ARID5B (NM_032199) Human Tagged ORF Clone
CAT#: RG211454
- TrueORF®
ARID5B (tGFP-tagged) - Human AT rich interactive domain 5B (MRF1-like) (ARID5B)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_032199" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | ARID5B |
Synonyms | DESRT; MRF-2; MRF2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG211454 representing NM_032199
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCAACTCACTCCAGTGGGTCGGCTCACCGTGTGGCTTGCACGGACCTTACATTTTCTACAAGG CTTTTCAATTCCACCTTGAAGGCAAACCAAGAATTTTGTCCCTTGGCGACTTTTTCTTTGTAAGATGTAC GCCAAAGGATCCGATTTGCATAGCGGAGCTCCAGCTGTTGTGGGAAGAGAGGACCAGCCGGCAACTTTTA TCCAGCTCTAAACTTTATTTCCTCCCAGAAGACACTCCCCAGGGCAGAAATAGCGACCATGGCGAGGATG AAGTCATTGCTGTTTCCGAAAAGGTGATTGTGAAGCTTGAAGACCTGGTCAAGTGGGTACATTCTGATTT CTCCAAGTGGAGATGTGGCTTCCACGCTGGACCAGTGAAAACTGAGGCCTTGGGAAGGAATGGACAGAAG GAAGCTCTGCTGAAGTACAGGCAGTCAACCCTAAACAGTGGACTCAACTTCAAAGACGTTCTCAAGGAGA AGGCAGACCTGGGGGAGGACGAGGAAGAAACGAACGTGATAGTTCTCAGCTACCCCCAGTACTGCCGGTA CCGCTCGATGCTGAAACGCATCCAGGATAAGCCATCTTCCATTCTAACGGACCAGTTTGCATTGGCCCTG GGGGGCATTGCAGTGGTCAGCAGGAACCCTCAGATCCTGTACTGTCGGGACACCTTTGACCACCCGACTC TCATAGAAAACGAGAGTATATGCGATGAGTTTGCGCCAAATCTTAAAGGCAGACCACGCAAAAAGAAACC ATGCCCACAAAGAAGAGATTCATTCAGTGGTGTTAAGGATTCCAACAACAATTCCGATGGCAAAGCCGTT GCCAAGGTGAAATGTGAGGCCAGGTCAGCCTTGACCAAGCCGAAGAATAACCATAACTGTAAAAAAGTCT CAAATGAAGAAAAACCAAAGGTTGCCATTGGTGAAGAGTGCAGGGCAGATGAACAAGCCTTCTTGGTGGC ACTTTATAAATACATGAAAGAAAGGAAAACGCCGATAGAACGAATACCCTATTTAGGTTTTAAACAGATT AACCTTTGGACTATGTTTCAAGCTGCTCAAAAACTGGGAGGATATGAAACAATAACAGCCCGCCGTCAGT GGAAACATATTTATGATGAATTAGGCGGTAATCCTGGGAGCACCAGCGCTGCCACTTGTACCCGCAGACA TTATGAAAGATTAATCCTACCATATGAAAGATTTATTAAAGGAGAAGAAGATAAGCCCCTGCCTCCAATC AAACCTCGGAAACAGGAGAACAGTTCACAGGAAAATGAGAACAAAACAAAAGTATCTGGAACCAAACGCA TCAAACATGAAATACCTAAAAGCAAGAAAGAAAAAGAAAATGCCCCAAAGCCCCAGGATGCAGCAGAGGT TTCATCAGAGCAAGAAAAAGAACAAGAGACTTTAATAAGCCAGAAAAGCATCCCTGAGCCTCTCCCAGCA GCAGACATGAAGAAAAAAATAGAAGGGTATCAGGAATTTTCAGCGAAGCCCCTGGCATCCAGAGTAGACC CAGAGAAGGACAACGAAACAGACCAAGGTTCCAACAGTGAGAAGGTGGCAGAGGAGGCGGGAGAGAAGGG GCCCACACCTCCACTCCCAAGTGCTCCTCTGGCCCCAGAAAAAGATTCAGCCTTGGTCCCTGGGGCCAGC AAACAGCCACTCACCTCTCCTAGTGCCCTGGTGGACTCAAAACAAGAATCCAAACTGTGCTGTTTTACAG