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SBF2 (NM_030962) Human Tagged ORF Clone
CAT#: RG213601
- TrueORF®
SBF2 (tGFP-tagged) - Human SET binding factor 2 (SBF2)
ORF Plasmid: DDK
"NM_030962" in other vectors (2)
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Symbol | SBF2 |
| Synonyms | CMT4B2; DENND7B; MTMR13 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213601 representing NM_030962
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGGCTGGCTGACTACTTCATCGTGGTAGGCTATGACCACGAGAAGCCAGGATCAGGAGAAGGTC TGGGGAAAATAATCCAGAGATTTCCACAGAAGGACTGGGATGATACACCTTTTCCACAGGGAATTGAGTT GTTTTGTCAGCCTGGCGGGTGGCAGCTGTCCAGAGAGAGGAAGCAGCCAACGTTCTTTGTGGTTGTCCTG ACAGACATTGACTCAGATCGACATTACTGCTCATGCCTAACCTTCTATGAGGCAGAGATCAATCTTCAGG GAACAAAGAAGGAAGAGATTGAAGGTGAAGCAAAAGTGTCTGGTTTAATTCAGCCTGCAGAAGTGTTTGC TCCCAAAAGCCTGGTGTTGGTATCCAGATTATATTATCCAGAAATTTTTAGGGCTTGCCTGGGTTTGATC TATACCGTGTATGTGGACAGCCTGAATGTCTCCTTGGAAAGTCTAATTGCAAACCTTTGTGCCTGCCTTG TCCCAGCGGCTGGAGGGTCTCAGAAGCTGTTTTCTTTGGGTGCAGGAGATAGACAGTTGATCCAGACTCC TTTACATGATAGTCTTCCTATCACGGGCACTAGTGTGGCTCTCCTGTTCCAGCAATTGGGAATTCAAAAT GTCCTCAGCCTCTTTTGTGCAGTCCTCACAGAAAATAAGGTTCTCTTCCATTCTGCAAGTTTCCAGAGAC TTAGTGATGCTTGTAGAGCCCTGGAATCTTTAATGTTTCCTCTTAAATATAGTTATCCTTATATCCCTAT TCTCCCGGCTCAGCTACTGGAAGTTCTAAGTTCCCCAACGCCTTTCATTATTGGAGTACATTCTGTCTTT AAAACTGATGTCCATGAACTTTTAGATGTAATCATAGCAGATTTGGATGGAGGCACTATTAAAATTCCCG AATGTATTCACCTCTCTTCCCTCCCAGAACCACTTCTACATCAGACTCAATCAGCTCTTTCTTTGATTTT ACACCCAGATTTGGAAGTAGCAGATCATGCTTTTCCTCCTCCACGAACAGCTTTATCCCACTCAAAAATG CTGGATAAAGAGGTGCGAGCCGTTTTCCTTAGATTATTTGCACAACTCTTCCAAGGATATAGATCCTGCC TGCAACTTATAAGAATTCATGCAGAGCCAGTAATACATTTCCACAAGACAGCATTCTTGGGGCAGCGTGG TTTGGTCGAGAATGATTTCCTCACTAAAGTACTCAGTGGAATGGCATTTGCAGGTTTTGTTTCAGAAAGA GGTCCTCCTTATAGATCTTGTGATCTCTTTGATGAGTTGGTAGCCTTTGAAGTAGAGAGAATTAAAGTTG AAGAAAATAACCCAGTGAAGATGATAAAGCATGTCAGGGAACTTGCTGAGCAACTATTCAAAAATGAGAA TCCAAATCCTCATATGGCATTCCAGAAAGTTCCACGGCCAACAGAAGGATCCCATTTGCGAGTTCATATT CTTCCTTTCCCAGAGATTAATGAAGCCCGGGTTCAGGAATTAATACAGGAAAATGTTGCTAAGAACCAGA ATGCACCTCCTGCCACACGAATAGAAAAGAAATGTGTTGTGCCAGCAGGTCCACCTGTTGTTTCGATAAT GGACAAGGTGACGACAGTTTTCAACAGTGCACAAAGACTAGAAGTTGTCAGAAACTGTATCTCATTCATA