GART (NM_000819) Human Tagged ORF Clone

CAT#: RG210447

GART (tGFP-tagged) - Human phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (GART), transcript variant 1

Product Images

Circular map for RG210447

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GART
• Synonyms: AIRS; GARS; GARTF; PAIS; PGFT; PRGS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210447 representing NM_000819
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCCGAGTACTTATAATTGGCAGTGGAGGAAGGGAACATACGCTGGCCTGGAAACTTGCACAGT
  CTCATCATGTCAAACAAGTGTTGGTTGCCCCAGGAAACGCAGGCACTGCCTGCTCTGAAAAGATTTCAAA
  TACCGCCATCTCAATCAGTGACCACACTGCCCTTGCTCAATTCTGCAAAGAGAAGAAAATTGAATTTGTA
  GTTGTTGGACCAGAAGCACCTCTGGCTGCTGGGATTGTTGGGAACCTGAGGTCTGCAGGAGTGCAATGCT
  TTGGCCCAACAGCAGAAGCGGCTCAGTTAGAGTCCAGCAAAAGGTTTGCCAAAGAGTTTATGGACAGACA
  TGGAATCCCAACCGCACAATGGAAGGCTTTCACCAAACCTGAAGAAGCCTGCAGCTTCATTTTGAGTGCA
  GACTTCCCTGCTTTGGTTGTGAAGGCCAGTGGTCTTGCAGCTGGAAAAGGGGTGATTGTTGCAAAGAGCA
  AAGAAGAGGCCTGCAAAGCTGTACAAGAGATCATGCAGGAGAAAGCCTTTGGGGCAGCTGGAGAAACAAT
  TGTCATTGAAGAACTTCTTGACGGAGAAGAGGTGTCGTGTCTGTGTTTCACTGATGGCAAGACTGTGGCC
  CCCATGCCCCCAGCACAGGACCATAAGCGATTACTGGAGGGAGATGGTGGCCCTAACACAGGGGGAATGG
  GAGCCTATTGTCCAGCCCCTCAGGTTTCTAATGATCTATTACTAAAAATTAAAGATACTGTTCTTCAGAG
  GACAGTGGATGGCATGCAGCAAGAGGGTACTCCATATACAGGTATTCTCTATGCTGGAATAATGCTGACC
  AAGAATGGCCCAAAAGTTCTAGAGTTTAATTGCCGTTTTGGTGATCCAGAGTGCCAAGTAATCCTCCCAC
  TTCTTAAAAGTGATCTTTATGAAGTGATTCAGTCCACCTTAGATGGACTGCTCTGCACATCTCTGCCTGT
  TTGGCTAGAAAACCACACCGCCCTAACTGTTGTCATGGCAAGTAAAGGTTATCCTGGAGACTACACCAAG
  GGTGTAGAGATAACAGGGTTTCCTGAGGCTCAAGCTCTAGGACTGGAGGTGTTCCATGCAGGCACTGCCC
  TCAAAAATGGCAAAGTAGTAACTCATGGGGGTAGAGTTCTTGCAGTCACAGCCATCCGGGAAAATCTCAT
  ATCAGCCCTTGAGGAAGCCAAGAAAGGACTAGCTGCTATAAAGTTTGAGGGAGCAATTTATAGGAAAGAC
  GTCGGCTTTCGTGCCATAGCTTTCCTCCAGCAGCCCAGGAGTTTGACTTACAAGGAATCTGGAGTAGATA
  TCGCAGCTGGAAATATGCTGGTCAAGAAAATTCAGCCTTTAGCAAAAGCCACTTCCAGATCAGGCTGTAA
  AGTTGATCTTGGAGGTTTTGCTGGTCTTTTTGATTTAAAAGCAGCTGGTTTCAAAGATCCCCTTCTGGCC
  TCTGGAACAGATGGCGTTGGAACTAAACTAAAGATTGCCCAGCTATGCAATAAACATGATACCATTGGTC
  AAGATTTGGTAGCAATGTGTGTTAATGATATTCTGGCACAAGGAGCAGAGCCCCTCTTCTTCCTTGATTA
  CTTTTCCTGTGGAAAACTTGACCTCAGTGTAACTGAAGCTGTTGTTGCTGGAATTGCTAAAGCTTGTGGA
  AAAGCTGGATGTGCTCTCCTTGGAGGTGAAACAGCAGAAATGCCTGACATGTATCCCCCTGGAGAGTATG
  ACCTAGCTGGGTTTGCCGTTGGTGCCATGGAGCGAGATCAGAAACTCCCTCACCTGGAAAGAATCACTGA
  GGGTGATGTTGTTGTTGGAATAGCTTCATCTGGTCTTCATAGCAATGGATTTAGCCTTGTGAGGAAAATC
  GTGGCAAAATCTTCCCTCCAGTACTCCTCTCCAGCACCTGATGGTTGTGGTGACCAGACTTTAGGGGACT
  TACTTCTCACGCCTACCAGAATCTACAGCCATTCACTGTTACCTGTCCTACGTTCAGGACATGTCAAAGC
  CTTTGCCCATATTACTGGTGGAGGATTACTAGAGAACATCCCCAGAGTCCTCCCTGAGAAACTTGGGGTA
  GATTTAGATGCCCAGACCTGGAGGATCCCCAGGGTCTTCTCATGGTTGCAGCAGGAAGGACACCTCTCTG
  AGGAAGAGATGGCCAGAACATTTAACTGTGGGGTTGGCGCTGTCCTTGTGGTATCAAAGGAGCAGACAGA
  GCAGATTCTGAGGGATATCCAGCAGCACAAGGAAGAAGCCTGGGTGATTGGCAGTGTGGTTGCACGAGCT
  GAAGGTTCCCCACGTGTGAAAGTCAAGAATCTGATTGAAAGCATGCAAATAAATGGGTCAGTGTTGAAGA
  ATGGCTCCCTGACAAATCATTTCTCTTTTGAAAAAAAAAAGGCCAGAGTGGCTGTCTTAATATCTGGAAC
  AGGATCGAACCTGCAAGCACTTATAGACAGTACTCGGGAACCAAATAGCTCTGCACAAATTGATATTGTT
  ATCTCCAACAAAGCCGCAGTAGCTGGGTTAGATAAAGCGGAAAGAGCTGGTATTCCCACTAGAGTAATTA
  ATCATAAACTGTATAAAAATCGTGTAGAATTTGACAGTGCAATTGACCTAGTCCTTGAAGAGTTCTCCAT
  AGACATAGTCTGTCTTGCAGGATTCATGAGAATTCTTTCTGGCCCCTTTGTCCAAAAGTGGAATGGAAAA
  ATGCTCAATATCCACCCATCCTTGCTCCCTTCTTTTAAGGGTTCAAATGCCCATGAGCAAGCCCTGGAAA
  CCGGAGTCACAGTTACTGGGTGCACTGTACACTTTGTAGCTGAAGATGTGGATGCTGGACAGATTATTTT
  GCAAGAAGCTGTTCCCGTGAAGAGGGGTGATACTGTCGCAACTCTTTCTGAAAGAGTAAAATTAGCAGAA
  CATAAAATATTTCCTGCAGCCCTTCAGCTGGTGGCCAGTGGAACTGTACAGCTTGGAGAAAATGGCAAGA
  TCTGTTGGGTTAAAGAGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210447 representing NM_000819
  Red=Cloning site Green=Tags(s)
  
