ZNF224 (NM_013398) Human Tagged ORF Clone

CAT#: RG208733

ZNF224 (tGFP-tagged) - Human zinc finger protein 224 (ZNF224). Note: ORF is codon optimized

Product Images

Circular map for RG208733

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF224
• Synonyms: BMZF-2; BMZF2; KOX22; ZNF27; ZNF233; ZNF255
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208733 ORF sequence, codon optimized.
  Due to the complexity of NM_013398, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACTTTTAAGGAGGCGATGACATTTAAAGACGTCGCTGTGGTTTTTACGGAGGAGGAGCTGGGTC
  TGCTCGATCTCGCCCAGAGGAAATTGTATCGGGATGTGATGTTGGAGAACTTTAGAAATCTGCTGAGTGT
  CGGGCATCAGGCATTTCACCGCGACACCTTCCACTTCCTGAGAGAAGAAAAGATATGGATGATGAAAACG
  GCCATCCAGCGGGAGGGTAACAGCGGGGACAAAATTCAGACAGAAATGGAAACGGTTAGCGAGGCCGGCA
  CACACCAGGAGTGGTCCTTCCAACAAATTTGGGAAAAGATCGCTTCCGATCTTACTCGCTCTCAGGATTT
  GATGATTAATAGCTCCCAGTTCAGTAAAGAGGGTGACTTTCCTTGCCAAACTGAGGCCGGCCTCTCAGTC
  ATCCACACGCGGCAGAAGAGCAGCCAGGGTAATGGCTATAAGCCCAGCTTTAGCGACGTGAGCCATTTCG
  ACTTTCACCAACAGCTGCACTCAGGAGAAAAGAGCCACACCTGCGATGAATGCGGTAAGAATTTTTGCTA
  CATCTCAGCCTTGAGGATTCATCAGCGAGTGCACATGGGCGAGAAGTGTTATAAATGCGACGTGTGCGGA
  AAGGAGTTCAGTCAAAGTAGCCACCTGCAGACCCATCAACGGGTACACACCGGCGAGAAGCCTTTCAAAT
  GCGTGGAATGTGGAAAGGGCTTTAGTCGACGGTCAGCTCTTAATGTCCACCACAAGCTCCATACCGGGGA
  GAAGCCCTATAATTGTGAGGAGTGTGGCAAGGCGTTCATCCACGATTCCCAACTGCAAGAGCATCAGCGC
  ATCCACACAGGTGAAAAGCCTTTCAAATGCGACATTTGTGGGAAGAGTTTCTGTGGCCGCTCACGGCTTA
  ATCGCCACAGCATGGTACATACCGCCGAGAAGCCCTTCCGGTGCGACACCTGCGATAAGTCTTTTCGACA
  GCGGAGCGCTCTGAACTCCCACAGGATGATCCACACCGGAGAGAAGCCATACAAGTGTGAAGAGTGTGGC
  AAGGGGTTCATTTGCCGGCGGGACCTGTATACTCACCATATGGTGCACACCGGCGAGAAGCCTTATAACT
  GTAAAGAGTGCGGTAAGTCCTTCAGGTGGGCCTCCTGCCTGCTGAAGCACCAGAGGGTCCATTCTGGAGA
  GAAACCTTTCAAGTGCGAGGAATGCGGCAAAGGATTTTACACAAACAGTCAGTGCTACAGCCACCAGAGA
  AGCCACAGTGGAGAGAAACCCTACAAGTGCGTGGAATGCGGCAAGGGTTATAAGCGGAGGCTCGATCTCG
  ATTTCCACCAGCGCGTGCACACCGGAGAAAAGCTGTATAATTGTAAAGAATGTGGTAAATCATTTAGCAG
  AGCTCCATGCCTGCTTAAACATGAGCGACTTCACTCAGGCGAAAAGCCCTTCCAGTGTGAAGAATGCGGG
  AAACGGTTCACCCAGAACTCTCACTTGCATTCACACCAAAGGGTACACACCGGAGAGAAACCTTACAAGT
  GTGAGAAATGCGGTAAGGGGTACAACAGCAAATTTAATCTCGATATGCACCAAAAGGTGCATACAGGTGA
  GCGACCGTATAATTGCAAGGAGTGCGGGAAATCTTTTGGATGGGCCTCCTGCCTCCTGAAGCACCAGAGG
  CTTCACTCCGGCGAGAAGCCCTTCAAGTGCGAGGAGTGCGGAAAAAGATTCACCCAGAACTCTCAGCTGC
  ACAGTCACCAGCGAGTCCATACTGGAGAGAAGCCATATAAGTGTGACGAATGCGGAAAGGGATTCTCATG
  GTCCTCTACTCGCCTCACTCATCAACGGCGCCATTCTCGGGAGACACCCCTCAAATGTGAGCAGCATGGC
  AAGAACATCGTCCAAAATTCCTTCAGTAAGGTGCAGGAAAAGGTGCACAGCGTTGAGAAGCCCTATAAAT
  GTGAAGATTGCGGCAAGGGCTACAATAGACGCTTGAACCTCGACATGCATCAGAGAGTTCACATGGGCGA
  GAAAACCTGGAAGTGCCGGGAATGTGACATGTGCTTTTCCCAAGCAAGCAGTCTGCGACTGCACCAGAAC
  GTCCACGTAGGCGAGAAACCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208733 representing NM_013398
  Red=Cloning site Green=Tags(s)
  