TTTGAAAATAAAATTTTGGAAACTGAAAAGACCCTTCCTGCTGCACTCAGAGCCCTTAAAGGAAAGGCAG CAAGACAGTGTCTCACTGATGAATTGGGTTTGCATGTCCAGCAAAACCGGGCAATATTAGACCATCAACA GTTTGACTACATAATAAGGATGATGAATTGTACTCTACAGGATTGTTCAAGTTTAGAAGAATACAACATT GCCGCAGCATTACTCCCTTTGACCAGTGCTTTCTATAGGAAACTTGCCCCTGGAGTCAGCCAGTTTGCTT ACACGTGTGTACAAGACCACCCCATTTGGACAAATCAGCAATTTTGGGAGACAACCTTTTACAATGCAGT GCAGGAACAGGTTCGCTCCCTTTATCTCTCAGCCAAGGAAGACAATCATGCCCCGCATCTGAAGCAAAAG GATAAGCTTCCTGATGACCATTATCAGGAGAAGACAGCAATGGACCTGGCAGCTGAGCAACTACGCCTTT GGCCTACCCTGAGCAAGTCAACTCAGCAAGAGCTAGTGCAACATGAGGAAAGCACTGTCTTTAGTCAGGC CATTCACTTTGCAAACCTCATGGTGAACCTGCTAGTTCCACTCGACACAAGTAAAAACAAGCTCCTAAGA ACATCAGCGCCAGGTGACTGGGAGAGCGGAAGCAACAGCATTGTCACAAACAGTATTGCAGGAAGTGTAG CTGAGAGCTATGATACAGAGAGTGGGTTTGAAGATTCAGAGAATACTGACATTGCCAATTCTGTTGTGCG GTTCATTACCCGATTTATTGACAAAGTTTGTACAGAGAGTGGAGTTACTCAGGATCACATCAAGAGCCTT CATTGCATGATACCAGGAATTGTAGCTATGCACATTGAGACCCTAGAAGCAGTACATCGAGAAAGCAGAA GACTTCCGCCTATTCAGAAGCCCAAGATTCTTAGACCTGCTCTGCTGCCAGGAGAAGAAATTGTCTGTGA GGGTCTTCGAGTCTTGCTGGATCCTGATGGAAGAGAAGAAGCTACTGGAGGTCTTCTTGGAGGCCCTCAG CTCCTGCCAGCAGAAGGAGCCTTGTTCCTCACCACATACAGAATTCTCTTCAGAGGAACACCCCATGATC AGTTAGTGGGTGAGCAGACAGTTGTGCGGAGCTTTCCCATTGCCTCCATCACCAAGGAGAAGAAGATTAC AATGCAGAACCAGCTACAGCAGAACATGCAAGAAGGACTGCAGATCACATCAGCATCTTTTCAGTTGATT AAGGTAGCATTTGATGAAGAAGTCAGTCCAGAAGTAGTAGAGATCTTTAAGAAACAGCTGATGAAGTTCC GTTATCCTCAGTCCATTTTCAGTACCTTTGCTTTTGCTGCTGGACAAACTACCCCACAAATAATTTTACC AAAACAGAAGGAAAAGAACACTTCTTTTCGTACCTTCTCAAAAACAATTGTGAAAGGTGCCAAAAGGGCA GGGAAAATGACAATTGGGCGGCAATATTTACTGAAGAAGAAGACAGGGACAATTGTGGAAGAAAGAGTAA ATCGTCCTGGATGGAATGAAGATGATGATGTATCTGTTTCAGATGAGAGTGAGCTCCCCACAAGTACCAC CCTGAAGGCCTCCGAGAAGTCTACAATGGAACAGTTGGTGGAAAAAGCTTGTTTCAGAGACTATCAGCGT TTAGGTTTAGGAACCATAAGTGGCAGCTCTTCCCGTTCAAGACCCGAGTATTTTAGAATTACTGCCTCCA ACAGGATGTATTCACTCTGCCGGAGCTATCCTGGCCTTTTAGTCGTACCTCAAGCTGTACAGGACAGTAG TTTACCAAGAGTAGCTCGCTGCTATCGACACAATCGCCTGCCTGTTGTATGTTGGAAGAACTCAAGAAGT GGTACTCTGCTCCTCCGATCTGGAGGATTCCATGGGAAGGGAGTCGTTGGTCTTTTCAAATCTCAGAACT CCCCTCAGGCCGCTCCTACCTCCTCTTTAGAATCTTCCAGTAGCATAGAACAAGAGAAATACTTGCAAGC CTTACTGAATGCTGTTTCTGTCCATCAGAAACTCAGAGGCAACAGCACTCTTACTGTCAGGCCAGCCTTT GCTCTATCTCCAGGTGTGTGGGCAAGTCTTCGCTCTAGCACTCGCTTGATCAGCTCTCCAACATCCTTCA