Attractin (ATRN) (NM_139322) Human Tagged ORF Clone

CAT#: RG210990

ATRN (tGFP-tagged) - Human attractin (ATRN), transcript variant 2

Product Images

Circular map for RG210990

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Attractin
• Synonyms: DPPT-L; MGCA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210990 representing NM_139322
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCTGCAGCGGCGGCAACTGAGGCAAGGCTGAGGAGGAGGACGGCGGCGACGGCAGCGCTCGCGG
  GCAGGAGCGGCGGGCCGCACTGGGACTGGGACGTGACCAGGGCTGGGAGGCCGGGGCTGGGGGCCGGGCT
  GCGCCTCCCGCGGCTGCTGTCTCCACCGCTGCGGCCACGGCTGCTGCTGCTGCTGTTGTTGCTCTCGCCG
  CCGCTGCTGCTGCTGCTGCTGCCCTGTGAGGCCGAGGCCGCCGCGGCGGCGGCGGCGGTGTCGGGCTCAG
  CCGCAGCCGAGGCCAAGGAATGTGACCGGCCCTGTGTCAACGGCGGTCGCTGCAACCCTGGCACCGGCCA
  GTGCGTCTGCCCCGCCGGCTGGGTGGGCGAGCAATGCCAGCACTGCGGGGGCCGCTTCAGACTAACTGGA
  TCTTCTGGGTTTGTGACAGATGGACCTGGAAATTATAAATACAAAACGAAGTGCACGTGGCTCATTGAAG
  GACAGCCAAATAGAATAATGAGACTTCGTTTCAATCATTTTGCTACAGAGTGTAGTTGGGACCATTTATA
  TGTTTATGATGGGGACTCAATTTATGCACCGCTAGTTGCTGCATTTAGTGGCCTCATTGTTCCTGAGAGA
  GATGGCAATGAGACTGTCCCTGAGGTTGTTGCCACATCAGGTTATGCCTTGCTGCATTTTTTTAGTGATG
  CTGCTTATAATTTGACTGGATTTAATATTACTTACAGTTTTGATATGTGTCCAAATAACTGCTCAGGCCG
  AGGAGAGTGTAAGATCAGTAATAGCAGCGATACTGTTGAATGTGAATGTTCTGAAAACTGGAAAGGTGAA
  GCATGTGACATTCCTCACTGTACAGACAACTGTGGTTTTCCTCATCGAGGCATCTGCAATTCAAGTGATG
  TCAGAGGATGCTCCTGCTTCTCAGACTGGCAGGGTCCTGGATGTTCAGTTCCTGTACCAGCTAACCAGTC
  ATTTTGGACTCGAGAGGAATATTCTAACTTAAAGCTCCCCAGAGCATCTCATAAAGCTGTGGTCAATGGA
  AACATTATGTGGGTTGTTGGAGGATATATGTTCAACCACTCAGATTATAACATGGTTCTAGCGTATGACC
  TTGCTTCTAGGGAGTGGCTTCCACTAAACCGTTCTGTGAACAATGTGGTTGTTAGATATGGTCATTCTTT
  GGCATTATACAAGGATAAAATTTACATGTATGGAGGAAAAATTGATTCAACTGGGAATGTGACCAATGAG
  TTGAGAGTTTTTCACATTCATAATGAGTCATGGGTGTTGTTGACCCCTAAGGCAAAGGAGCAGTATGCAG
  TGGTTGGGCACTCTGCACACATTGTTACACTGAAGAATGGCCGAGTGGTCATGCTGGTCATCTTTGGTCA
  CTGCCCTCTCTATGGATATATAAGCAATGTGCAGGAATATGATTTGGATAAGAACACATGGAGTATATTA
  CACACCCAGGGTGCCCTTGTGCAAGGGGGTTACGGCCATAGCAGTGTTTACGACCATAGGACCAGGGCCC
  TATACGTTCATGGTGGCTACAAGGCTTTCAGTGCCAATAAGTACCGGCTTGCAGATGATCTCTACCGATA
  TGATGTGGATACCCAGATGTGGACCATTCTTAAGGACAGCCGATTTTTCCGTTACTTGCACACAGCTGTG
  ATAGTGAGTGGAACCATGCTGGTGTTTGGAGGAAACACACACAATGACACATCTATGAGCCATGGCGCCA
