CDH12 (NM_004061) Human Tagged ORF Clone

CAT#: RG208156

CDH12 (tGFP-tagged) - Human cadherin 12, type 2 (N-cadherin 2) (CDH12)

Product Images

Circular map for RG208156

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CDH12
• Synonyms: CDHB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208156 representing NM_004061
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTACAAGGAACTGTTTATCCCTGCTTCTCTGGGTTCTGTTTGATGGAGGTCTCCTAACACCACTAC
  AACCACAGCCACAGCAGACTTTAGCCACAGAGCCAAGAGAAAATGTTATCCATCTGCCAGGACAACGGTC
  ACATTTCCAACGTGTTAAACGTGGCTGGGTATGGAATCAATTTTTTGTGCTGGAAGAATACGTGGGCTCC
  GAGCCTCAGTATGTGGGAAAGCTCCATTCCGACTTAGACAAGGGAGAGGGCACTGTGAAATACACCCTCT
  CAGGAGATGGCGCTGGCACCGTTTTTACCATTGATGAAACCACAGGGGACATTCATGCAATAAGGAGCCT
  AGATAGAGAAGAGAAACCTTTCTACACTCTTCGTGCTCAGGCTGTGGACATAGAAACCAGAAAGCCCCTG
  GAGCCTGAATCAGAATTCATCATCAAAGTGCAGGATATTAATGATAATGAGCCAAAGTTTTTGGATGGAC
  CTTATGTTGCTACTGTTCCAGAAATGTCTCCTGTGGGTGCATATGTACTCCAGGTCAAGGCCACAGATGC
  AGATGACCCGACCTATGGAAACAGTGCCAGAGTCGTTTACAGCATTCTTCAGGGACAACCTTATTTCTCT
  ATTGATCCCAAGACAGGTGTTATTAGAACAGCTTTGCCAAACATGGACAGAGAAGTCAAAGAACAATATC
  AAGTACTCATCCAAGCCAAGGATATGGGAGGACAGCTTGGAGGATTAGCCGGAACAACAATAGTCAACAT
  CACTCTCACCGATGTCAATGACAATCCACCTCGATTCCCCAAAAGCATCTTCCACTTGAAAGTTCCTGAG
  TCTTCCCCTATTGGTTCAGCTATTGGAAGAATAAGAGCTGTGGATCCTGATTTTGGACAAAATGCAGAAA
  TTGAATACAATATTGTTCCAGGAGATGGGGGAAATTTGTTTGACATCGTCACAGATGAGGATACACAAGA
  GGGAGTCATCAAATTGAAAAAGCCTTTAGATTTTGAAACAAAGAAGGCATACACTTTCAAAGTTGAGGCT
  TCCAACCTTCACCTTGACCACCGGTTTCACTCGGCGGGCCCTTTCAAAGACACAGCTACGGTGAAGATCA
  GCGTGCTGGACGTAGATGAGCCACCGGTTTTCAGCAAGCCGCTCTACACCATGGAGGTTTATGAAGACAC
  TCCGGTAGGGACCATCATTGGCGCTGTCACTGCTCAAGACCTGGATGTAGGCAGCAGTGCTGTTAGGTAC
  TTCATAGATTGGAAGAGTGATGGGGACAGCTACTTTACAATAGATGGAAATGAAGGAACCATCGCCACTA
  ATGAATTACTAGACAGAGAAAGCACTGCGCAGTATAATTTCTCCATAATTGCGAGTAAAGTTAGTAACCC
  TTTATTGACCAGCAAAGTCAATATACTGATTAATGTCTTAGATGTAAATGAATTTCCTCCAGAAATATCT
  GTGCCATATGAGACAGCCGTGTGTGAAAATGCCAAGCCAGGACAGATAATTCAGATAGTCAGTGCTGCAG
  ACCGAGATCTTTCACCTGCTGGGCAACAATTCTCCTTTAGATTATCACCTGAGGCTGCTATCAAACCAAA
  TTTTACAGTTCGTGACTTCAGAAACAACACAGCGGGGATTGAAACCCGAAGAAATGGATACAGCCGCAGG
  CAGCAAGAGTTGTATTTCCTCCCTGTTGTAATAGAAGACAGCAGCTACCCTGTCCAGAGCAGCACAAACA
  CAATGACTATTCGAGTCTGTAGATGTGACTCTGATGGCACCATCCTGTCTTGTAATGTGGAAGCAATTTT
  TCTACCTGTAGGACTTAGCACTGGGGCGTTGATTGCAATTCTACTATGCATTGTTATACTCTTAGCCATA
  GTTGTACTGTATGTAGCACTGCGAAGGCAGAAGAAAAAAGACACCCTGATGACCTCTAAAGAAGACATCA
  GAGACAACGTCATCCATTACGATGATGAAGGAGGTGGGGAGGAAGATACCCAGGCTTTCGACATCGGGGC
  TCTGAGAAACCCAAAAGTGATTGAGGAGAACAAAATTCGCAGGGATATAAAACCAGACTCTCTCTGTTTA
  CCTCGTCAGAGACCACCCATGGAAGATAACACAGACATAAGGGATTTCATTCATCAAAGGCTACAGGAAA
  ATGATGTGGATCCAACTGCCCCACCATACGATTCACTGGCCACATATGCCTACGAAGGGAGTGGGTCCGT
  GGCAGAGTCCCTCAGCTCTATAGACTCTCTCACCACAGAAGCCGACCAGGACTATGACTATCTGACAGAC
  TGGGGACCCCGCTTTAAAGTCTTGGCAGACATGTTTGGCGAAGAAGAGAGTTATAACCCTGATAAAGTCA
  CT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208156 representing NM_004061
  Red=Cloning site Green=Tags(s)
  