  MAARVLIIGSGGREHTLAWKLAQSHHVKQVLVAPGNAGTACSEKISNTAISISDHTALAQFCKEKKIEFV
  VVGPEAPLAAGIVGNLRSAGVQCFGPTAEAAQLESSKRFAKEFMDRHGIPTAQWKAFTKPEEACSFILSA
  DFPALVVKASGLAAGKGVIVAKSKEEACKAVQEIMQEKAFGAAGETIVIEELLDGEEVSCLCFTDGKTVA
  PMPPAQDHKRLLEGDGGPNTGGMGAYCPAPQVSNDLLLKIKDTVLQRTVDGMQQEGTPYTGILYAGIMLT
  KNGPKVLEFNCRFGDPECQVILPLLKSDLYEVIQSTLDGLLCTSLPVWLENHTALTVVMASKGYPGDYTK
  GVEITGFPEAQALGLEVFHAGTALKNGKVVTHGGRVLAVTAIRENLISALEEAKKGLAAIKFEGAIYRKD
  VGFRAIAFLQQPRSLTYKESGVDIAAGNMLVKKIQPLAKATSRSGCKVDLGGFAGLFDLKAAGFKDPLLA
  SGTDGVGTKLKIAQLCNKHDTIGQDLVAMCVNDILAQGAEPLFFLDYFSCGKLDLSVTEAVVAGIAKACG
  KAGCALLGGETAEMPDMYPPGEYDLAGFAVGAMERDQKLPHLERITEGDVVVGIASSGLHSNGFSLVRKI
  VAKSSLQYSSPAPDGCGDQTLGDLLLTPTRIYSHSLLPVLRSGHVKAFAHITGGGLLENIPRVLPEKLGV
  DLDAQTWRIPRVFSWLQQEGHLSEEEMARTFNCGVGAVLVVSKEQTEQILRDIQQHKEEAWVIGSVVARA
  EGSPRVKVKNLIESMQINGSVLKNGSLTNHFSFEKKKARVAVLISGTGSNLQALIDSTREPNSSAQIDIV
  ISNKAAVAGLDKAERAGIPTRVINHKLYKNRVEFDSAIDLVLEEFSIDIVCLAGFMRILSGPFVQKWNGK
  MLNIHPSLLPSFKGSNAHEQALETGVTVTGCTVHFVAEDVDAGQIILQEAVPVKRGDTVATLSERVKLAE
  HKIFPAALQLVASGTVQLGENGKICWVKEE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000819
• ORF Size: 3030 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000819.5
• RefSeq Size: 3376 bp
• RefSeq ORF: 3033 bp
• Locus ID: 2618
• UniProt ID: P22102
• Cytogenetics: 21q22.11
• Domains: GARS, AIRS, formyl_transf
• Protein Pathways: Metabolic pathways, One carbon pool by folate, Purine metabolism
• Gene Summary: The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]