SURF5 (MED22) (NM_133640) Human Tagged ORF Clone
CAT#: RG212017
- TrueORF®
MED22 (tGFP-tagged) - Human mediator complex subunit 22 (MED22), transcript variant b
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_133640" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | SURF5 |
Synonyms | MED24; SRB6; surf-5; SURF5 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG212017 representing NM_133640
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGCAGAGAGCCCTGCCCCAGAGCAAGGAGACGCTGCTGCAGTCCTACAACAAGCGGCTGAAGG ACGACATTAAGTCCATCATGGACAACTTCACCGAGATCATCAAGACCGCCAAGATTGAGGACGAGACGCA GGTGTCACGGGCCACTCAGGGTGAACAGGACAATTACGAGATGCATGTGCGAGCCGCCAACATCGTCCGA GCCGGCGAGTCCCTGATGAAGCTGGTGTCCGACCTCAAGCAGTTCCTGATCCTCAATGACTTCCCCTCCG TGAACGAGGCCATTGACCAGCGCAACCAGCAGCTGCGCACACTGCAGGAGGAGTGCGACCGGAAGCTCAT CACGCTGCGAGACGAGATCTCCATTGACCTCTACGAGCTGGAGGAGGAGTATTACTCGTCCAGCTCAAGC CTTTGCGAAGCTAATGACCTGCCTCTGTGCGAAGCTTACGGGAGGCTGGACCTCGACACAGACTCTGCTG ATGGCCTCTCGGCCCCTCTGCTGGCGTCCCCGGAGCCCAGTGCTGGCCCCCTACAGGTGGCAGCCCCTGC CCACTCCCATGCTGGTGGCCCTGGCCCCACTGAGCACGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212017 representing NM_133640
Red=Cloning site Green=Tags(s) MAQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEMHVRAANIVR AGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRTLQEECDRKLITLRDEISIDLYELEEEYYSSSSS LCEANDLPLCEAYGRLDLDTDSADGLSAPLLASPEPSAGPLQVAAPAHSHAGGPGPTEHA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_133640 |
ORF Size | 600 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133640.5 |
RefSeq Size | 1448 bp |
RefSeq ORF | 603 bp |
Locus ID | 6837 |
UniProt ID | Q15528 |
Cytogenetics | 9q34.2 |
Gene Summary | This gene encodes a protein component of the mediator complex, which functions in the regulation of transcription by bridging interactions between gene-specific regulatory factors, RNA polymerase II, and general transcription factors. Alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212017 | MED22 (Myc-DDK-tagged)-Human mediator complex subunit 22 (MED22), transcript variant b |
USD 480.00 |
|
RC212017L3 | Lenti-ORF clone of MED22 (Myc-DDK-tagged)-Human mediator complex subunit 22 (MED22), transcript variant b |
USD 780.00 |
|
RC212017L4 | Lenti-ORF clone of MED22 (mGFP-tagged)-Human mediator complex subunit 22 (MED22), transcript variant b |
USD 780.00 |
|
SC111029 | MED22 (untagged)-Human mediator complex subunit 22 (MED22), transcript variant b |
USD 450.00 |
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