DHX8 (NM_004941) Human Tagged ORF Clone
CAT#: RG208403
- TrueORF®
DHX8 (tGFP-tagged) - Human DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8)
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_004941" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | DHX8 |
Synonyms | DDX8; Dhr2; HRH1; PRP22; PRPF22 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG208403 representing NM_004941
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGGCTGTAGCCATGGCGGGAGCCTTAATCGGGTCGGAGCCAGGCCCCGCGGAAGAACTTGCCA AACTCGAGTACCTGTCTTTGGTGTCAAAGGTTTGCACTGAGCTGGACAATCACTTGGGGATCAACGACAA GGACCTTGCTGAATTTGTGATCAGTCTTGCTGAGAAAAATACCACCTTTGATACTTTTAAGGCTTCTCTC GTCAAAAATGGTGCAGAATTTACGGATTCTCTTATTAGTAACTTGCTGCGTCTCATACAAACCATGCGGC CTCCAGCGAAGCCTTCCACTAGCAAAGATCCAGTTGTTAAACCTAAAACAGAAAAAGAAAAGCTGAAGGA ACTCTTCCCAGTCCTTTGCCAACCGGACAACCCTTCTGTTCGGACCATGTTGGATGAAGATGATGTGAAA GTTGCTGTGGATGTCCTGAAAGAACTGGAAGCTTTAATGCCCAGCGCAGCAGGCCAGGAGAAGCAAAGAG ATGCTGAACACCGGGACAGGACAAAGAAGAAGAAGCGGAGTCGAAGCCGAGATCGAAACCGAGATCGAGA CAGAGATAGGGAACGAAACCGAGATAGAGACCACAAGCGGAGACACCGATCCCGCTCTCGATCACGTTCC AGGACCCGGGAGAGGAATAAAGTGAAGTCTAGATATCGGTCCAGGAGCAGGAGTCAGAGTCCCCCCAAAG ACCGGAAGGACCGGGACAAATATGGAGAGCGGAATCTGGATAGATGGCGGGATAAGCATGTGGACCGCCC TCCTCCAGAAGAGCCCACCATTGGTGACATTTATAATGGCAAAGTTACCAGCATCATGCAGTTTGGTTGC TTTGTGCAGCTGGAAGGACTAAGGAAGCGGTGGGAAGGCCTGGTGCACATCTCTGAGCTCCGGCGGGAGG GTCGTGTGGCCAATGTAGCTGATGTCGTGAGCAAAGGCCAGAGGGTCAAAGTCAAAGTGCTGTCCTTCAC TGGGACCAAGACCAGCCTGAGCATGAAGGATGTGGATCAAGAGACTGGAGAAGATCTAAACCCAAATAGA CGGCGAAATCTTGTCGGGGAGACCAATGAGGAGACCTCAATGCGGAATCCTGATAGACCCACTCACTTGT CCCTTGTCAGTGCTCCTGAAGTAGAGGACGACTCACTGGAACGCAAGCGCCTCACCCAAATCTCTGACCC AGAGAAGTGGGAGATCAAACAGATGATTGCTGCCAATGTCCTTTCCAAAGAAGAATTTCCAGACTTTGAT GAAGAGACTGGCATTCTCCCTAAGGTGGATGATGAAGAAGATGAGGACCTTGAGATTGAATTGGTTGAGG AAGAGCCTCCATTCCTGAGAGGGCACACTAAGCAAAGCATGGACATGAGCCCCATTAAAATTGTCAAGAA CCCAGACGGCTCCCTCTCCCAAGCAGCAATGATGCAGAGTGCCTTGGCCAAAGAAAGGCGGGAACTCAAA CAGGCCCAGCGGGAAGCTGAGATGGATTCTATTCCCATGGGACTCAACAAACACTGGGTTGACCCTCTGC CTGATGCGGAAGGCAGACAGATTGCTGCCAACATGAGGGGTATTGGGATGATGCCCAATGATATTCCTGA GTGGAAGAAGCATGCCTTTGGGGGCAACAAAGCCTCTTACGGAAAAAAGACCCAGATGTCAATCCTTGAG CAGAGGGAGAGCCTGCCCATCTACAAACTGAAGGAGCAATTGGTCCAGGCCGTCCATGACAATCAGATCC TGATTGTCATTGGTGAGACAGGATCTGGAAAGACAACACAGATCACCCAGTACCTGGCGGAGGCAGGCTA CACTTCCAGGGGCAAGATTGGGTGTACCCAGCCCAGAAGAGTGGCAGCTATGTCGGTGGCCAAAAGAGTG TCAGAGGAGTTTGGTTGTTGCTTAGGCCAAGAGGTGGGCTACACCATTCGATTTGAGGACTGCACTAGCC CTGAAACAGTCATCAAGTACATGACAGATGGGATGTTGCTTAGAGAGTGCTTGATTGACCCTGACCTCAC TCAGTACGCGATCATCATGTTGGACGAGGCACATGAGAGGACAATTCACACTGATGTGCTCTTTGGATTG TTGAAAAAGACAGTTCAGAAACGGCAGGACATGAAGCTGATTGTCACCTCAGCCACCTTGGATGCAGTGA AGTTTTCTCAATACTTCTATGAAGCTCCCATTTTCACCATCCCAGGTCGAACATATCCAGTGGAAATACT GTACACAAAGGAACCTGAGACAGATTATCTGGATGCCAGCCTGATTACTGTTATGCAGATTCATTTAACA GAACCACCAGGTGATATCCTGGTCTTCCTGACTGGTCAGGAAGAAATTGATACTGCTTGTGAGATCCTGT ATGAAAGAATGAAATCCCTGGGACCTGATGTTCCAGAGTTAATTATCCTCCCAGTGTACTCTGCTCTTCC CAGTGAGATGCAGACCCGAATCTTTGACCCAGCTCCACCAGGCAGCAGAAAGGTTGTGATTGCCACCAAT ATCGCAGAGACATCGCTGACTATTGATGGTATCTACTATGTGGTGGACCCAGGATTCGTGAAACAGAAAG TTTACAATTCCAAGACAGGGATTGACCAGCTCGTGGTGACGCCTATTTCTCAGGCTCAGGCAAAGCAACG AGCTGGCAGAGCTGGGAGAACAGGCCCAGGGAAGTGTTACAGGTTGTACACAGAACGTGCCTACCGAGAT GAAATGCTGACCACCAACGTGCCGGAAATCCAGAGAACCAACTTAGCAAGCACAGTGCTGTCACTCAAGG