Dermokine (DMKN) (NM_001035516) Human Tagged ORF Clone
CAT#: RG211509
- TrueORF®
DMKN (tGFP-tagged) - Human dermokine (DMKN), transcript variant 1
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001035516" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Dermokine |
Synonyms | UNQ729; ZD52F10 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG211509 representing NM_001035516
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACATGAAGCCGGCCACTGCCTCTGCTCTGCTCCTGCTCCTGCTGGGCCTGGCCTGGACCCAGGGGA GCCACGGCTGGGGTGCGGACGCGTCATCACTGCAGAAACGTGCAGGCAGAGCCGATCAGAACTACAATTA CAACCAGCATGCGTATCCCACTGCCTATGGTGGGAAGTACTCAGTCAAGACCCCTGCAAAGGGGGGAGTC TCACCTTCTTCCTCGGCTTCCCGGGTGCAACCTGGCCTGCTGCAGTGGGTGAAGTTTTGG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG211509 representing NM_001035516
Red=Cloning site Green=Tags(s) MNMKPATASALLLLLLGLAWTQGSHGWGADASSLQKRAGRADQNYNYNQHAYPTAYGGKYSVKTPAKGGV SPSSSASRVQPGLLQWVKFW SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001035516 |
ORF Size | 270 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001035516.3, NP_001030593.1 |
RefSeq Size | 613 bp |
RefSeq ORF | 273 bp |
Locus ID | 93099 |
Cytogenetics | 19q13.12 |
Gene Summary | This gene is upregulated in inflammatory diseases, and it was first observed as expressed in the differentiated layers of skin. The most interesting aspect of this gene is the differential use of promoters and terminators to generate isoforms with unique cellular distributions and domain components. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC211509 | DMKN (Myc-DDK-tagged)-Human dermokine (DMKN), transcript variant 1 |
USD 165.00 |
|
RC211509L3 | Lenti-ORF clone of DMKN (Myc-DDK-tagged)-Human dermokine (DMKN), transcript variant 1 |
USD 465.00 |
|
RC211509L4 | Lenti-ORF clone of DMKN (mGFP-tagged)-Human dermokine (DMKN), transcript variant 1 |
USD 465.00 |
|
SC302840 | DMKN (untagged)-Human dermokine (DMKN), transcript variant 1 |
USD 165.00 |
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