KCNH5 (NM_172375) Human Tagged ORF Clone

CAT#: RG211497

KCNH5 (tGFP-tagged) - Human potassium voltage-gated channel, subfamily H (eag-related), member 5 (KCNH5), transcript variant 3

Product Images

Circular map for RG211497

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KCNH5
• Synonyms: EAG2; H-EAG2; hEAG2; Kv10.2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211497 representing NM_172375
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGGGGGCAAGAGAGGGCTGGTGGCACCGCAGAACACATTTTTGGAGAACATCGTCAGGCGCTCCA
  GTGAATCAAGTTTCTTACTGGGAAATGCCCAGATTGTGGATTGGCCTGTAGTTTATAGTAATGACGGTTT
  TTGTAAACTCTCTGGATATCATCGAGCTGACGTCATGCAGAAAAGCAGCACTTGCAGTTTTATGTATGGG
  GAATTGACTGACAAGAAGACCATTGAGAAAGTCAGGCAAACTTTTGACAACTACGAATCAAACTGCTTTG
  AAGTTCTTCTGTACAAGAAAAACAGAACCCCTGTTTGGTTTTATATGCAAATTGCACCAATAAGAAATGA
  ACATGAAAAGGTGGTCTTGTTCCTGTGTACTTTCAAGGATATTACGTTGTTCAAACAGCCAATAGAGGAT
  GATTCAACAAAAGGTTGGACGAAATTTGCCCGATTGACACGGGCTTTGACAAATAGCCGAAGTGTTTTGC
  AGCAGCTCACGCCAATGAATAAAACAGAGGTGGTCCATAAACATTCAAGACTAGCTGAAGTTCTTCAGCT
  GGGATCAGATATCCTTCCTCAGTATAAACAAGAAGCGCCAAAGACGCCACCACACATTATTTTACATTAT
  TGTGCTTTTAAAACTACTTGGGATTGGGTGATTTTAATTCTTACCTTCTACACCGCCATTATGGTTCCTT
  ATAATGTTTCCTTCAAAACAAAGCAGAACAACATAGCCTGGCTGGTACTGGATAGTGTGGTGGACGTTAT
  TTTTCTGGTTGACATCGTTTTAAATTTTCACACGACTTTCGTGGGGCCCGGTGGAGAGGTCATTTCTGAC
  CCTAAGCTCATAAGGATGAACTATCTGAAAACTTGGTTTGTGATCGATCTGCTGTCTTGTTTACCTTATG
  ACATCATCAATGCCTTTGAAAATGTGGATGAGGGAATCAGCAGTCTCTTCAGTTCTTTAAAAGTGGTGCG
  TCTCTTACGACTGGGCCGTGTGGCTAGGAAACTGGACCATTACCTAGAATATGGAGCAGCAGTCCTCGTG
  CTCCTGGTGTGTGTGTTTGGACTGGTGGCCCACTGGCTGGCCTGCATATGGTATAGCATCGGAGACTACG
  AGGTCATTGATGAAGTCACTAACACCATCCAAATAGACAGTTGGCTCTACCAGCTGGCTTTGAGCATTGG
  GACTCCATATCGCTACAATACCAGTGCTGGGATATGGGAAGGAGGACCCAGCAAGGATTCATTGTACGTG
  TCCTCTCTCTACTTTACCATGACAAGCCTTACAACCATAGGATTTGGAAACATAGCTCCTACCACAGATG
  TGGAGAAGATGTTTTCGGTGGCTATGATGATGGTTGGCTCTCTTCTTTATGCAACTATTTTTGGAAATGT
  TACAACAATTTTCCAGCAAATGTATGCCAACACCAACCGATACCATGAGATGCTGAATAATGTACGGGAC
  TTCCTAAAACTCTATCAGGTCCCAAAAGGCCTTAGTGAGCGAGTCATGGATTATATTGTCTCAACATGGT
  CCATGTCAAAAGGCATTGATACAGAAAAGGTCCTCTCCATCTGTCCCAAGGACATGAGAGCTGATATCTG
  TGTTCATCTAAACCGGAAGGTTTTTAATGAACATCCTGCTTTTCGATTGGCCAGCGATGGGTGTCTGCGC
  GCCTTGGCGGTAGAGTTCCAAACCATTCACTGTGCTCCCGGGGACCTCATTTACCATGCTGGAGAAAGTG
  TGGATGCCCTCTGCTTTGTGGTGTCAGGATCCTTGGAAGTCATCCAGGATGATGAGGTGGTGGCTATTTT
  AGATCATCTTTCG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211497 representing NM_172375
  Red=Cloning site Green=Tags(s)
  
  MPGGKRGLVAPQNTFLENIVRRSSESSFLLGNAQIVDWPVVYSNDGFCKLSGYHRADVMQKSSTCSFMYG
  ELTDKKTIEKVRQTFDNYESNCFEVLLYKKNRTPVWFYMQIAPIRNEHEKVVLFLCTFKDITLFKQPIED
  DSTKGWTKFARLTRALTNSRSVLQQLTPMNKTEVVHKHSRLAEVLQLGSDILPQYKQEAPKTPPHIILHY
  CAFKTTWDWVILILTFYTAIMVPYNVSFKTKQNNIAWLVLDSVVDVIFLVDIVLNFHTTFVGPGGEVISD
  PKLIRMNYLKTWFVIDLLSCLPYDIINAFENVDEGISSLFSSLKVVRLLRLGRVARKLDHYLEYGAAVLV
  LLVCVFGLVAHWLACIWYSIGDYEVIDEVTNTIQIDSWLYQLALSIGTPYRYNTSAGIWEGGPSKDSLYV
  SSLYFTMTSLTTIGFGNIAPTTDVEKMFSVAMMMVGSLLYATIFGNVTTIFQQMYANTNRYHEMLNNVRD
  FLKLYQVPKGLSERVMDYIVSTWSMSKGIDTEKVLSICPKDMRADICVHLNRKVFNEHPAFRLASDGCLR
  ALAVEFQTIHCAPGDLIYHAGESVDALCFVVSGSLEVIQDDEVVAILDHLS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172375
• ORF Size: 1833 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172375.3
• RefSeq Size: 3118 bp
• RefSeq ORF: 1836 bp
• Locus ID: 27133
• UniProt ID: Q8NCM2
• Cytogenetics: 14q23.2
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: This gene encodes a member of voltage-gated potassium channels. Members of this family have diverse functions, including regulating neurotransmitter and hormone release, cardiac function, and cell volume. This protein is an outward-rectifying, noninactivating channel. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]