NIPAL1 (NM_207330) Human Tagged ORF Clone

CAT#: RG209874

NIPAL1 (tGFP-tagged) - Human NIPA-like domain containing 1 (NIPAL1)

Product Images

Circular map for RG209874

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NIPAL1
• Synonyms: NPAL1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209874 representing NM_207330
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCACAGGTGAGGCTGCCGCCCGGAGAGCCCTGCCGAGAAGGATATGTGCTGTCTCTGGTCTGTC
  CAAACTCCTCCCAGGCTTGGTGTGAGATCACAAATGTGTCACAGCTGCTGGCTTCTCCTGTGCTCTACAC
  GGACCTGAATTACAGCATAAACAACTTGAGCATTTCAGCAAATGTAGAAAACAAATACAGTCTTTATGTG
  GGCTTGGTACTGGCAGTAAGCTCAAGTATTTTTATTGGCTCCAGCTTCATACTGAAAAAGAAGGGCCTCT
  TGCAACTGGCCAGCAAGGGCTTTACTAGAGCTGGACAAGGTGGACATTCTTACCTGAAGGAATGGCTCTG
  GTGGGTAGGATTGCTGTCAATGGGAGCAGGAGAGGCTGCAAATTTTGCTGCTTATGCTTTTGCACCTGCC
  ACCTTGGTCACCCCTCTGGGTGCTTTGAGTGTTCTCATAAGTGCAATATTATCTTCCTACTTTTTAAACG
  AGCACTTGAACATTCATGGGAAAATAGGCTGCATATTAAGTATATTGGGGTCAACTGTGATGGTTATCCA
  TGCCCCACAAGAAGAGGAAGTCACATCTTTGCATGAAATGGAAATGAAATTGAGAGACCCAGGGTTTATT
  TCCTTTGCTGTGATCATAACTGTGATCTCCTTGGTGCTGATTTTGATTGTGGCTCCCAAGAAAGGACAGA
  CCAATATATTGGTTTATATTTCAATCTGTTCCTTGATTGGAGCGTTTTCAGTTTCTTCTGTGAAAGGCCT
  GGGAATTGCCATTAAGGAGCTGATAGAATGGAAGCCAGTTTACAAACATCCGCTGGTCTTTGTTTTGCTG
  GCTGTACTTGTGCTTTCAGTAACTACACAGATTAACTATCTCAACAAGGCACTGGACACCTTTAATACCT
  CTCTTGTGACACCCATTTATTATGTATTCTTCACATCCATGGTAGTGACTTGCTCTGCCATCTTATTCCA
  AGAGTGGTATGGCATGACAGCTGGAGATATCATTGGGACCCTGAGTGGATTCTTCACTATTATCATTGGC
  ATCTTCCTTCTACATGCTTTTAAAAATACTGACATTACTTGGAGTGAGCTTACATCCACTGCTAAGAAAG
  AAGCCGTCTCTCTGAATGTCAATGAAAACAATTATGTTTTACTAGAGAACTTGGAGTGTTCAGCCCCAGG
  ATACAATGATGACGTTACCTTGTTTAGTAGAACTGATGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209874 representing NM_207330
  Red=Cloning site Green=Tags(s)
  
  MGAQVRLPPGEPCREGYVLSLVCPNSSQAWCEITNVSQLLASPVLYTDLNYSINNLSISANVENKYSLYV
  GLVLAVSSSIFIGSSFILKKKGLLQLASKGFTRAGQGGHSYLKEWLWWVGLLSMGAGEAANFAAYAFAPA
  TLVTPLGALSVLISAILSSYFLNEHLNIHGKIGCILSILGSTVMVIHAPQEEEVTSLHEMEMKLRDPGFI
  SFAVIITVISLVLILIVAPKKGQTNILVYISICSLIGAFSVSSVKGLGIAIKELIEWKPVYKHPLVFVLL
  AVLVLSVTTQINYLNKALDTFNTSLVTPIYYVFFTSMVVTCSAILFQEWYGMTAGDIIGTLSGFFTIIIG
  IFLLHAFKNTDITWSELTSTAKKEAVSLNVNENNYVLLENLECSAPGYNDDVTLFSRTDD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207330
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207330.3
• RefSeq Size: 2204 bp
• RefSeq ORF: 1233 bp
• Locus ID: 152519
• UniProt ID: Q6NVV3
• Cytogenetics: 4p12
• Protein Families: Transmembrane
• Gene Summary: Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+), Cu(2+) and Co(2+) but to a much less extent than Mg(2+) (By similarity).[UniProtKB/Swiss-Prot Function]