  AATGCTTCTCTTCAGATTTCATGGCCTATGACATTGCCTGTGACCGCTGGTCAGTGCTTCCCAGACCTGA
  TCTCCACCATGATGTCAACAGATTTGGCCATTCAGCAGTCTTACACAACAGCACCATGTATGTGTTCGGT
  GGTTTCAATAGTCTCCTCCTCAGCGACATCCTGGTATTCACCTCGGAACAGTGTGATGCGCATCGGAGTG
  AAGCCGCTTGTTTAGCAGCAGGACCTGGTATTCGGTGTGTGTGGAACACAGGGTCGTCTCAGTGTATCTC
  GTGGGCGCTGGCAACTGATGAACAAGAAGAAAAGTTAAAATCAGAATGTTTTTCCAAAAGAACTCTTGAC
  CATGACAGATGTGACCAGCACACAGATTGTTACAGCTGCACAGCCAACACCAATGACTGCCACTGGTGCA
  ATGACCATTGTGTCCCCAGGAACCACAGCTGCTCAGAAGGCCAGATCTCCATTTTTAGGTATGAGAATTG
  CCCCAAGGATAACCCCATGTACTACTGTAACAAGAAGACCAGCTGCAGGAGCTGTGCCCTGGACCAGAAC
  TGCCAGTGGGAGCCCCGGAATCAGGAGTGCATTGCCCTGCCCGAAAATATCTGTGGCATTGGCTGGCATT
  TGGTTGGAAACTCATGTTTGAAAATTACTACTGCCAAGGAGAATTATGACAATGCTAAATTGTTCTGTAG
  GAACCACAATGCCCTTTTGGCTTCTCTTACAACCCAGAAGAAGGTAGAATTTGTCCTTAAGCAGCTGCGA
  ATAATGCAGTCATCTCAGAGCATGTCCAAGCTCACCTTAACCCCATGGGTCGGCCTTCGGAAGATCAATG
  TGTCCTACTGGTGCTGGGAAGATATGTCCCCATTTACAAATAGTTTACTACAGTGGATGCCGTCTGAGCC
  CAGTGATGCTGGATTCTGTGGAATTTTATCAGAACCCAGTACTCGGGGACTGAAGGCTGCAACCTGCATC
  AACCCACTCAATGGTAGTGTCTGTGAAAGGCCTGCAAACCACAGTGCTAAGCAGTGCCGGACACCATGTG
  CCTTGAGGACAGCATGTGGAGATTGCACCAGCGGCAGCTCTGAGTGCATGTGGTGCAGCAACATGAAGCA
  GTGTGTGGACTCCAATGCCTATGTGGCCTCCTTCCCTTTTGGCCAGTGTATGGAATGGTATACGATGAGC
  ACCTGCCCCCCTGAAAATTGTTCAGGCTACTGTACCTGTAGTCATTGCTTGGAGCAACCAGGCTGTGGCT
  GGTGTACTGATCCCAGCAATACTGGCAAAGGGAAATGCATAGAGGGTTCCTATAAAGGACCAGTGAAGAT
  GCCTTCGCAAGCCCCTACAGGAAATTTCTATCCACAGCCCCTGCTCAATTCCAGCATGTGTCTAGAGGAC
  AGCAGATACAACTGGTCTTTCATTCACTGTCCAGCTTGCCAATGCAACGGCCACAGTAAATGCATCAATC
  AGAGCATCTGTGAGAAGTGTGAGAACCTGACCACAGGCAAGCACTGCGAGACCTGCATATCTGGCTTCTA
  CGGTGATCCCACCAATGGAGGGAAATGTCAGCCATGCAAGTGCAATGGGCACGCGTCTCTGTGCAACACC
  AACACGGGCAAGTGCTTCTGCACCACCAAGGGCGTCAAGGGGGACGAGTGCCAGCTATGTGAGGTAGAAA
  ATCGATACCAAGGAAACCCTCTCAGAGGAACATGTTATTATACTCTTCTTATTGACTATCAGTTCACCTT
  TAGTCTATCCCAGGAAGATGATCGCTATTACACAGCTATCAATTTTGTGGCTACTCCTGACGAACAAAAC
  AGGGATTTGGACATGTTCATCAATGCCTCCAAGAATTTCAACCTCAACATCACCTGGGCTGCCAGTTTCT
  CAGCTGGAACCCAGGCTGGAGAAGAGATGCCTGTTGTTTCAAAAACCAACATTAAGGAGTACAAAGATAG
  TTTCTCTAATGAGAAGTTTGATTTTCGCAACCACCCAAATATCACTTTCTTTGTTTATGTCAGTAATTTC
  ACCTGGCCCATCAAAATTCAGGTGCAAACTGAACAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210990 representing NM_139322
  Red=Cloning site Green=Tags(s)
  
