SP8 (NM_182700) Human Tagged ORF Clone

CAT#: RG212880

SP8 (tGFP-tagged) - Human Sp8 transcription factor (SP8), transcript variant 1

Product Images

Circular map for RG212880

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SP8
• Synonyms: BTD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212880 representing NM_182700
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACTTCACTTCTAGGGGAAGAACCGAGGTTGGGATCGACTCCTCTGGCCATGCTTGCCGCTACCT
  GTAATAAGATAGGCAGCCCCAGCCCGTCTCCCTCCTCCCTCTCGGACAGCTCTTCTTCCTTCGGCAAAGG
  CTTCCACCCCTGGAAACGCTCCTCGTCCTCTTCTTCCGCCAGCTGCAACGTAGTGGGTTCCAGTCTCTCA
  AGCTTCGGCGTGTCCGGGGCCTCCAGGAACGGCGGCTCGTCCTCGGCGGCTGCGGCGGCCGCGGCAGCAG
  CCGCGGCTGCCGCGGCCCTGGTGTCCGACTCGTTCAGCTGCGGCGGCTCGCCTGGCTCCAGCGCCTTCTC
  CCTCACCTCCAGCAGCGCCGCAGCCGCCGCCGCCGCCGCCGCAGCCGCCGCCTCCAGCTCGCCCTTCGCC
  AACGACTACTCTGTTTTCCAGGCCCCCGGAGTTTCCGGGGGCAGCGGCGGCGGCGGCGGGGGCGGCGGCG
  GCGGCTCCTCCGCGCACTCGCAGGACGGCTCCCACCAGCCGGTGTTCATCTCCAAGGTGCACACCTCTGT
  GGACGGGCTGCAGGGCATCTACCCGCGGGTGGGCATGGCGCACCCGTACGAGTCGTGGTTTAAGCCCTCG
  CACCCGGGCCTGGGTGCTGCGGGCGAGGTGGGCTCGGCCGGCGCCTCCAGCTGGTGGGACGTGGGGGCCG
  GCTGGATCGACGTGCAGAACCCGAACAGCGCGGCTGCGCTGCCCGGCTCGCTGCACCCTGCCGCCGGGGG
  GCTCCAAACCTCGCTGCACTCGCCGCTCGGAGGCTACAACTCGGATTACTCGGGCCTGAGTCACTCGGCC
  TTCAGCAGCGGCGCCTCCTCGCACCTGCTCAGCCCCGCCGGGCAGCACCTCATGGACGGCTTCAAGCCAG
  TGCTACCCGGCTCCTACCCGGACTCGGCCCCGTCGCCGCTGGCCGGCGCGGGGGGCTCCATGTTGAGCGC
  TGGGCCTTCGGCGCCGCTGGGGGGCTCCCCGCGCTCCTCAGCTCGCCGCTACTCCGGCCGCGCCACCTGC
  GACTGCCCCAACTGCCAGGAGGCAGAGCGGCTGGGCCCTGCCGGGGCGAGCTTGCGGCGCAAGGGCCTGC
  ACAGCTGCCACATCCCGGGCTGCGGCAAGGTGTACGGCAAGACTTCGCACCTCAAGGCGCACCTGCGCTG
  GCACACGGGCGAGCGGCCCTTCGTGTGCAACTGGCTTTTCTGCGGCAAGCGCTTCACGCGCTCCGACGAG
  CTGCAGCGGCACCTGCGGACCCACACCGGCGAGAAGCGCTTCGCCTGTCCAGTTTGCAACAAGCGCTTCA
  TGCGCAGCGACCACCTCAGCAAGCACGTGAAGACGCACAGTGGCGGCGGCGGCGGCGGCGGCTCGGCGGG
  CTCGGGCAGCGGCGGCAAGAAGGGCAGCGACACCGACAGCGAGCACAGCGCCGCGGGCAGCCCGCCCTGC
  CACTCCCCAGAGCTGCTGCAGCCCCCCGAGCCCGGGCACCGCAACGGCCTAGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212880 representing NM_182700
  Red=Cloning site Green=Tags(s)
  
  MATSLLGEEPRLGSTPLAMLAATCNKIGSPSPSPSSLSDSSSSFGKGFHPWKRSSSSSSASCNVVGSSLS
  SFGVSGASRNGGSSSAAAAAAAAAAAAAALVSDSFSCGGSPGSSAFSLTSSSAAAAAAAAAAAASSSPFA
  NDYSVFQAPGVSGGSGGGGGGGGGGSSAHSQDGSHQPVFISKVHTSVDGLQGIYPRVGMAHPYESWFKPS
  HPGLGAAGEVGSAGASSWWDVGAGWIDVQNPNSAAALPGSLHPAAGGLQTSLHSPLGGYNSDYSGLSHSA
  FSSGASSHLLSPAGQHLMDGFKPVLPGSYPDSAPSPLAGAGGSMLSAGPSAPLGGSPRSSARRYSGRATC
  DCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDE
  LQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTHSGGGGGGGSAGSGSGGKKGSDTDSEHSAAGSPPC
  HSPELLQPPEPGHRNGLE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182700
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182700.6
• RefSeq Size: 3670 bp
• RefSeq ORF: 1527 bp
• Locus ID: 221833
• UniProt ID: Q8IXZ3
• Cytogenetics: 7p21.1
• Gene Summary: The protein encoded by this gene is an SP family transcription factor that in mouse has been shown to be essential for proper limb development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]