CPNE1 (NM_152928) Human Tagged ORF Clone
CAT#: RG212736
- TrueORF®
CPNE1 (tGFP-tagged) - Human copine I (CPNE1), transcript variant 5
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_152928" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | CPNE1 |
Synonyms | COPN1; CPN1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG212736 representing NM_152928
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCACTGCGTGACCTTGGTTCAGCTGTCCATTTCCTGTGACCATCTCATTGACAAGGACATCGGCT CCAAGTCTGACCCACTCTGCGTCCTTTTACAGGATGTGGGAGGGGGCAGCTGGGCTGAGCTTGGCCGGAC TGAACGGGTGCGGAACTGCTCAAGCCCTGAGTTCTCCAAGACTCTACAGCTTGAGTACCGCTTTGAGACA GTCCAGAAGCTACGCTTTGGAATCTATGACATAGACAACAAGACGCCAGAGCTGAGGGATGATGACTTCC TAGGGGGTGCTGAGTGTTCCCTAGGACAGATTGTGTCCAGCCAGGTACTGACTCTCCCCTTGATGCTGAA GCCTGGAAAACCTGCTGGGCGGGGGACCATCACGGTCTCAGCTCAGGAATTAAAGGACAATCGTGTAGTA ACCATGGAGGTAGAGGCCAGAAACCTAGATAAGAAGGACTTCCTGGGAAAATCAGATCCATTTCTGGAGT TCTTCCGCCAGGGTGATGGGAAATGGCACCTGGTGTACAGATCTGAGGTCATCAAGAACAACCTGAACCC TACATGGAAGCGTTTCTCAGTCCCCGTTCAGCATTTCTGTGGTGGGAACCCCAGCACACCCATCCAGGTG CAATGCTCCGATTATGACAGTGACGGGTCACATGATCTCATCGGTACCTTCCACACCAGCTTGGCCCAGC TGCAGGCAGTCCCGGCTGAGTTTGAATGCATCCACCCTGAGAAGCAGCAGAAAAAGAAAAGCTACAAGAA CTCTGGAACTATCCGTGTCAAGATTTGTCGGGTAGAAACAGAGTACTCCTTTCTGGACTATGTGATGGGA GGCTGTCAGATCAACTTCACTGTGGGCGTGGACTTCACTGGCTCCAATGGAGACCCCTCCTCACCTGACT CCCTACACTACCTGAGTCCAACAGGGGTCAATGAGTACCTGATGGCACTGTGGAGTGTGGGCAGCGTGGT TCAGGACTATGACTCAGACAAGCTGTTCCCTGCATTTGGATTTGGGGCCCAGGTTCCCCCTGACTGGCAG GTCTCGCATGAATTTGCCTTGAATTTCAACCCCAGTAACCCCTACTGTGCAGGCATCCAGGGCATTGTGG ATGCCTACCGCCAAGCCCTGCCCCAAGTTCGCCTCTATGGCCCTACCAACTTTGCACCCATCATCAACCA TGTGGCCAGGTTTGCAGCCCAGGCTGCACATCAGGGGACTGCCTCGCAATACTTCATGCTGTTGCTGCTG ACTGATGGTGCTGTGACGGATGTGGAAGCCACACGTGAGGCTGTGGTGCGTGCCTCGAACCTGCCCATGT CAGTGATCATTGTGGGTGTGGGTGGTGCTGACTTTGAGGCCATGGAGCAGCTGGACGCTGATGGTGGACC CCTGCATACACGTTCTGGGCAGGCTGCTGCCCGCGACATTGTGCAGTTTGTACCCTACCGCCGGTTCCAG AATGCCCCTCGGGAGGCATTGGCACAGACCGTGCTCGCAGAAGTGCCCACACAACTGGTCTCATACTTCA GGGCCCAGGGTTGGGCCCCGCTCAAGCCACTTCCACCCTCAGCCAAGGATCCTGCACAGGCCCCCCAGGC C ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212736 representing NM_152928
Red=Cloning site Green=Tags(s) MAHCVTLVQLSISCDHLIDKDIGSKSDPLCVLLQDVGGGSWAELGRTERVRNCSSPEFSKTLQLEYRFET VQKLRFGIYDIDNKTPELRDDDFLGGAECSLGQIVSSQVLTLPLMLKPGKPAGRGTITVSAQELKDNRVV TMEVEARNLDKKDFLGKSDPFLEFFRQGDGKWHLVYRSEVIKNNLNPTWKRFSVPVQHFCGGNPSTPIQV QCSDYDSDGSHDLIGTFHTSLAQLQAVPAEFECIHPEKQQKKKSYKNSGTIRVKICRVETEYSFLDYVMG GCQINFTVGVDFTGSNGDPSSPDSLHYLSPTGVNEYLMALWSVGSVVQDYDSDKLFPAFGFGAQVPPDWQ VSHEFALNFNPSNPYCAGIQGIVDAYRQALPQVRLYGPTNFAPIINHVARFAAQAAHQGTASQYFMLLLL TDGAVTDVEATREAVVRASNLPMSVIIVGVGGADFEAMEQLDADGGPLHTRSGQAAARDIVQFVPYRRFQ NAPREALAQTVLAEVPTQLVSYFRAQGWAPLKPLPPSAKDPAQAPQA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_152928 |
ORF Size | 1611 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_152928.3 |
RefSeq Size | 2049 bp |
RefSeq ORF | 1614 bp |
Locus ID | 8904 |
UniProt ID | Q99829 |
Cytogenetics | 20q11.22 |
Gene Summary | Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene encodes a calcium-dependent protein that also contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. However, the encoded protein does not contain a predicted signal sequence or transmembrane domains. This protein has a broad tissue distribution and it may function in membrane trafficking. This gene and the gene for RNA binding motif protein 12 overlap at map location 20q11.21. Alternate splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Aug 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212736 | CPNE1 (Myc-DDK-tagged)-Human copine I (CPNE1), transcript variant 5 |
USD 500.00 |
|
RC212736L3 | Lenti-ORF clone of CPNE1 (Myc-DDK-tagged)-Human copine I (CPNE1), transcript variant 5 |
USD 800.00 |
|
RC212736L4 | Lenti-ORF clone of CPNE1 (mGFP-tagged)-Human copine I (CPNE1), transcript variant 5 |
USD 800.00 |
|
SC124580 | CPNE1 (untagged)-Human copine I (CPNE1), transcript variant 5 |
USD 501.00 |
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