TTGATGTTGGCGCCCGGCTGGCAGGCAAGGATCACTCGGCCTCCTTCAGTAACAGCAGCTACCTACAAAA CCAGCTCTTGAAACGGCAAGCAGCCCTTTACATATTTGGTGAAAAGTCGCAACTAAGGAACTTCAAGGTA GAATTTGCTTTAAATTGTGAGTTTGTTCCTGTTGAATTTCATGAAATCCGGCAAGTGAAAGCCAGTTTTA AGAAGCTGATGAGGGCTTGTATCCCAAGCACCATCCCTACTGACTCAGAAGTGACCTTCCTGAAAGCGCT GGGAGATTCTGAGTGGTTCCCACAGCTTCACAGGATAATGCAGCTGGCTGTGGTTGTATCAGAAGTACTT GAGAATGGTTCCTCAGTTTTGGTCTGTTTGGAGGAAGGCTGGGACATCACTGCACAAGTGACATCCCTGG TTCAGTTACTCAGTGATCCCTTTTATAGGACACTTGAAGGCTTCCAGATGTTGGTTGAAAAAGAGTGGCT CTCTTTTGGTCACAAATTCAGTCAGAGGAGCAGCTTGACCCTCAACTGTCAGGGGAGTGGTTTTGCTCCA GTCTTCTTACAGTTCTTAGACTGTGTACACCAGGTTCACAACCAGTATCCAACTGAGTTTGAATTCAATC TCTATTACTTAAAGTTCTTGGCTTTCCACTATGTGTCTAATCGCTTTAAAACATTTCTCCTGGATTCAGA CTATGAAAGATTAGAGCACGGAACTTTATTTGATGATAAAGGAGAAAAGCATGCCAAAAAAGGAGTCTGT ATTTGGGAATGTATTGACAGAATGCACAAGAGGAGTCCCATTTTCTTTAATTATTTATATTCACCATTGG AAATAGAGGCTCTAAAGCCCAATGTAAACGTCTCTAGCCTCAAGAAGTGGGATTACTACATAGAAGAGAC CCTGTCCACAGGCCCTTCCTATGACTGGATGATGCTAACCCCCAAGCACTTCCCCTCCGAAGACTCTGAC CTGGCTGGAGAAGCTGGGCCACGGAGCCAGAGGAGAACAGTGTGGCCATGCTATGATGATGTCAGCTGTA CTCAGCCTGATGCTCTCACCAGCCTTTTCAGTGAAATTGAAAAATTGGAGCACAAATTGAACCAAGCCCC TGAGAAGTGGCAGCAGCTGTGGGAAAGGGTAACCGTGGACCTTAAAGAAGAACCAAGAACAGATCGCTCC CAAAGACACCTGTCGAGATCCCCAGGAATTGTGTCTACCAACCTACCTTCCTATCAGAAGAGGTCTCTGC TACATCTCCCAGACAGCAGCATGGGGGAGGAACAGAATTCCAGCATCTCCCCATCCAATGGAGTGGAGCG AAGAGCAGCCACGCTCTATAGCCAGTATACATCCAAGAATGATGAAAACAGGTCCTTTGAGGGAACACTT TATAAAAGAGGGGCTTTGCTGAAAGGTTGGAAGCCCCGTTGGTTTGTTTTGGATGTAACAAAACATCAGC TGCGCTACTATGACTCAGGTGAGGACACAAGCTGTAAAGGCCACATTGATCTGGCTGAAGTAGAAATGGT CATCCCTGCTGGCCCCAGCATGGGAGCCCCAAAGCACACAAGTGACAAGGCTTTCTTTGATCTCAAGACC AGCAAACGTGTGTATAACTTCTGCGCCCAGGATGGACAGAGTGCCCAGCAATGGATGGACAAGATCCAGA GTTGTATCTCTGATGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213601 representing NM_030962
Red=Cloning site Green=Tags(s) MARLADYFIVVGYDHEKPGSGEGLGKIIQRFPQKDWDDTPFPQGIELFCQPGGWQLSRERKQPTFFVVVL TDIDSDRHYCSCLTFYEAEINLQGTKKEEIEGEAKVSGLIQPAEVFAPKSLVLVSRLYYPEIFRACLGLI YTVYVDSLNVSLESLIANLCACLVPAAGGSQKLFSLGAGDRQLIQTPLHDSLPITGTSVALLFQQLGIQN VLSLFCAVLTENKVLFHSASFQRLSDACRALESLMFPLKYSYPYIPILPAQLLEVLSSPTPFIIGVHSVF KTDVHELLDVIIADLDGGTIKIPECIHLSSLPEPLLHQTQSALSLILHPDLEVADHAFPPPRTALSHSKM