Protocadherin 21 (CDHR1) (NM_033100) Human Tagged ORF Clone

CAT#: RG213783

CDHR1 (tGFP-tagged) - Human cadherin-related family member 1 (CDHR1), transcript variant 1

Product Images

Circular map for RG213783

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Protocadherin 21
• Synonyms: CORD15; PCDH21; PRCAD; RP65
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213783 representing NM_033100
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGCTGCCGGTGGGCCGCCCTGGCCCTGGGGCTGCTGCGCCTCTGCTTGGCTCAGGCCAACTTCG
  CCCCGCACTTCTTCGACAACGGGGTCGGCAGCACCAACGGAAACATGGCTCTGTTCAGCCTCCCAGAGGA
  CACCCCTGTAGGCTCTCACGTATACACCCTGAATGGGACAGACCCTGAGGGAGACCCCATCTCCTACCAC
  ATCAGCTTTGACCCCAGCACTAGAAGCGTCTTTTCTGTTGACCCCACTTTTGGAAACATCACCCTGGTTG
  AAGAGCTGGACAGAGAGAGGGAAGATGAGATTGAAGCCATCATCAGCATTTCTGATGGCCTGAATCTGGT
  GGCCGAAAAAGTCGTGATCCTGGTGACCGATGCCAATGATGAGGCGCCCAGGTTCATCCAGGAGCCTTAT
  GTTGCCCTGGTTCCCGAGGACATACCTGCTGGGAGCATCATCTTTAAGGTCCATGCAGTGGACAGGGACA
  CAGGCTCTGGAGGGAGTGTCACCTACTTCCTGCAGAACCTGCACTCCCCATTTGCCGTGGACCGCCACAG
  CGGTGTGCTGCGCCTCCAGGCTGGGGCCACTCTGGACTACGAGAGGTCCCGGACCCACTACATCACCGTG
  GTCGCCAAGGATGGCGGTGGGAGGCTTCATGGGGCTGATGTGGTGTTCTCAGCCACCACCACGGTCACGG
  TCAATGTGGAGGATGTTCAGGACATGGCCCCTGTCTTCGTGGGCACACCCTACTATGGCTATGTGTACGA
  GGACACCCTTCCGGGCTCGGAGGTACTGAAGGTGGTCGCCATGGATGGAGACCGGGGCAAACCCAATCGA
  ATTCTCTACAGCCTTGTAAATGGGAACGATGGAGCCTTTGAAATTAATGAGACATCTGGAGCCATCTCCA
  TCACTCAGAGCCCGGCCCAGCTCCAGAGAGAGGTGTATGAGCTGCATGTACAGGTGACTGAAATGAGCCC
  TGCGGGGAGCCCAGCTGCCCAGGCCACCGTCCCAGTCACCATCAGGATTGTGGACCTCAACAACCACCCG
  CCAACATTCTATGGAGAGAGCGGACCCCAAAACAGGTTTGAGCTGTCCATGAATGAGCACCCACCCCAGG
  GAGAGATCCTGCGGGGCCTCAAGATCACCGTCAATGACTCCGACCAGGGAGCCAATGCCAAATTCAACTT
  GCAGCTGGTGGGACCCAGGGGCATCTTCCGAGTGGTTCCACAGACAGTCCTGAATGAAGCCCAAGTCACA
  ATCATTGTGGAGAACTCAGCTGCCATTGACTTTGAAAAGTCCAAAGTATTAACCTTCAAGCTCCTGGCTG
  TTGAAGTGAACACCCCAGAGAAGTTCAGTTCCACAGCGGATGTTGTGATCCAGCTCCTGGACACCAATGA
  CAATGTCCCCAAGTTCGACTCCCTCTACTACGTTGCCAGGATTCCTGAGAACGCCCCAGGGGGCTCCAGC
  GTGGTGGCTGTCACAGCTGTGGATCCAGATACAGGACCCTGGGGCGAAGTGAAATATTCCACCTATGGGA
  CTGGGGCAGACCTCTTCCTGATCCACCCATCCACTGGGCTTATCTACACCCAGCCCTGGGCTAGCCTGGA
  CGCTGAGGCCACTGCCAGGTACAACTTCTATGTGAAGGCAGAGGACATGGAAGGCAAGTACAGCGTAGCT
  GAGGTGTTTATCACACTGCTGGATGTCAATGACCACCCCCCTCAGTTTGGAAAGAGCGTTCAGAAGAAGA
  CGATGGTGCTAGGGACCCCAGTGAAAATTGAGGCCATAGACGAGGATGCAGAGGAACCCAACAACCTGGT
  GGACTATTCCATCACCCATGCAGAGCCCGCCAACGTGTTCGACATCAATTCCCACACGGGGGAGATCTGG
  CTCAAGAATTCCATCCGCTCCCTGGATGCCCTGCACAACATCACACCTGGAAGGGACTGCCTATGGTCCC
  TAGAGGTGCAGGCCAAGGACCGGGGCTCCCCATCCTTCAGCACCACAGCCTTACTCAAGATTGACATCAC
  AGATGCTGAGACCCTCTCCCGGAGCCCCATGGCTGCCTTCCTGATACAGACCAAGGACAACCCCATGAAG
  GCCGTGGGTGTGCTGGCCGGCACCATGGCCACCGTCGTGGCCATCACTGTCCTCATCTCCACCGCCACCT
  TCTGGCGCAACAAGAAGTCTAACAAGGTCCTGCCAATGCGGCGGGTGCTCCGCAAGCGGCCCAGCCCTGC
  GCCCCGCACCATCCGCATTGAGTGGCTCAAGTCCAAGAGCACCAAAGCCGCTACCAAGTTCATGCTCAAA
  GAGAAACCTCCCAATGAGAACTGTAACAACAACAGCCCAGAAAGCTCTCTGCTCCCGAGAGCTCCGGCTC
  TCCCTCCACCACCCAGCGTGGCGCCCAGCACTGGCGCAGCCCAGTGGACCGTGCCTACTGTCTCTGGCTC
  TCTCACTCCGCAGCCGACCCAACCCCCGCCAAAACCCAAAACTATGGGAAGCCCCGTCCAGTCAACTCTG
  ATCTCTGAGCTCAAGCAAAAGTTTGAGAAGAAGAGTGTGCACAACAAGGCTTACTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213783 representing NM_033100
  Red=Cloning site Green=Tags(s)
  