CCATGGGTATCAATGATCTGCTGTCCTTTGATTTCATGGATGCCCCACCTATGGAAACTTTGATCACAGC CATGGAGCAGCTGTACACACTGGGGGCCCTGGATGACGAGGGCCTGCTCACTCGCTTGGGCCGCCGGATG GCAGAGTTCCCTCTGGAGCCAATGCTATGCAAAATGCTCATCATGTCTGTGCATCTGGGCTGCAGTGAGG AAATGCTGACCATTGTATCCATGCTGTCTGTGCAGAACGTCTTCTATAGGCCCAAGGATAAACAAGCCCT TGCAGATCAGAAGAAGGCCAAATTCCACCAGACTGAAGGGGACCACCTCACCCTGCTAGCTGTGTACAAC TCCTGGAAGAACAACAAGTTCTCCAACCCATGGTGCTATGAGAACTTTATCCAGGCTCGTTCCCTGCGCC GGGCCCAGGACATTCGCGAGCAGATGTTAGGCATAATGGACAGACACAAGCTGGATGTTGTTTCCTGTGG CAAGTCCACAGTCCGAGTGCAGAAGGCCATCTGCAGTGGGTTCTTCCGTAATGCTGCCAAGAAAGACCCG CAGGAGGGTTACCGGACACTGATCGACCAGCAGGTGGTCTATATCCATCCTTCCAGTGCCCTCTTCAACA GACAGCCAGAATGGGTGGTGTACCATGAGCTGGTGCTCACCACCAAGGAATACATGCGTGAAGTTACCAC CATCGACCCTCGGTGGCTTGTGGAGTTTGCCCCAGCCTTCTTCAAGGTCTCAGACCCAACTAAGCTAAGC AAACAGAAGAAGCAACAGCGTCTTGAACCCTTGTACAACCGCTATGAGGAACCCAATGCCTGGAGAATAT CTCGAGCTTTCCGACGGCGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208403 representing NM_004941
Red=Cloning site Green=Tags(s) MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASL VKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVK VAVDVLKELEALMPSAAGQEKQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRS RTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGC FVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNR RRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTQISDPEKWEIKQMIAANVLSKEEFPDFD EETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELK QAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILE QRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRV SEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGL LKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLT EPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATN IAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRD EMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRM AEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYN SWKNNKFSNPWCYENFIQARSLRRAQDIREQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDP QEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLS KQKKQQRLEPLYNRYEEPNAWRISRAFRRR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004941 |
ORF Size | 3660 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004941.1, NP_004932.1 |
RefSeq Size | 4201 bp |
RefSeq ORF | 3663 bp |
Locus ID | 1659 |
UniProt ID | Q14562 |
Cytogenetics | 17q21.31 |
Domains | DEAD, helicase_C, S1, HA2 |
Protein Pathways | Spliceosome |
Gene Summary | This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC208403 | DHX8 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8) |
USD 1,062.00 |
|
RC208403L1 | Lenti ORF clone of Human DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), Myc-DDK-tagged |
USD 1,362.00 |
|
RC208403L2 | Lenti ORF clone of Human DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), mGFP tagged |
USD 1,362.00 |
|
RC208403L3 | Lenti ORF clone of Human DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), Myc-DDK-tagged |
USD 1,362.00 |
|
RC208403L4 | Lenti ORF clone of Human DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), mGFP tagged |
USD 1,362.00 |
|
SC117043 | DHX8 (untagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8) |
USD 1,063.00 |
|
SC324113 | DHX8 (untagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8) |
USD 1,063.00 |
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