PCDHB7 (NM_018940) Human Tagged ORF Clone

CAT#: RG211592

PCDHB7 (tGFP-tagged) - Human protocadherin beta 7 (PCDHB7)

Product Images

Circular map for RG211592

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PCDHB7
• Synonyms: PCDH-BETA7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211592 representing NM_018940
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCAGAGTGGAGCGTGCTGTGCAGAAAAGGCAAGTCTTATTTCTTTGTGTATTTCTGGGAATGT
  CTTGGGCTGGCGCCGAACCGCTTCGGTATTTTGTGGCGGAGGAAACCGAGAGAGGCACCTTTCTTACCAA
  CTTGGCAAAAGACCTAGGGTTAGGGGTAGGGGAACTGAGAGCCCGGGGAACTAGAATTGTTTCAGACCAG
  AACATGCAAATTTTACTGCTCAGTTCGCTTACTGGTGATCTACTTCTAAATGAGAAATTGGACCGAGAGG
  AACTGTGTGGCCCCAGAGAGCCCTGTGTGCTGCCTTTCCAGTTGTTATTGGAAAAACCTTTTCAGATTTT
  CCGTGCTGAACTATGGGTCAGAGACATCAATGATCACGCTCCAGTATTTCTAGACAGAGAGATTTCCTTG
  AAAATATTAGAAAGTACCACTCCAGGGGCGGCATTTCTCCTAGAGAGTGCACAGGATTCAGATGTTGGAA
  CCAACAGCCTGAGTAACTACACCATCAGCCCCAATGCCTATTTCCATATTAATGTCCATGATAGCGGGGA
  GGGGAATATCTATCCCGAATTGGTGCTGAATCAAGTGCTGGATCGGGAAGAGATACCAGAGTTCAGTTTA
  ACCCTCACCGCTTTAGACGGCGGCTCTCCTCCAAGATCAGGGACCGCCCTCGTGCGCATTCTGGTTCTAG
  ACGTAAATGACAACGCCCCTGATTTTGTGCGGTCGCTCTACAAGGTGCAGGTGCCCGAAAATAGCCCCGT
  TGGTTCCATGGTTGTCTCCGTGTCAGCCAGAGATTTAGATACCGGAAGTAATGGGGAAATAGCCTATGCA
  TTTTCTTACGCCACTGAAAGAATTCTCAAAACGTTTCAAATCAATCCAACATCTGGCAGTCTTCATCTTA
  AAGCGCAATTGGACTATGAGGCAATTCAAACTTACACATTAACTATTCAGGCCAAAGACGGCGGCGGGCT
  TTCTGGAAAATGCACTGTAGTGGTTGATGTAACAGATATAAACGATAATCGACCCGAGCTGCTCCTGTCT
  TCACTTACTAGCCCAATTGCAGAAAACTCACCCGAGACAGTCGTGGCTGTTTTTAGGATTAGAGACAGAG
  ATTCCGGGAACAATGGAAAGACAGTGTGCTCCATCCAGGACGATGTCCCCTTCATCCTGAAGCCATCTGT
  CGAAAACTTCTATACTCTGGTAACAGAGAAACCTTTGGATCGAGAGAGGAACACTGAGTACAACATCACC
  ATCACCGTCACCGACTTGGGGACACCCAGGCTGAAAACCGAGCACAACATAACCGTGCTGGTCTCCGACG
  TCAATGACAACGCTCCCGCCTTCACCCAAACCTCCTACACCCTGTTTGTCCGTGAGAACAACAGCCCCGC
  CCTGCCCATCGGCAGTGTCAGCGCCACAGACAGAGACTCGGGCACCAACGCCCAGGTCATCTACTCCCTG
  CTGCCGTCCCAGGACCCGCACCTGCCCCTCGCCTCCCTGGTCTCCATCAACGCGGACAACGGCCACCTGT
  TTGCCCTCAGGTCCCTGGACTACGAGGCCCTGCAGGCGTTCGAGTTCCGCGTGGGCGCCACAGACCGCGG
  CTCCCCCGCGCTGAGCAGCGAGGCGCTGGTGCGCGTGCTGGTGCTGGACGCCAACGACAACTCGCCCTTC
  GTGCTGTACCCGCTGCAGAACAGCTCCGCGCCCTGCACCGAGCCGTTGCCCCGGGCGGCCGAGCCGGGCT
  ACCTGGTGACCAAGGTGGTGGCGGTGGACGGCGACTCGGGCCAGAACGCCTGGCTGTCGTACCAGCTGCT
  CAAGGCCACGGAGCCCGGGCTATTCGGCGTGTGGGCGCACAATGGCGAGGTGCGTACCGCCAGGCTGCTG
  AGCGAGCGCGACGCAGCCAAGCAGAGGCTGGTGGTGCTGGTCAAGGACAATGGCGAGCCTCCGCGCTCGG
  CCACCGCCACGCTGCACGTGCTCCTGGTGGACGGCTTCTCCCAGCCCTACCTGCGGCTCCCGGAGGCGGC
  CCCGGACCAGGCCAACTCGCTCACCGTCTACCTGGTGGTGGCGTTGGCCTCGGTGTCTTCGCTCTTCCTC
  CTCTCGGTGCTCCTGTTCGTGGCGGTGCGGCTGTGCAGGAGGAGCAGGGCGGCCCCGGTGGGTCGCTGCT
  CGGTGCCTGAGGGCCCCTTTCCACGACATCTGGTGGACTTGAGCGGCACCGGGACCCTATCCCAGAGCTA
  CCAGTATGAGGTGTGCCTGACTGGAGGCTCCGGGACAAATGAGTTCAAGTTTCTGAAACCAATTATCCCC
  AACCTGCTACCCCAGAGCACAGGCAGGGAAGTGGAAGAAAATCGCCCATTTCAGAATAATTTGGGTTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211592 representing NM_018940
  Red=Cloning site Green=Tags(s)
  
