LILRA1 (NM_006863) Human Tagged ORF Clone

CAT#: RG210808

LILRA1 (tGFP-tagged) - Human leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 1 (LILRA1)

Product Images

Circular map for RG210808

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LILRA1
• Synonyms: CD85I; LIR-6; LIR6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210808 representing NM_006863
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCCCATCGTCACAGTCCTGATCTGTCTCAGGCTGAGTCTGGGCCCCCGGACCCACGTGCAGGCAG
  GGACCCTCCCCAAGCCCACACTCTGGGCTGAGCCAGGCTCTGTGATCACCCAGGGGAGTCCCGTGACCCT
  CTGGTGTCAGGGGATCCTGGAGACCCAGGAGTACCGTCTGTATAGAGAAAAGAAAACAGCACCCTGGATT
  ACACGGATCCCACAGGAGATTGTGAAGAAGGGCCAGTTCCCCATCCCATCCATCACCTGGGAACACACAG
  GGCGGTATCGCTGTTTCTACGGTAGCCACACTGCAGGCTGGTCAGAGCCCAGTGACCCCCTGGAGCTGGT
  GGTGACAGGAGCCTACATCAAACCCACCCTCTCAGCTCTACCCAGCCCTGTGGTGACCTCAGGAGGGAAC
  GTGACCCTCCATTGTGTCTCACAGGTGGCATTTGGCAGCTTCATTCTGTGTAAGGAAGGAGAAGATGAAC
  ACCCACAATGCCTGAACTCACAGCCCCGTACCCATGGGTGGTCCCGGGCCATCTTCTCTGTGGGCCCCGT
  GAGCCCGAGTCGCAGGTGGTCGTACAGGTGCTATGCTTATGACTCGAACTCTCCCCATGTGTGGTCTCTA
  CCCAGTGATCTCCTGGAGCTCCTGGTCCTAGGTGTTTCTAAGAAGCCATCACTCTCAGTGCAGCCAGGTC
  CTATAGTGGCCCCTGGGGAGAGCCTGACCCTCCAGTGTGTTTCTGATGTCAGCTACGACAGATTTGTTCT
  GTATAAGGAGGGAGAACGTGACTTCCTCCAGCTCCCTGGCCCACAGCCCCAGGCTGGGCTCTCCCAGGCC
  AACTTCACCCTGGGCCCTGTGAGCCGCTCCTACGGGGGCCAGTACAGATGCTCCGGTGCATACAACCTCT
  CCTCCGAGTGGTCGGCCCCCAGCGACCCCCTGGACATCCTGATCGCAGGACAGTTCCGTGGCAGACCCTT
  CATCTCGGTGCATCCGGGCCCCACGGTGGCCTCAGGAGAGAACGTGACCCTGCTGTGTCAGTCATGGGGG
  CCGTTCCACACTTTCCTTCTGACCAAGGCGGGAGCAGCTGATGCCCCCCTCCGTCTCAGATCAATACACG
  AATATCCTAAGTACCAGGCTGAATTCCCTATGAGTCCTGTGACCTCAGCCCACTCGGGGACCTACAGGTG
  CTACGGCTCACTCAGCTCCAACCCCTACCTGCTGTCTCACCCCAGTGACTCCCTGGAGCTCATGGTCTCA
  GGAGCAGCTGAGACCCTCAGCCCACCACAAAACAAGTCCGATTCCAAGGCTGGAGCAGCTAACACCCTCA
  GCCCATCACAAAACAAGACTGCCTCACACCCCCAGGATTACACAGTGGAGAATCTCATCCGCATGGGCAT
  AGCTGGCTTGGTCCTGGTGGTCCTCGGGATTCTGCTATTTGAGGCTCAGCACAGCCAGAGAAGCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210808 representing NM_006863
  Red=Cloning site Green=Tags(s)
  
  MTPIVTVLICLRLSLGPRTHVQAGTLPKPTLWAEPGSVITQGSPVTLWCQGILETQEYRLYREKKTAPWI
  TRIPQEIVKKGQFPIPSITWEHTGRYRCFYGSHTAGWSEPSDPLELVVTGAYIKPTLSALPSPVVTSGGN
  VTLHCVSQVAFGSFILCKEGEDEHPQCLNSQPRTHGWSRAIFSVGPVSPSRRWSYRCYAYDSNSPHVWSL
  PSDLLELLVLGVSKKPSLSVQPGPIVAPGESLTLQCVSDVSYDRFVLYKEGERDFLQLPGPQPQAGLSQA
  NFTLGPVSRSYGGQYRCSGAYNLSSEWSAPSDPLDILIAGQFRGRPFISVHPGPTVASGENVTLLCQSWG
  PFHTFLLTKAGAADAPLRLRSIHEYPKYQAEFPMSPVTSAHSGTYRCYGSLSSNPYLLSHPSDSLELMVS
  GAAETLSPPQNKSDSKAGAANTLSPSQNKTASHPQDYTVENLIRMGIAGLVLVVLGILLFEAQHSQRSL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006863
• ORF Size: 1467 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006863.4
• RefSeq Size: 1910 bp
• RefSeq ORF: 1470 bp
• Locus ID: 11024
• UniProt ID: O75019
• Cytogenetics: 19q13.42
• Gene Summary: This gene encodes an activating member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein is predominantly expressed in B cells, interacts with major histocompatibility complex class I ligands, and contributes to the regulation of immune responses. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]