CLEC18A (NM_182619) Human Tagged ORF Clone

CAT#: RG209903

CLEC18A (tGFP-tagged) - Human C-type lectin domain family 18, member A (CLEC18A), transcript variant 1

Product Images

Circular map for RG209903

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CLEC18A
• Synonyms: MRCL; MRCL1; MRLP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209903 representing NM_182619
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCATCCAGAGACCTCCCCTGGCCGGGGGCATCTCCTGGCTGTGCTCCTGGCCCTCCTTGGCACCG
  CCTGGGCAGAGGTGTGGCCACCCCAGCTGCAGGAGCAGGCTCCGATGGCCGGAGCCCTGAACAGGAAGGA
  GAGTTTCTTGCTCCTCTCCCTGCACAACCGCCTGCGCAGCTGGGTCCAGCCCCCTGCGGCTGACATGCGG
  AGGCTGGACTGGAGTGACAGCCTGGCCCAGCTGGCTCAAGCCAGGGCAGCCCTCTGTGGAACCCCAACCC
  CGAGCCTGGCGTCCGGCCTGTGGCGCACCCTGCAAGTGGGCTGGAACATGCAGCTGCTACCCGCGGGCTT
  GGTGTCCTTTGTCGAAGTGGTCAGCCTATGGTTTGCAGAGGGGCAGCGGTACAGCCACGCGGCAGGAGAG
  TGTGCTCGCAACGCCACCTGCACCCACTACACGCAGCTCGTGTGGGCCACCTCAAGCCAGCTGGGCTGTG
  GGCGGCACCTGTGCTCTGCAGGCCAGGCAGCGATAGAAGCCTTTGTCTGTGCCTACTCCCCCAGAGGCAA
  CTGGGAGGTCAACGGGAAGACAATCGTCCCCTATAAGAAGGGTGCCTGGTGTTCGCTCTGCACAGCCAGT
  GTCTCAGGCTGCTTCAAAGCCTGGGACCATGCAGGGGGGCTCTGTGAGGTCCCCAGGAATCCTTGTCGCA
  TGAGCTGCCAGAACCATGGACGTCTCAACATCAGCACCTGCCACTGCCACTGTCCCCCTGGCTACACGGG
  CAGATACTGCCAAGTGAGGTGCAGCCTGCAGTGTGTGCACGGCCGGTTCCGGGAGGAGGAGTGCTCGTGC
  GTCTGTGACATCGGCTACGGGGGAGCCCAGTGTGCCACCAAGGTGCATTTTCCCTTCCACACCTGTGACC
  TGAGGATCGACGGAGACTGCTTCATGGTGTCTTCAGAGGCAGACACCTATTACAGAGCCAGGATGAAATG
  TCAGAGGAAAGGCGGGGTGCTGGCCCAGATCAAGAGCCAGAAAGTGCAGGACATCCTCGCCTTCTATCTG
  GGCCGCCTGGAGACCACCAACGAGGTGATTGACAGTGACTTCGAGACCAGGAACTTCTGGATCGGGCTCA
  CCTACAAGACCGCCAAGGACTCCTTCCGCTGGGCCACAGGGGAGCACCAGGCCTTCACCAGTTTTGCCTT
  TGGGCAGCCTGACAACCACGGGTTTGGCAACTGCGTGGAGCTGCAGGCTTCAGCTGCCTTCAACTGGAAC
  GACCAGCGCTGCAAAACCCGAAACCGTTACATCTGCCAGTTTGCCCAGGAGCACATCTCCCGGTGGGGCC
  CAGGGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209903 representing NM_182619
  Red=Cloning site Green=Tags(s)
  
  MLHPETSPGRGHLLAVLLALLGTAWAEVWPPQLQEQAPMAGALNRKESFLLLSLHNRLRSWVQPPAADMR
  RLDWSDSLAQLAQARAALCGTPTPSLASGLWRTLQVGWNMQLLPAGLVSFVEVVSLWFAEGQRYSHAAGE
  CARNATCTHYTQLVWATSSQLGCGRHLCSAGQAAIEAFVCAYSPRGNWEVNGKTIVPYKKGAWCSLCTAS
  VSGCFKAWDHAGGLCEVPRNPCRMSCQNHGRLNISTCHCHCPPGYTGRYCQVRCSLQCVHGRFREEECSC
  VCDIGYGGAQCATKVHFPFHTCDLRIDGDCFMVSSEADTYYRARMKCQRKGGVLAQIKSQKVQDILAFYL
  GRLETTNEVIDSDFETRNFWIGLTYKTAKDSFRWATGEHQAFTSFAFGQPDNHGFGNCVELQASAAFNWN
  DQRCKTRNRYICQFAQEHISRWGPGS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182619
• ORF Size: 1338 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182619.4
• RefSeq Size: 2321 bp
• RefSeq ORF: 1341 bp
• Locus ID: 348174
• UniProt ID: A5D8T8
• Cytogenetics: 16q22.1
• Gene Summary: This is one of three closely related paralogous genes on chromosome 16 encoding secreted proteins containing C-type lectin domains. These domains bind to carbohydrates in the presence of calcium, and may be involved in cell adhesion, immune response and apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2012]