CRSP7 (MED26) (NM_004831) Human Tagged ORF Clone

CAT#: RG210714

MED26 (tGFP-tagged) - Human mediator complex subunit 26 (MED26)

Product Images

Circular map for RG210714

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CRSP7
• Synonyms: CRSP7; CRSP70
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210714 representing NM_004831
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCGGCTCCGGCGTCTCCGCAGCAGATCAGGGACCGGCTGCTGCAGGCCATCGACCCCCAGAGCA
  ACATCCGGAACATGGTGGCGGTGCTGGAAGTCATCTCCAGCCTGGAGAAATACCCTATTACCAAAGAGGC
  ACTTGAGGAAACACGACTTGGGAAGCTCATCAACGACGTCCGCAAGAAAACCAAGAACGAGGAGCTCGCC
  AAGCGGGCCAAGAAGCTGCTGCGGAGCTGGCAGAAGCTCATCGAGCCGGCACACCAGCATGAGGCGGCGC
  TGCGGGGGCTGGCGGGGGCCACCGGCTCTGCCAACGGGGGCGCACACAACTGCCGGCCGGAGGTGGGGGC
  GGCTGGCCCACCCAGGAGCATCCATGACCTGAAGAGCCGCAATGACCTCCAGAGGCTGCCCGGGCAGCGG
  CTGGACAGGCTGGGCAGCCGCAAGCGCCGGGGTGACCAGCGTGACCTCGGCCACCCAGGGCCGCCACCCA
  AGGTCTCCAAAGCTAGCCACGACCCCCTGGTCCCCAACTCATCCCCCCTCCCCACCAACGGGATCAGTGG
  GAGTCCAGAGAGCTTCGCCAGCTCCCTGGATGGCAGTGGGCATGCAGGCCCAGAGGGCAGCCGCCTGGAG
  CGTGACGAGAATGACAAGCACAGTGGCAAGATCCCCGTCAACGCCGTGCGACCGCACACCAGCTCCCCGG
  GCCTGGGCAAGCCCCCTGGACCCTGCTTGCAGCCAAAGGCTTCGGTGCTGCAGCAGCTGGACAGGGTGGA
  CGAGACTCCGGGGCCTCCCCATCCCAAGGGACCCCCTCGCTGCTCTTTCAGTCCTCGGAACTCACGGCAT
  GAGGGCTCCTTTGCCCGGCAGCAGAGCTTGTATGCACCCAAGGGCTCCGTGCCCAGCCCCTCACCGCGGC
  CCCAGGCACTCGATGCCACACAGGTGCCGTCACCGCTTCCACTGGCACAGCCGTCCACACCCCCCGTACG
  GCGGCTCGAGCTGCTGCCCAGTGCGGAAAGCCCAGTGTGCTGGCTTGAGCAGCCTGAGAGCCACCAGCGG
  CTGGCGGGGCCGGGCTGCAAGGCAGGGCTGTCCCCAGCCGAGCCCCTCCTGTCCCGGGCAGGCTTTTCCC
  CAGACTCCTCCAAGGCGGACAGTGATGCTGCCTCCTCAGGGGGCTCGGACAGTAAAAAGAAGAAGAGGTA
  CCGACCTCGAGACTATACGGTTAACTTGGACGGGCAGGTGGCTGAGGCGGGCGTCAAGCCTGTCCGGTTA
  AAAGAGCGGAAGCTCACCTTTGACCCCATGACGAGACAGATCAAACCTCTGACCCAGAAAGAGCCAGTGC
  GGGCAGACAGCCCTGTGCACATGGAGCAGCAGTCCAGGACAGAGCTGGACAAGCAGGAGGCCAAGGCCAG
  CCTCCAGAGCCCCTTCGAACAGACGAACTGGAAGGAGCTGTCACGCAACGAGATCATCCAGTCCTACCTG
  AGCCGGCAGAGCAGCCTGCTCTCATCATCGGGCGCGCAGACCCCAGGGGCTCACCACTTCATGTCTGAGT
  ACCTGAAGCAGGAGGAGAGCACCCGGCAAGGGGCCAGGCAGCTGCATGTGCTGGTGCCTCAAAGCCCGCC
  CACGGACCTCCCTGGTCTGACCCGGGAGGTCACACAGGACGATCTCGACAGAATCCAGGCCAGCCAGTGG
  CCGGGGGTGAACGGGTGTCAGGACACACAGGGTAACTGGTATGACTGGACGCAGTGCATATCGCTCGATC
  CGCACGGCGACGACGGGCGCTTGAACATTCTGCCTTATGTCTGCTTGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210714 representing NM_004831
  Red=Cloning site Green=Tags(s)
  
  MTAAPASPQQIRDRLLQAIDPQSNIRNMVAVLEVISSLEKYPITKEALEETRLGKLINDVRKKTKNEELA
  KRAKKLLRSWQKLIEPAHQHEAALRGLAGATGSANGGAHNCRPEVGAAGPPRSIHDLKSRNDLQRLPGQR
  LDRLGSRKRRGDQRDLGHPGPPPKVSKASHDPLVPNSSPLPTNGISGSPESFASSLDGSGHAGPEGSRLE
  RDENDKHSGKIPVNAVRPHTSSPGLGKPPGPCLQPKASVLQQLDRVDETPGPPHPKGPPRCSFSPRNSRH
  EGSFARQQSLYAPKGSVPSPSPRPQALDATQVPSPLPLAQPSTPPVRRLELLPSAESPVCWLEQPESHQR
  LAGPGCKAGLSPAEPLLSRAGFSPDSSKADSDAASSGGSDSKKKKRYRPRDYTVNLDGQVAEAGVKPVRL
  KERKLTFDPMTRQIKPLTQKEPVRADSPVHMEQQSRTELDKQEAKASLQSPFEQTNWKELSRNEIIQSYL
  SRQSSLLSSSGAQTPGAHHFMSEYLKQEESTRQGARQLHVLVPQSPPTDLPGLTREVTQDDLDRIQASQW
  PGVNGCQDTQGNWYDWTQCISLDPHGDDGRLNILPYVCLD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004831
• ORF Size: 1800 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004831.5
• RefSeq Size: 3184 bp
• RefSeq ORF: 1803 bp
• Locus ID: 9441
• UniProt ID: O95402
• Cytogenetics: 19p13.11
• Protein Families: Druggable Genome
• Gene Summary: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq, Jul 2008]