MAP7D1 (NM_018067) Human Tagged ORF Clone

CAT#: RG210725

MAP7D1 (tGFP-tagged) - Human MAP7 domain containing 1 (MAP7D1)

Product Images

Circular map for RG210725

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MAP7D1
• Synonyms: PARCC1; RPRC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210725 representing NM_018067
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCGGCCCGCGTGCGGAGCTGGGGGCGGGCGCACCCCCAGCTGTGGTCGCCAGGACCCCCCCAG
  AGCCAAGACCTTCTCCAGAAGGTGACCCTTCCCCCCCACCACCACCAATGTCAGCCCTGGTCCCCGACAC
  TCCCCCGGACACCCCTCCTGCCATGAAGAATGCCACTAGCTCTAAGCAGCTCCCACTGGAACCAGAGAGC
  CCCTCAGGGCAGGTCGGGCCTAGGCCAGCCCCCCCGCAGGAAGAGTCCCCTTCCTCTGAAGCAAAGAGCA
  GAGGACCCACCCCACCAGCCATGGGCCCACGGGATGCCAGACCTCCTCGAAGGAGCAGCCAGCCATCTCC
  AACAGCAGTGCCAGCCTCCGACAGCCCTCCCACCAAGCAAGAGGTGAAGAAGGCAGGAGAGAGACACAAG
  CTGGCAAAGGAGCGGCGAGAAGAGCGGGCCAAGTACCTGGCGGCCAAGAAGGCAGTGTGGCTGGAGAAGG
  AGGAGAAGGCCAAGGCGCTGCGGGAGAAGCAGCTCCAGGAGCGCCGGCGCCGGCTGGAGGAGCAACGTCT
  TAAAGCCGAGCAACGCCGTGCAGCCCTGGAGGAACGGCAGCGGCAGAAGCTCGAGAAAAACAAGGAGCGC
  TATGAAGCAGCCATCCAACGGTCAGTGAAGAAGACGTGGGCCGAAATCCGGCAGCAGCGCTGGTCCTGGG
  CAGGGGCCCTGCACCACAGCTCTCCAGGACATAAGACCAGTGGGAGCAGGTGCTCCGTGTCGGCAGTTAA
  CCTGCCCAAACACGTGGACTCTATAATCAACAAGCGGCTCTCAAAGTCCTCTGCCACGCTCTGGAACTCC
  CCCAGTAGAAATCGCAGCCTGCAGCTGAGCGCATGGGAGAGCAGCATCGTGGATCGTCTGATGACGCCCA
  CTCTCTCCTTCCTTGCTCGGAGTCGCAGCGCGGTCACACTGCCCCGCAACGGCCGGGACCAGGGTAGGGG
  CTGCGACCCTGGGAGAGGCCCCACGTGGGGCCGGGCAGGGGCCAGCCTGGCGCGCGGGCCGCAACCCGAC
  CGCACTCATCCCTCTGCAGCCGTGCCGGTGTGCCCGCGCTCGGCCTCCGCCAGCCCCCTGACGCCGTGCA
  GCGTCACCCGAAGCGTGCACCGCTGCGCCCCCGCCGGTGAGCGCGGGGAGCGCCGCAAGCCCAACGCCGG
  GGGCAGCCCCGCTCCGGTGCGCCGCCGGCCGGAGGCCTCGCCGGTGCAGAAAAAGGAGAAGAAGGACAAG
  GAGCGGGAAAACGAGAAGGAGAAGAGTGCCCTAGCCCGGGAGCGCAGCCTCAAGAAGCGCCAGTCGCTGC
  CCGCCTCCCCACGTGCCCGCCTCTCCGCCAGCACCGCCTCTGAGCTCAGCCCCAAATCCAAGGCCAGGCC
  ATCCTCTCCCTCCACATCCTGGCACAGGCCTGCCTCCCCCTGCCCCAGCCCAGGGCCAGGCCACACTCTG
  CCTCCAAAGCCACCGTCCCCCCGAGGCACCACTGCATCCCCCAAGGGGCGGGTTCGGAGGAAGGAGGAGG
  CAAAGGAGAGCCCCAGCGCCGCAGGGCCCGAGGACAAGAGCCAGAGCAAGCGCAGGGCCAGTAACGAGAA
  GGAGTCAGCAGCCCCAGCCTCACCGGCACCTTCGCCGGCGCCCTCGCCCACCCCAGCCCCGCCCCAGAAG
  GAGCAGCCCCCCGCGGAGACCCCTACAGACGCTGCTGTCTTGACCTCACCCCCAGCCCCTGCTCCCCCGG
  TGACCCCTAGCAAACCAATGGCCGGCACCACAGACCGAGAAGAAGCCACTCGGCTCTTGGCTGAGAAGCG
  GCGCCAGGCCCGGGAGCAGCGGGAGCGCGAGGAGCAGGAGCGGAGGCTGCAGGCAGAAAGGGACAAGCGA
  ATGCGAGAGGAGCAGCTGGCACGGGAGGCCGAGGCCCGGGCGGAGCGGGAGGCGGAGGCCCGGAGGCGGG
  AGGAGCAGGAGGCACGAGAGAAGGCGCAGGCCGAGCAGGAGGAGCAGGAGCGGCTGCAGAAGCAGAAAGA
  GGAGGCCGAAGCTCGGTCGCGGGAAGAGGCGGAGCGGCAGCGTCTGGAGCGGGAAAAGCACTTCCAGCAG
  CAGGAGCAAGAGCGGCAAGAGCGCAGAAAGCGTCTGGAGGAGATCATGAAGAGGACTCGGAAGTCAGAAG
  TTTCTGAAACCAAGCAGAAGCAGGACAGCAAGGAGGCCAACGCCAACGGTTCCAGCCCAGAGCCTGTGAA
  AGCTGTGGAGGCTCGGTCCCCAGGGCTGCAGAAGGAGGCTGTGCAGAAAGAGGAGCCCATCCCACAGGAG
  CCTCAGTGGAGTCTCCCAAGCAAGGAGTTGCCAGCGTCCCTGGTGAATGGCCTGCAGCCTCTCCCAGCAC
  ACCAGGAGAATGGCTTCTCCACCAACGGACCCTCTGGGGACAAGAGTCTGAGCCGAACACCAGAGACACT
  CCTGCCCTTTGCAGAGGCAGAAGCCTTCCTCAAGAAAGCTGTGGTGCAGTCCCCGCAGGTCACAGAAGTC
  CTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210725 representing NM_018067
  Red=Cloning site Green=Tags(s)
  
