
  GCCTATCTCATCTACTTCCAGGCAGCAAGCCACCTGAAGGGGGAGACCCGGCTGAATTGCATCCGCATTG
  CCAGGAAAGCTGCCTGCAAGGAAAGCAAGCGGCCCCTGGAGGTGTCCCAGAGATCGGAGGAGATGGGGCT
  TATCCTGGGCATCTGTGCAGGGGGGCTTGCTGTCCTCATCCTTCTCCTGGGTGCCATCATTGTCATCATC
  CGCAAAGGGAAGCCGGTGAACATGACCAAGGCCACCGTCAACTACCGCCAGGAGAAGACACACATGATGA
  GCGCCGTGGACCGCAGCTTCACAGACCAGAGCACCCTGCAGGAGGACGAGCGGCTGGGCCTGTCCTTCAT
  GGACACCCATGGCTACAGCACCCGGGGAGACCAGCGCAGCGGTGGGGTCACTGAGGCCAGCAGCCTCCTG
  GGGGGCTCCCCGAGGCGTCCCTGTGGCCGGAAGGGCTCCCCATACCACACGGGGCAGCTGCACCCTGCGG
  TGCGTGTCGCAGACCTTCTGCAGCACATCAACCAGATGAAGACGGCCGAGGGTTACGGCTTCAAGCAGGA
  GTATGAGAGCTTCTTTGAAGGCTGGGACGCCACAAAGAAGAAAGACAAGGTCAAGGGCAGCCGGCAGGAG
  CCAATGCCTGCCTATGATCGGCACCGAGTGAAACTGCACCCGATGCTGGGAGACCCCAATGCCGACTACA
  TTAATGCCAACTACATAGATATTCGGATAAACCGAGAAGGTTACCACAGGTCAAACCACTTCATAGCCAC
  TCAAGGGCCGAAGCCTGAGATGGTCTATGACTTCTGGCGTATGGTGTGGCAGGAGCACTGTTCCAGCATC
  GTCATGATCACCAAGCTGGTCGAGGTGGGCAGGGTGAAATGCTCACGGTACTGGCCGGAGGACTCAGACA
  CCTACGGGGACATCAAGATTATGCTGGTGAAGACAGAGACCCTGGCTGAGTATGTCGTGCGCACTTTTGC
  CCTGGAGCGGAGAGGCTACTCTGCCCGGCACGAGGTCCGCCAGTTCCACTTCACAGCGTGGCCAGAGCAT
  GGCGTCCCCTACCATGCCACGGGGCTGCTGGCTTTCATCCGGCGCGTGAAGGCCTCCACCCCACCTGATG
  CCGGGCCCATTGTCATCCACTGCAGCGCGGGCACCGGCCGCACAGGTTGCTATATCGTCCTGGATGTGAT
  GCTGGACATGGCAGAGTGTGAGGGCGTCGTGGACATTTACAACTGTGTGAAGACTCTCTGCTCCCGGCGT
  GTCAACATGATCCAGACTGAGGAGCAGTACATCTTCATTCATGATGCAATCCTGGAGGCCTGCCTGTGTG
  GGGAGACCACCATCCCTGTCAGTGAGTTCAAGGCCACCTACAAGGAGATGATCCGCATTGATCCTCAGAG
  TAATTCCTCCCAGCTGCGGGAAGAGTTCCAGACGCTGAACTCGGTCACCCCGCCGCTGGACGTGGAGGAG
  TGCAGCATCGCCCTGTTGCCCCGGAACCGCGACAAGAACCGCAGCATGGACGTCCTGCCGCCCGACCGCT
  GCCTGCCCTTCCTCATCTCCACTGATGGGGACTCCAACAACTACATTAATGCAGCCCTGACTGACAGCTA
  CACACGGAGTGCGGCCTTCATCGTGACCCTGCACCCGCTGCAGAGCACCACGCCCGACTTCTGGCGGCTG
  GTCTACGATTACGGGTGCACCTCCATCGTCATGCTCAACCAGCTGAACCAGTCCAACTCCGCCTGGCCCT
  GCCTGCAGTACTGGCCAGAGCCAGGCCGGCAGCAATATGGCCTCATGGAGGTGGAGTTTATGTCGGGCAC
  AGCTGATGAAGACTTAGTGGCTCGAGTCTTCCGGGTGCAGAACATCTCTCGGGAGGGGCACCTGCTGGTG
  CGGCACTTCCAGTTCCTGCGCTGGTCTGCATACCGGGACACACCTGACTCCAAGAAGGCCTTCTTGCACC
  TGCTGGCTGAGGTGGACAAGTGGCAGGCCGAGAGTGGGGATGGGCGCACCATCGTGCACTGCCTAAACGG
  GGGAGGACGCAGCGGCACCTTCTGCGCCTGCGCCACGGTCCTGGAGATGATCCGCTGCCACAACTTGGTG
  GACGTTTTCTTTGCTGCCAAAACCCTCCGGAACTACAAACCCAACATGGTGGAGACCATGGATCAGTACC
  ACTTTTGCTACGATGTGGCCCTGGAGTACTTGGAGGGGCTGGAGTCAAGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214198 representing NM_133177