AGAGCCCTGAAAGTGAACCCCAAGAAGCATCCTTCCCCAGCTTCCCCACCACACAGCCACCGCTGGCAAA CCAGAATGAGACGGAGGATGACAAACTGCCCGCCATGGCAGATTACATTGCCAACTGCACCGTGAAGGTG GACCAGCTGGGCAGTGACGACATCCACAATGCGCTCAAGCAGACCCCAAAGGTCCTTGTGGTCCAGTCGT TTGACATGTTCAAAGACAAAGACCTGACTGGGCCCATGAACGAGAACCATGGACTTAATTACACGCCCCT GCTCTACTCTAGGGGCAACCCAGGCATCATGTCCCCACTGGCCAAGAAAAAGCTTTTGTCCCAAGTGAGT GGGGCCAGCCTCTCCAGCAGCTACCCTTATGGCTCCCCACCCCCTTTGATCAGCAAAAAGAAACTGATTG CTAGGGATGACTTGTGTTCCAGTTTGTCCCAGACCCACCATGGCCAAAGCACTGACCATATGGCGGTCAG CCGGCCATCAGTGATTCAGCACGTCCAGAGTTTCAGAAGCAAGCCCTCGGAAGAGAGAAAGACCATCAAT GACATCTTTAAGCATGAGAAACTGAGTCGATCAGATCCCCACCGCTGCAGCTTCTCCAAGCATCACCTTA ACCCCCTTGCTGACTCCTACGTCCTGAAGCAAGAAATTCAGGAGGGCAAGGATAAACTCTTAGAGAAAAG GGCCCTCCCCCATTCCCACATGCCTAGCTTCCTGGCTGACTTCTACTCGTCCCCTCATCTCCATAGCCTC TACAGACACACCGAGCACCATCTTCATAATGAACAGACATCCAAATACCCTTCCAGGGACATGTACAGGG AATCGGAAAACAGTTCTTTTCCTTCCCACAGACACCAAGAAAAGCTCCATGTAAATTATCTCACGTCCCT GCACCTGCAAGACAAAAAGTCGGCGGCAGCAGAAGCCCCTACGGATGATCAGCCTACAGATCTGAGCCTT CCCAAGAACCCGCACAAACCTACCGGCAAGGTCCTGGGCCTGGCTCATTCCACCACAGGGCCCCAGGAGA GCAAAGGCATCTCCCAGTTCCAGGTCTTAGGCAGCCAGAGTCGAGACTGTCACCCCAAAGCCTGTCGGGT ATCACCCATGACCATGTCAGGCCCTAAAAAATACCCTGAATCGCTTTCAAGATCAGGAAAACCTCACCAT GTGAGACTGGAGAATTTCAGGAAGATGGAAGGCATGGTCCACCCAATCCTGCACCGGAAAATGAGCCCGC AGAACATTGGGGCGGCGCGGCCGATCAAGCGCAGCCTGGAGGATTTGGACCTTGTGATTGCAGGGAAAAA GGCCCGGGCAGTGTCTCCCTTAGACCCATCCAAGGAGGTCTCTGGGAAGGAGAAGGCCTCTGAGCAGGAG AGTGAAGGCAGCAAAGCAGCGCACGGTGGGCATTCCGGGGGCGGATCAGAAGGCCACAAGCTTCCCCTCT CCTCCCCTATCTTCCCAGGTCTGTATTCCGGGAGCCTGTGTAACTCGGGCCTCAACTCCAGGCTCCCGGC TGGGTATTCTCATTCTCTGCAGTACTTGAAAAACCAGACTGTGCTTTCTCCACTCATGCAGCCCCTGGCT TTCCACTCGCTTGTGATGCAAAGAGGAATTTTTACATCACCGACAAATTCTCAGCAGCTGTACAGACACT TGGCTGCGGCTACACCTGTAGGAAGTTCATATGGGGACCTTTTGCATAACAGCATTTACCCTTTAGCTGC TATAAATCCTCAAGCTGCCTTTCCATCTTCCCAGCTGTCATCCGTGCACCCCAGTACAAAACTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG211454 representing NM_032199