  MTTFKEAMTFKDVAVVFTEEELGLLDLAQRKLYRDVMLENFRNLLSVGHQAFHRDTFHFLREEKIWMMKT
  AIQREGNSGDKIQTEMETVSEAGTHQEWSFQQIWEKIASDLTRSQDLMINSSQFSKEGDFPCQTEAGLSV
  IHTRQKSSQGNGYKPSFSDVSHFDFHQQLHSGEKSHTCDECGKNFCYISALRIHQRVHMGEKCYKCDVCG
  KEFSQSSHLQTHQRVHTGEKPFKCVECGKGFSRRSALNVHHKLHTGEKPYNCEECGKAFIHDSQLQEHQR
  IHTGEKPFKCDICGKSFCGRSRLNRHSMVHTAEKPFRCDTCDKSFRQRSALNSHRMIHTGEKPYKCEECG
  KGFICRRDLYTHHMVHTGEKPYNCKECGKSFRWASCLLKHQRVHSGEKPFKCEECGKGFYTNSQCYSHQR
  SHSGEKPYKCVECGKGYKRRLDLDFHQRVHTGEKLYNCKECGKSFSRAPCLLKHERLHSGEKPFQCEECG
  KRFTQNSHLHSHQRVHTGEKPYKCEKCGKGYNSKFNLDMHQKVHTGERPYNCKECGKSFGWASCLLKHQR
  LHSGEKPFKCEECGKRFTQNSQLHSHQRVHTGEKPYKCDECGKGFSWSSTRLTHQRRHSRETPLKCEQHG
  KNIVQNSFSKVQEKVHSVEKPYKCEDCGKGYNRRLNLDMHQRVHMGEKTWKCRECDMCFSQASSLRLHQN
  VHVGEKP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013398
• ORF Size: 2121 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013398.2, NP_037530.2
• RefSeq Size: 3993 bp
• RefSeq ORF: 2124 bp
• Locus ID: 7767
• UniProt ID: Q9NZL3
• Cytogenetics: 19q13.31
• Domains: KRAB, zf-C2H2
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a member of the Krueppel C2H2-type zinc-finger family of proteins. The encoded protein represses transcription of the aldolase A gene, which encodes a key enzyme in glycolysis. The encoded zinc-finger protein may also function as a transcriptional co-activator with Wilms' tumor protein 1 to regulate apoptotic genes in leukemia. [provided by RefSeq, Jul 2016]