  Red=Cloning site Green=Tags(s)
  
  MARAQALVLALTFQLCAPETETPAAGCTFEEASDPAVPCEYSQAQYDDFQWEQVRIHPGTRAPADLPHGS
  YLMVNTSQHAPGQRAHVIFQSLSENDTHCVQFSYFLYSRDGHSPGTLGVYVRVNGGPLGSAVWNMTGSHG
  RQWHQAELAVSTFWPNEYQVLFEALISPDRRGYMGLDDILLLSYPCAKAPHFSRLGDVEVNAGQNASFQC
  MAAGRAAEAERFLLQRQSGALVPAAGVRHISHRRFLATFPLAAVSRAEQDLYRCVSQAPRGAGVSNFAEL
  IVKEPPTPIAPPQLLRAGPTYLIIQLNTNSIIGDGPIVRKEIEYRMARGPWAEVHAVSLQTYKLWHLDPD
  TEYEISVLLTRPGDGGTGRPGPPLISRTKCAEPMRAPKGLAFAEIQARQLTLQWEPLGYNVTRCHTYTVS
  LCYHYTLGSSHNQTIRECVKTEQGVSRYTIKNLLPYRNVHVRLVLTNPEGRKEGKEVTFQTDEDVPSGIA
  AESLTFTPLEDMIFLKWEEPQEPNGLITQYEISYQSIESSDPAVNVPGPRRTISKLRNETYHVFSNLHPG
  TTYLFSVRARTGKGFGQAALTEITTNISAPSFDYADMPSPLGESENTITVLLRPAQGRGAPISVYQVIVE
  EERARRLRREPGGQDCFPVPLTFEAALARGLVHYFGAELAASSLPEAMPFTVGDNQTYRGFWNPPLEPRK
  AYLIYFQAASHLKGETRLNCIRIARKAACKESKRPLEVSQRSEEMGLILGICAGGLAVLILLLGAIIVII
  RKGKPVNMTKATVNYRQEKTHMMSAVDRSFTDQSTLQEDERLGLSFMDTHGYSTRGDQRSGGVTEASSLL
  GGSPRRPCGRKGSPYHTGQLHPAVRVADLLQHINQMKTAEGYGFKQEYESFFEGWDATKKKDKVKGSRQE
  PMPAYDRHRVKLHPMLGDPNADYINANYIDIRINREGYHRSNHFIATQGPKPEMVYDFWRMVWQEHCSSI
  VMITKLVEVGRVKCSRYWPEDSDTYGDIKIMLVKTETLAEYVVRTFALERRGYSARHEVRQFHFTAWPEH
  GVPYHATGLLAFIRRVKASTPPDAGPIVIHCSAGTGRTGCYIVLDVMLDMAECEGVVDIYNCVKTLCSRR
  VNMIQTEEQYIFIHDAILEACLCGETTIPVSEFKATYKEMIRIDPQSNSSQLREEFQTLNSVTPPLDVEE
  CSIALLPRNRDKNRSMDVLPPDRCLPFLISTDGDSNNYINAALTDSYTRSAAFIVTLHPLQSTTPDFWRL
  VYDYGCTSIVMLNQLNQSNSAWPCLQYWPEPGRQQYGLMEVEFMSGTADEDLVARVFRVQNISREGHLLV
  RHFQFLRWSAYRDTPDSKKAFLHLLAEVDKWQAESGDGRTIVHCLNGGGRSGTFCACATVLEMIRCHNLV
  DVFFAAKTLRNYKPNMVETMDQYHFCYDVALEYLEGLESR
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133177
• ORF Size: 4320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133177.4
• RefSeq Size: 5614 bp
• RefSeq ORF: 4323 bp
• Locus ID: 10076
• UniProt ID: Q92729
• Cytogenetics: 1p35.3
• Protein Families: Transmembrane
• Gene Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracellular catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP (MAM) domain, Ig-like and fibronectin type III-like repeats. This PTP was thought to play roles in cell-cell recognition and adhesion. Studies of the similar gene in mice suggested the role of this PTP in early neural development. The expression of this gene was reported to be regulated by phorbol myristate acetate (PMA) or calcium ionophore in Jurkat T lymphoma cells. Alternatively spliced transcript variants have been reported. [provided by RefSeq, Aug 2010]