  MESGPRAELGAGAPPAVVARTPPEPRPSPEGDPSPPPPPMSALVPDTPPDTPPAMKNATSSKQLPLEPES
  PSGQVGPRPAPPQEESPSSEAKSRGPTPPAMGPRDARPPRRSSQPSPTAVPASDSPPTKQEVKKAGERHK
  LAKERREERAKYLAAKKAVWLEKEEKAKALREKQLQERRRRLEEQRLKAEQRRAALEERQRQKLEKNKER
  YEAAIQRSVKKTWAEIRQQRWSWAGALHHSSPGHKTSGSRCSVSAVNLPKHVDSIINKRLSKSSATLWNS
  PSRNRSLQLSAWESSIVDRLMTPTLSFLARSRSAVTLPRNGRDQGRGCDPGRGPTWGRAGASLARGPQPD
  RTHPSAAVPVCPRSASASPLTPCSVTRSVHRCAPAGERGERRKPNAGGSPAPVRRRPEASPVQKKEKKDK
  ERENEKEKSALARERSLKKRQSLPASPRARLSASTASELSPKSKARPSSPSTSWHRPASPCPSPGPGHTL
  PPKPPSPRGTTASPKGRVRRKEEAKESPSAAGPEDKSQSKRRASNEKESAAPASPAPSPAPSPTPAPPQK
  EQPPAETPTDAAVLTSPPAPAPPVTPSKPMAGTTDREEATRLLAEKRRQAREQREREEQERRLQAERDKR
  MREEQLAREAEARAEREAEARRREEQEAREKAQAEQEEQERLQKQKEEAEARSREEAERQRLEREKHFQQ
  QEQERQERRKRLEEIMKRTRKSEVSETKQKQDSKEANANGSSPEPVKAVEARSPGLQKEAVQKEEPIPQE
  PQWSLPSKELPASLVNGLQPLPAHQENGFSTNGPSGDKSLSRTPETLLPFAEAEAFLKKAVVQSPQVTEV
  L
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018067
• ORF Size: 2523 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018067.3, NP_060537.3
• RefSeq Size: 3357 bp
• RefSeq ORF: 2526 bp
• Locus ID: 55700
• UniProt ID: Q3KQU3
• Cytogenetics: 1p34.3