GPR1 (NM_005279) Human Tagged ORF Clone
CAT#: RG209833
- TrueORF®
GPR1 (tGFP-tagged) - Human G protein-coupled receptor 1 (GPR1), transcript variant 1
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_005279" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | GPR1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG209833 representing NM_005279
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGATTTGGAGGAAACATTATTTGAAGAATTTGAGAACTATTCCTATGACCTAGACTATTACTCTC TGGAGTCTGATTTGGAGGAGAAAGTCCAGCTGGGAGTTGTTCACTGGGTCTCCCTGGTGTTATATTGTTT GGCTTTTGTTCTGGGAATTCCAGGAAATGCCATCGTCATTTGGTTCACGGGGTTCAAGTGGAAGAAGACA GTCACCACTCTGTGGTTCCTCAATCTAGCCATTGCGGATTTCATTTTTCTTCTCTTTCTGCCCCTGTACA TCTCCTATGTGGCCATGAATTTCCACTGGCCCTTTGGCATCTGGCTGTGCAAAGCCAATTCCTTCACTGC CCAGTTGAACATGTTTGCCAGTGTTTTTTTCCTGACAGTGATCAGCCTGGACCACTATATCCACTTGATC CATCCTGTCTTATCTCATCGGCATCGAACCCTCAAGAACTCTCTGATTGTCATTATATTCATCTGGCTTT TGGCTTCTCTAATTGGCGGTCCTGCCCTGTACTTCCGGGATACTGTGGAGTTCAATAATCATACTCTTTG CTATAACAATTTTCAGAAGCATGATCCTGACCTCACTTTGATCAGGCACCATGTTCTGACTTGGGTGAAA TTTATCATTGGCTATCTCTTCCCTTTGCTAACAATGAGTATTTGCTACTTGTGTCTCATCTTCAAGGTGA AGAAGCGAAGCATCCTGATCTCCAGTAGGCATTTCTGGACAATTCTGGTTGTGGTTGTGGCCTTTGTGGT TTGCTGGACTCCTTATCACCTGTTTAGCATTTGGGAGCTCACCATTCACCACAATAGCTATTCCCACCAT GTGATGCAGGCTGGAATCCCCCTCTCCACTGGTTTGGCATTCCTCAATAGTTGCTTGAACCCCATCCTTT ATGTCCTAGTTAGTAAGAAGTTCCAAGCTCGCTTCCGGTCCTCAGTTGCTGAGATACTCAAGTACACACT GTGGGAAGTCAGCTGTTCTGGCACAGTGAGTGAACAGCTCAGGAACTCAGAAACCAAGAATCTGTGTCTC CTGGAAACAGCTCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209833 representing NM_005279
Red=Cloning site Green=Tags(s) MEDLEETLFEEFENYSYDLDYYSLESDLEEKVQLGVVHWVSLVLYCLAFVLGIPGNAIVIWFTGFKWKKT VTTLWFLNLAIADFIFLLFLPLYISYVAMNFHWPFGIWLCKANSFTAQLNMFASVFFLTVISLDHYIHLI HPVLSHRHRTLKNSLIVIIFIWLLASLIGGPALYFRDTVEFNNHTLCYNNFQKHDPDLTLIRHHVLTWVK FIIGYLFPLLTMSICYLCLIFKVKKRSILISSRHFWTILVVVVAFVVCWTPYHLFSIWELTIHHNSYSHH VMQAGIPLSTGLAFLNSCLNPILYVLVSKKFQARFRSSVAEILKYTLWEVSCSGTVSEQLRNSETKNLCL LETAQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_005279 |
ORF Size | 1065 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005279.2, NP_005270.2 |
RefSeq Size | 2089 bp |
RefSeq ORF | 1068 bp |
Locus ID | 2825 |
UniProt ID | P46091 |
Cytogenetics | 2q33.3 |
Domains | 7tm_1 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Gene Summary | Receptor for the inflammation-associated leukocyte chemoattractant chemerin/RARRES2 suggesting a role for this receptor in the regulation of inflammation (PubMed:18165312). Receptor for TAFA1, mediates its effects on neuronal stem-cell proliferation and differentiation via the activation of ROCK/ERK and ROCK/STAT3 signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209833 | GPR1 (Myc-DDK-tagged)-Human G protein-coupled receptor 1 (GPR1), transcript variant 1 |
USD 457.00 |
|
RC209833L1 | Lenti ORF clone of Human G protein-coupled receptor 1 (GPR1), transcript variant 1, Myc-DDK-tagged |
USD 757.00 |
|
RC209833L2 | Lenti ORF clone of Human G protein-coupled receptor 1 (GPR1), transcript variant 1, mGFP tagged |
USD 757.00 |
|
RC209833L3 | Lenti ORF clone of Human G protein-coupled receptor 1 (GPR1), transcript variant 1, Myc-DDK-tagged |
USD 757.00 |
|
RC209833L4 | Lenti ORF clone of Human G protein-coupled receptor 1 (GPR1), transcript variant 1, mGFP tagged |
USD 757.00 |
|
SC116835 | GPR1 (untagged)-Human G protein-coupled receptor 1 (GPR1), transcript variant 1 |
USD 457.00 |
|
SC322087 | GPR1 (untagged)-Human G protein-coupled receptor 1 (GPR1), transcript variant 1 |
USD 457.00 |
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