TUBB1 (NM_030773) Human Tagged ORF Clone
CAT#: RG207459
- TrueORF®
TUBB1 (tGFP-tagged) - Human tubulin, beta 1 (TUBB1)
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_030773" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | TUBB1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG207459 representing NM_030773
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTGAAATTGTCCATATTCAGATTGGCCAGTGTGGCAACCAGATCGGAGCCAAGTTCTGGGAGATGA TTGGTGAGGAACACGGGATCGACTTGGCTGGGAGCGACCGCGGGGCCTCGGCCTTGCAGCTGGAGAGAAT CAGCGTGTACTACAACGAAGCCTACGGTAGGAAATATGTGCCCCGAGCAGTCTTGGTGGACCTAGAACCT GGGACGATGGACAGCATTCGATCTAGCAAATTAGGAGCTCTCTTTCAACCCGACAGTTTTGTCCATGGTA ACTCTGGGGCTGGCAACAACTGGGCCAAAGGCCACTACACGGAGGGAGCCGAGCTGATCGAGAATGTCCT AGAGGTGGTGAGGCACGAGAGTGAGAGCTGTGACTGCCTGCAGGGCTTCCAGATCGTCCACTCCCTGGGC GGGGGCACAGGCTCCGGGATGGGCACTCTGCTCATGAACAAGATTAGAGAGGAGTACCCGGACCGGATCA TGAATTCCTTCAGCGTCATGCCTTCTCCCAAGGTGTCGGACACGGTGGTGGAGCCCTACAACGCGGTTCT GTCTATCCACCAGCTGATTGAGAATGCAGATGCCTGTTTCTGCATTGACAATGAGGCCCTCTATGACATC TGCTTCCGTACCCTGAAGCTGACGACACCCACCTATGGGGATCTCAACCACCTAGTGTCCTTGACCATGA GCGGCATAACCACCTCCCTCCGGTTCCCGGGTCAGCTCAACGCAGACCTGCGCAAGCTGGCGGTGAACAT GGTCCCCTTCCCCCGCCTGCACTTCTTTATGCCCGGCTTTGCCCCACTCACGGCCCAGGGCAGCCAGCAG TACCGAGCCCTCTCCGTGGCCGAGCTCACCCAGCAGATGTTCGATGCCCGCAATACCATGGCTGCCTGTG ACCTCCGCCGTGGCCGCTACCTCACAGTGGCCTGCATTTTCCGGGGCAAGATGTCCACCAAGGAAGTGGA CCAGCAACTGCTCTCCGTGCAGACCAGGAACAGCAGCTGCTTTGTGGAGTGGATTCCCAACAACGTCAAG GTGGCTGTCTGCGACATCCCGCCCCGGGGGCTGAGCATGGCCGCCACCTTCATTGGCAACAACACGGCCA TCCAAGAGATCTTTAATAGGGTCTCTGAGCATTTCTCAGCCATGTTCAAAAGGAAAGCTTTTGTGCACTG GTACACCAGCGAAGGGATGGACATAAACGAATTTGGGGAAGCTGAAAATAACATCCATGATTTGGTATCC GAGTACCAACAATTTCAAGATGCCAAAGCAGTTCTAGAGGAAGATGAAGAGGTCACGGAGGAGGCAGAAA TGGAGCCAGAAGATAAGGGACAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207459 representing NM_030773
Red=Cloning site Green=Tags(s) MREIVHIQIGQCGNQIGAKFWEMIGEEHGIDLAGSDRGASALQLERISVYYNEAYGRKYVPRAVLVDLEP GTMDSIRSSKLGALFQPDSFVHGNSGAGNNWAKGHYTEGAELIENVLEVVRHESESCDCLQGFQIVHSLG GGTGSGMGTLLMNKIREEYPDRIMNSFSVMPSPKVSDTVVEPYNAVLSIHQLIENADACFCIDNEALYDI CFRTLKLTTPTYGDLNHLVSLTMSGITTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTAQGSQQ YRALSVAELTQQMFDARNTMAACDLRRGRYLTVACIFRGKMSTKEVDQQLLSVQTRNSSCFVEWIPNNVK VAVCDIPPRGLSMAATFIGNNTAIQEIFNRVSEHFSAMFKRKAFVHWYTSEGMDINEFGEAENNIHDLVS EYQQFQDAKAVLEEDEEVTEEAEMEPEDKGH TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_030773 |
ORF Size | 1353 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_030773.2, NP_110400.1 |
RefSeq Size | 3496 bp |
RefSeq ORF | 1356 bp |
Locus ID | 81027 |
UniProt ID | Q9H4B7 |
Cytogenetics | 20q13.32 |
Domains | tubulin |
Protein Families | Druggable Genome |
Protein Pathways | Gap junction, Pathogenic Escherichia coli infection |
Gene Summary | This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.[provided by RefSeq, Jul 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207459 | TUBB1 (Myc-DDK-tagged)-Human tubulin, beta 1 (TUBB1) |
USD 457.00 |
|
RC207459L1 | Lenti ORF clone of Human tubulin, beta 1 (TUBB1), Myc-DDK-tagged |
USD 757.00 |
|
RC207459L2 | Lenti ORF clone of Human tubulin, beta 1 (TUBB1), mGFP tagged |
USD 757.00 |
|
RC207459L3 | Lenti ORF clone of Human tubulin, beta 1 (TUBB1), Myc-DDK-tagged |
USD 757.00 |
|
RC207459L4 | Lenti ORF clone of Human tubulin, beta 1 (TUBB1), mGFP tagged |
USD 757.00 |
|
SC109413 | TUBB1 (untagged)-Human tubulin, beta 1 (TUBB1) |
USD 457.00 |
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