LDKEVRAVFLRLFAQLFQGYRSCLQLIRIHAEPVIHFHKTAFLGQRGLVENDFLTKVLSGMAFAGFVSER GPPYRSCDLFDELVAFEVERIKVEENNPVKMIKHVRELAEQLFKNENPNPHMAFQKVPRPTEGSHLRVHI LPFPEINEARVQELIQENVAKNQNAPPATRIEKKCVVPAGPPVVSIMDKVTTVFNSAQRLEVVRNCISFI FENKILETEKTLPAALRALKGKAARQCLTDELGLHVQQNRAILDHQQFDYIIRMMNCTLQDCSSLEEYNI AAALLPLTSAFYRKLAPGVSQFAYTCVQDHPIWTNQQFWETTFYNAVQEQVRSLYLSAKEDNHAPHLKQK DKLPDDHYQEKTAMDLAAEQLRLWPTLSKSTQQELVQHEESTVFSQAIHFANLMVNLLVPLDTSKNKLLR TSAPGDWESGSNSIVTNSIAGSVAESYDTESGFEDSENTDIANSVVRFITRFIDKVCTESGVTQDHIKSL HCMIPGIVAMHIETLEAVHRESRRLPPIQKPKILRPALLPGEEIVCEGLRVLLDPDGREEATGGLLGGPQ LLPAEGALFLTTYRILFRGTPHDQLVGEQTVVRSFPIASITKEKKITMQNQLQQNMQEGLQITSASFQLI KVAFDEEVSPEVVEIFKKQLMKFRYPQSIFSTFAFAAGQTTPQIILPKQKEKNTSFRTFSKTIVKGAKRA GKMTIGRQYLLKKKTGTIVEERVNRPGWNEDDDVSVSDESELPTSTTLKASEKSTMEQLVEKACFRDYQR LGLGTISGSSSRSRPEYFRITASNRMYSLCRSYPGLLVVPQAVQDSSLPRVARCYRHNRLPVVCWKNSRS GTLLLRSGGFHGKGVVGLFKSQNSPQAAPTSSLESSSSIEQEKYLQALLNAVSVHQKLRGNSTLTVRPAF ALSPGVWASLRSSTRLISSPTSFIDVGARLAGKDHSASFSNSSYLQNQLLKRQAALYIFGEKSQLRNFKV EFALNCEFVPVEFHEIRQVKASFKKLMRACIPSTIPTDSEVTFLKALGDSEWFPQLHRIMQLAVVVSEVL ENGSSVLVCLEEGWDITAQVTSLVQLLSDPFYRTLEGFQMLVEKEWLSFGHKFSQRSSLTLNCQGSGFAP VFLQFLDCVHQVHNQYPTEFEFNLYYLKFLAFHYVSNRFKTFLLDSDYERLEHGTLFDDKGEKHAKKGVC IWECIDRMHKRSPIFFNYLYSPLEIEALKPNVNVSSLKKWDYYIEETLSTGPSYDWMMLTPKHFPSEDSD LAGEAGPRSQRRTVWPCYDDVSCTQPDALTSLFSEIEKLEHKLNQAPEKWQQLWERVTVDLKEEPRTDRS QRHLSRSPGIVSTNLPSYQKRSLLHLPDSSMGEEQNSSISPSNGVERRAATLYSQYTSKNDENRSFEGTL YKRGALLKGWKPRWFVLDVTKHQLRYYDSGEDTSCKGHIDLAEVEMVIPAGPSMGAPKHTSDKAFFDLKT SKRVYNFCAQDGQSAQQWMDKIQSCISDA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030962 |
| ORF Size | 5547 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030962.3, NP_112224.1 |
| RefSeq Size | 7447 bp |
| RefSeq ORF | 5550 bp |
| Locus ID | 81846 |
| UniProt ID | Q86WG5 |
| Cytogenetics | 11p15.4 |
| Gene Summary | This gene encodes a pseudophosphatase and member of the myotubularin-related protein family. This gene maps within the CMT4B2 candidate region of chromosome 11p15 and mutations in this gene have been associated with Charcot-Marie-Tooth Disease, type 4B2. [provided by RefSeq, Jul 2008] |
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