  MLTRNCLSLLLWVLFDGGLLTPLQPQPQQTLATEPRENVIHLPGQRSHFQRVKRGWVWNQFFVLEEYVGS
  EPQYVGKLHSDLDKGEGTVKYTLSGDGAGTVFTIDETTGDIHAIRSLDREEKPFYTLRAQAVDIETRKPL
  EPESEFIIKVQDINDNEPKFLDGPYVATVPEMSPVGAYVLQVKATDADDPTYGNSARVVYSILQGQPYFS
  IDPKTGVIRTALPNMDREVKEQYQVLIQAKDMGGQLGGLAGTTIVNITLTDVNDNPPRFPKSIFHLKVPE
  SSPIGSAIGRIRAVDPDFGQNAEIEYNIVPGDGGNLFDIVTDEDTQEGVIKLKKPLDFETKKAYTFKVEA
  SNLHLDHRFHSAGPFKDTATVKISVLDVDEPPVFSKPLYTMEVYEDTPVGTIIGAVTAQDLDVGSSAVRY
  FIDWKSDGDSYFTIDGNEGTIATNELLDRESTAQYNFSIIASKVSNPLLTSKVNILINVLDVNEFPPEIS
  VPYETAVCENAKPGQIIQIVSAADRDLSPAGQQFSFRLSPEAAIKPNFTVRDFRNNTAGIETRRNGYSRR
  QQELYFLPVVIEDSSYPVQSSTNTMTIRVCRCDSDGTILSCNVEAIFLPVGLSTGALIAILLCIVILLAI
  VVLYVALRRQKKKDTLMTSKEDIRDNVIHYDDEGGGEEDTQAFDIGALRNPKVIEENKIRRDIKPDSLCL
  PRQRPPMEDNTDIRDFIHQRLQENDVDPTAPPYDSLATYAYEGSGSVAESLSSIDSLTTEADQDYDYLTD
  WGPRFKVLADMFGEEESYNPDKVT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004061
• ORF Size: 2382 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004061.5
• RefSeq Size: 4164 bp
• RefSeq ORF: 2385 bp
• Locus ID: 1010
• UniProt ID: P55289
• Cytogenetics: 5p14.3
• Domains: Cadherin_C_term, CA
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a type II classical cadherin of the cadherin superfamily. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature cadherin protein. These integral membrane proteins mediate calcium-dependent cell-cell adhesion and are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a histidine-alanine-valine (HAV) cell adhesion recognition sequence specific to type I cadherins. This particular cadherin appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis. [provided by RefSeq, Nov 2015]