PSIP1 (NM_021144) Human Tagged ORF Clone
CAT#: RG212915
- TrueORF®
PSIP1 (tGFP-tagged) - Human PC4 and SFRS1 interacting protein 1 (PSIP1), transcript variant 1
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_021144" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | PSIP1 |
Synonyms | DFS70; LEDGF; p52; p75; PAIP; PSIP2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG212915 representing NM_021144
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTCGCGATTTCAAACCTGGAGACCTCATCTTCGCCAAGATGAAAGGTTATCCCCATTGGCCAGCTC GAGTAGACGAAGTTCCTGATGGAGCTGTAAAGCCACCCACAAACAAACTACCCATTTTCTTTTTTGGAAC TCATGAGACTGCTTTTTTAGGACCAAAGGATATATTTCCTTACTCAGAAAATAAGGAAAAGTATGGCAAA CCAAATAAAAGAAAAGGTTTTAATGAAGGTTTATGGGAGATAGATAACAATCCAAAAGTGAAATTTTCAA GTCAACAGGCAGCAACTAAACAATCAAATGCATCATCTGATGTTGAAGTTGAAGAAAAGGAAACTAGTGT TTCAAAGGAAGATACCGACCATGAAGAAAAAGCCAGCAATGAGGATGTGACTAAAGCAGTTGACATAACT ACTCCAAAAGCTGCCAGAAGGGGGAGAAAGAGAAAGGCAGAAAAACAAGTAGAAACTGAGGAGGCAGGAG TAGTGACAACAGCAACAGCATCTGTTAATCTAAAAGTGAGTCCTAAAAGAGGACGACCTGCAGCTACAGA AGTCAAGATTCCAAAACCAAGAGGCAGACCCAAAATGGTAAAACAGCCCTGTCCTTCAGAGAGTGACATC ATTACTGAAGAGGACAAAAGTAAGAAAAAGGGGCAAGAGGGAAAACAACCTAAAAAGCAGCCTAAGAAGG ATGAAGAGGGCCAGAAGGAAGAAGATAAGCCAAGAAAAGAGCCGGATAAAAAAGAGGGGAAGAAAGAAGT TGAATCAAAAAGGAAAAATTTAGCTAAAACAGGGGTTACTTCAACCTCCGATTCTGAAGAAGAAGGAGAT GATCAAGAAGGTGAAAAGAAGAGAAAAGGTGGGAGGAACTTTCAGACTGCTCACAGAAGGAATATGCTGA AAGGCCAACATGAGAAAGAAGCAGCAGATCGAAAACGCAAGCAAGAGGAACAAATGGAAACTGAGCACCA AACAACATGTAATCTACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212915 representing NM_021144
Red=Cloning site Green=Tags(s) MTRDFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGK PNKRKGFNEGLWEIDNNPKVKFSSQQAATKQSNASSDVEVEEKETSVSKEDTDHEEKASNEDVTKAVDIT TPKAARRGRKRKAEKQVETEEAGVVTTATASVNLKVSPKRGRPAATEVKIPKPRGRPKMVKQPCPSESDI ITEEDKSKKKGQEGKQPKKQPKKDEEGQKEEDKPRKEPDKKEGKKEVESKRKNLAKTGVTSTSDSEEEGD DQEGEKKRKGGRNFQTAHRRNMLKGQHEKEAADRKRKQEEQMETEHQTTCNLQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021144 |
ORF Size | 999 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021144.2, NP_066967.2 |
RefSeq Size | 1677 bp |
RefSeq ORF | 1002 bp |
Locus ID | 11168 |
UniProt ID | O75475 |
Cytogenetics | 9p22.3 |
Domains | PWWP |
Protein Families | Stem cell - Pluripotency, Transcription Factors |
Gene Summary | Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212915 | PSIP1 (Myc-DDK-tagged)-Human PC4 and SFRS1 interacting protein 1 (PSIP1), transcript variant 1 |
USD 450.00 |
|
RC212915L1 | Lenti-ORF clone of PSIP1 (Myc-DDK-tagged)-Human PC4 and SFRS1 interacting protein 1 (PSIP1), transcript variant 1 |
USD 750.00 |
|
RC212915L2 | Lenti-ORF clone of PSIP1 (mGFP-tagged)-Human PC4 and SFRS1 interacting protein 1 (PSIP1), transcript variant 1 |
USD 750.00 |
|
RC212915L3 | Lenti-ORF clone of PSIP1 (Myc-DDK-tagged)-Human PC4 and SFRS1 interacting protein 1 (PSIP1), transcript variant 1 |
USD 750.00 |
|
RC212915L4 | Lenti-ORF clone of PSIP1 (mGFP-tagged)-Human PC4 and SFRS1 interacting protein 1 (PSIP1), transcript variant 1 |
USD 750.00 |
|
SC312998 | PSIP1 (untagged)-Human PC4 and SFRS1 interacting protein 1 (PSIP1), transcript variant 1 |
USD 686.00 |
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