Red=Cloning site Green=Tags(s) MEPNSLQWVGSPCGLHGPYIFYKAFQFHLEGKPRILSLGDFFFVRCTPKDPICIAELQLLWEERTSRQLL SSSKLYFLPEDTPQGRNSDHGEDEVIAVSEKVIVKLEDLVKWVHSDFSKWRCGFHAGPVKTEALGRNGQK EALLKYRQSTLNSGLNFKDVLKEKADLGEDEEETNVIVLSYPQYCRYRSMLKRIQDKPSSILTDQFALAL GGIAVVSRNPQILYCRDTFDHPTLIENESICDEFAPNLKGRPRKKKPCPQRRDSFSGVKDSNNNSDGKAV AKVKCEARSALTKPKNNHNCKKVSNEEKPKVAIGEECRADEQAFLVALYKYMKERKTPIERIPYLGFKQI NLWTMFQAAQKLGGYETITARRQWKHIYDELGGNPGSTSAATCTRRHYERLILPYERFIKGEEDKPLPPI KPRKQENSSQENENKTKVSGTKRIKHEIPKSKKEKENAPKPQDAAEVSSEQEKEQETLISQKSIPEPLPA ADMKKKIEGYQEFSAKPLASRVDPEKDNETDQGSNSEKVAEEAGEKGPTPPLPSAPLAPEKDSALVPGAS KQPLTSPSALVDSKQESKLCCFTESPESEPQEASFPSFPTTQPPLANQNETEDDKLPAMADYIANCTVKV DQLGSDDIHNALKQTPKVLVVQSFDMFKDKDLTGPMNENHGLNYTPLLYSRGNPGIMSPLAKKKLLSQVS GASLSSSYPYGSPPPLISKKKLIARDDLCSSLSQTHHGQSTDHMAVSRPSVIQHVQSFRSKPSEERKTIN DIFKHEKLSRSDPHRCSFSKHHLNPLADSYVLKQEIQEGKDKLLEKRALPHSHMPSFLADFYSSPHLHSL YRHTEHHLHNEQTSKYPSRDMYRESENSSFPSHRHQEKLHVNYLTSLHLQDKKSAAAEAPTDDQPTDLSL PKNPHKPTGKVLGLAHSTTGPQESKGISQFQVLGSQSRDCHPKACRVSPMTMSGPKKYPESLSRSGKPHH VRLENFRKMEGMVHPILHRKMSPQNIGAARPIKRSLEDLDLVIAGKKARAVSPLDPSKEVSGKEKASEQE SEGSKAAHGGHSGGGSEGHKLPLSSPIFPGLYSGSLCNSGLNSRLPAGYSHSLQYLKNQTVLSPLMQPLA FHSLVMQRGIFTSPTNSQQLYRHLAAATPVGSSYGDLLHNSIYPLAAINPQAAFPSSQLSSVHPSTKL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_032199 |
ORF Size | 3564 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_032199.3 |
RefSeq Size | 7507 bp |
RefSeq ORF | 3567 bp |
Locus ID | 84159 |
UniProt ID | Q14865 |
Cytogenetics | 10q21.2 |
Gene Summary | This gene encodes a member of the AT-rich interaction domain (ARID) family of DNA binding proteins. The encoded protein forms a histone H3K9Me2 demethylase complex with PHD finger protein 2 and regulates the transcription of target genes involved in adipogenesis and liver development. This gene also plays a role in cell growth and differentiation of B-lymphocyte progenitors, and single nucleotide polymorphisms in this gene are associated with acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC211454 | ARID5B (Myc-DDK-tagged)-Human AT rich interactive domain 5B (MRF1-like) (ARID5B) |
USD 1,034.00 |
|
RC211454L3 | Lenti ORF clone of Human AT rich interactive domain 5B (MRF1-like) (ARID5B), Myc-DDK-tagged |
USD 1,334.00 |
|
RC211454L4 | Lenti ORF clone of Human AT rich interactive domain 5B (MRF1-like) (ARID5B), mGFP tagged |
USD 1,334.00 |
|
SC305411 | ARID5B (untagged)-Human AT rich interactive domain 5B (MRF1-like) (ARID5B) |
USD 1,138.00 |
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