  MVAAAAATEARLRRRTAATAALAGRSGGPHWDWDVTRAGRPGLGAGLRLPRLLSPPLRPRLLLLLLLLSP
  PLLLLLLPCEAEAAAAAAAVSGSAAAEAKECDRPCVNGGRCNPGTGQCVCPAGWVGEQCQHCGGRFRLTG
  SSGFVTDGPGNYKYKTKCTWLIEGQPNRIMRLRFNHFATECSWDHLYVYDGDSIYAPLVAAFSGLIVPER
  DGNETVPEVVATSGYALLHFFSDAAYNLTGFNITYSFDMCPNNCSGRGECKISNSSDTVECECSENWKGE
  ACDIPHCTDNCGFPHRGICNSSDVRGCSCFSDWQGPGCSVPVPANQSFWTREEYSNLKLPRASHKAVVNG
  NIMWVVGGYMFNHSDYNMVLAYDLASREWLPLNRSVNNVVVRYGHSLALYKDKIYMYGGKIDSTGNVTNE
  LRVFHIHNESWVLLTPKAKEQYAVVGHSAHIVTLKNGRVVMLVIFGHCPLYGYISNVQEYDLDKNTWSIL
  HTQGALVQGGYGHSSVYDHRTRALYVHGGYKAFSANKYRLADDLYRYDVDTQMWTILKDSRFFRYLHTAV
  IVSGTMLVFGGNTHNDTSMSHGAKCFSSDFMAYDIACDRWSVLPRPDLHHDVNRFGHSAVLHNSTMYVFG
  GFNSLLLSDILVFTSEQCDAHRSEAACLAAGPGIRCVWNTGSSQCISWALATDEQEEKLKSECFSKRTLD
  HDRCDQHTDCYSCTANTNDCHWCNDHCVPRNHSCSEGQISIFRYENCPKDNPMYYCNKKTSCRSCALDQN
  CQWEPRNQECIALPENICGIGWHLVGNSCLKITTAKENYDNAKLFCRNHNALLASLTTQKKVEFVLKQLR
  IMQSSQSMSKLTLTPWVGLRKINVSYWCWEDMSPFTNSLLQWMPSEPSDAGFCGILSEPSTRGLKAATCI
  NPLNGSVCERPANHSAKQCRTPCALRTACGDCTSGSSECMWCSNMKQCVDSNAYVASFPFGQCMEWYTMS
  TCPPENCSGYCTCSHCLEQPGCGWCTDPSNTGKGKCIEGSYKGPVKMPSQAPTGNFYPQPLLNSSMCLED
  SRYNWSFIHCPACQCNGHSKCINQSICEKCENLTTGKHCETCISGFYGDPTNGGKCQPCKCNGHASLCNT
  NTGKCFCTTKGVKGDECQLCEVENRYQGNPLRGTCYYTLLIDYQFTFSLSQEDDRYYTAINFVATPDEQN
  RDLDMFINASKNFNLNITWAASFSAGTQAGEEMPVVSKTNIKEYKDSFSNEKFDFRNHPNITFFVYVSNF
  TWPIKIQVQTEQ
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139322
• ORF Size: 3816 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139322.1, NP_647538.1
• RefSeq Size: 4116 bp
• RefSeq ORF: 3819 bp
• Locus ID: 8455
• UniProt ID: O75882
• Cytogenetics: 20p13
• Domains: CUB, CLECT, EGF_Lam, PSI, PSI, EGF, Kelch
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Gene Summary: This gene encodes both membrane-bound and secreted protein isoforms. A membrane-bound isoform exhibits sequence similarity with the mouse mahogany protein, a receptor involved in controlling obesity. A secreted isoform is involved in the initial immune cell clustering during inflammatory responses that may regulate the chemotactic activity of chemokines. [provided by RefSeq, Apr 2016]