  MEARVERAVQKRQVLFLCVFLGMSWAGAEPLRYFVAEETERGTFLTNLAKDLGLGVGELRARGTRIVSDQ
  NMQILLLSSLTGDLLLNEKLDREELCGPREPCVLPFQLLLEKPFQIFRAELWVRDINDHAPVFLDREISL
  KILESTTPGAAFLLESAQDSDVGTNSLSNYTISPNAYFHINVHDSGEGNIYPELVLNQVLDREEIPEFSL
  TLTALDGGSPPRSGTALVRILVLDVNDNAPDFVRSLYKVQVPENSPVGSMVVSVSARDLDTGSNGEIAYA
  FSYATERILKTFQINPTSGSLHLKAQLDYEAIQTYTLTIQAKDGGGLSGKCTVVVDVTDINDNRPELLLS
  SLTSPIAENSPETVVAVFRIRDRDSGNNGKTVCSIQDDVPFILKPSVENFYTLVTEKPLDRERNTEYNIT
  ITVTDLGTPRLKTEHNITVLVSDVNDNAPAFTQTSYTLFVRENNSPALPIGSVSATDRDSGTNAQVIYSL
  LPSQDPHLPLASLVSINADNGHLFALRSLDYEALQAFEFRVGATDRGSPALSSEALVRVLVLDANDNSPF
  VLYPLQNSSAPCTEPLPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFGVWAHNGEVRTARLL
  SERDAAKQRLVVLVKDNGEPPRSATATLHVLLVDGFSQPYLRLPEAAPDQANSLTVYLVVALASVSSLFL
  LSVLLFVAVRLCRRSRAAPVGRCSVPEGPFPRHLVDLSGTGTLSQSYQYEVCLTGGSGTNEFKFLKPIIP
  NLLPQSTGREVEENRPFQNNLGF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018940
• ORF Size: 2379 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018940.4
• RefSeq Size: 3715 bp
• RefSeq ORF: 2382 bp
• Locus ID: 56129
• UniProt ID: Q9Y5E2
• Cytogenetics: 5q31.3
• Protein Families: Transmembrane
• Gene Summary: This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections. The transcript for this particular family member uses more than one polyadenylation site. [provided by RefSeq, Jul 2008]