  MRRCRWAALALGLLRLCLAQANFAPHFFDNGVGSTNGNMALFSLPEDTPVGSHVYTLNGTDPEGDPISYH
  ISFDPSTRSVFSVDPTFGNITLVEELDREREDEIEAIISISDGLNLVAEKVVILVTDANDEAPRFIQEPY
  VALVPEDIPAGSIIFKVHAVDRDTGSGGSVTYFLQNLHSPFAVDRHSGVLRLQAGATLDYERSRTHYITV
  VAKDGGGRLHGADVVFSATTTVTVNVEDVQDMAPVFVGTPYYGYVYEDTLPGSEVLKVVAMDGDRGKPNR
  ILYSLVNGNDGAFEINETSGAISITQSPAQLQREVYELHVQVTEMSPAGSPAAQATVPVTIRIVDLNNHP
  PTFYGESGPQNRFELSMNEHPPQGEILRGLKITVNDSDQGANAKFNLQLVGPRGIFRVVPQTVLNEAQVT
  IIVENSAAIDFEKSKVLTFKLLAVEVNTPEKFSSTADVVIQLLDTNDNVPKFDSLYYVARIPENAPGGSS
  VVAVTAVDPDTGPWGEVKYSTYGTGADLFLIHPSTGLIYTQPWASLDAEATARYNFYVKAEDMEGKYSVA
  EVFITLLDVNDHPPQFGKSVQKKTMVLGTPVKIEAIDEDAEEPNNLVDYSITHAEPANVFDINSHTGEIW
  LKNSIRSLDALHNITPGRDCLWSLEVQAKDRGSPSFSTTALLKIDITDAETLSRSPMAAFLIQTKDNPMK
  AVGVLAGTMATVVAITVLISTATFWRNKKSNKVLPMRRVLRKRPSPAPRTIRIEWLKSKSTKAATKFMLK
  EKPPNENCNNNSPESSLLPRAPALPPPPSVAPSTGAAQWTVPTVSGSLTPQPTQPPPKPKTMGSPVQSTL
  ISELKQKFEKKSVHNKAYF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033100
• ORF Size: 2577 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033100.4
• RefSeq Size: 5337 bp
• RefSeq ORF: 2580 bp
• Locus ID: 92211
• UniProt ID: Q96JP9
• Cytogenetics: 10q23.1
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene belongs to the cadherin superfamily of calcium-dependent cell adhesion molecules. The encoded protein is a photoreceptor-specific cadherin that plays a role in outer segment disc morphogenesis. Mutations in this gene are associated with inherited retinal dystrophies. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2013]