C9orf96 (STKLD1) (NM_153710) Human Tagged ORF Clone

CAT#: RG207330

C9orf96 (tGFP-tagged) - Human chromosome 9 open reading frame 96 (C9orf96)

Product Images

Circular map for RG207330

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C9orf96
• Synonyms: C9orf96; SgK071; Sk521
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207330 representing NM_153710
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTGGGCCAGGGTCCAATCGCAGGCGCCCCACGCAGGGGGAGCGAGGCCCAGGGTCCCCCGGAGAGC
  CCATGGAGAAGTACCAGGTTTTGTACCAGCTGAATCCTGGGGCCTTGGGGGTGAACCTGGTGGTGGAGGA
  AATGGAAACCAAAGTCAAGCATGTGATAAAGCAGGTGGAATGCATGGATGACCATTACGCCAGTCAGGCC
  CTGGAGGAGCTGATGCCACTGCTGAAGCTGCGGCACGCCCACATCTCTGTGTACCAGGAGCTGTTCATCA
  CGTGGAATGGGGAGATCTCTTCTCTGTACCTCTGCCTGGTGATGGAGTTCAATGAGCTCAGCTTCCAGGA
  GGTCATTGAGGATAAGAGGAAGGCAAAGAAAATCATTGACTCTGAGTGGATGCAGAATGTGCTGGGCCAG
  GTGCTGGACGCGCTGGAATACCTGCACCATTTGGACATCATCCACAGGAATCTCAAACCCTCCAACATCA
  TCCTCATCAGCAGTGACCACTGCAAACTGCAGGACCTGAGTTCCAATGTGCTAATGACAGACAAAGCCAA
  ATGGAATATTCGTGCGGAGGAAGACCCCTTTCGTAAGTCCTGGATGGCCCCTGAAGCCCTCAACTTCTCC
  TTCAGCCAGAAATCAGACATCTGGTCCCTGGGCTGCATCATTCTGGACATGACCAGCTGCTCCTTCATGG
  ATGGCACAGAAGCCATGCATCTGCGGAAGTCCCTCCGCCAGAGCCCAGGCAGCCTGAAGGCCGTCCTGAA
  GACAATGGAGGAGAAGCAGATCCCGGATGTGGAAACCTTCAGGAATCTTCTGCCCTTGATGCTCCAGATC
  GACCCCTCGGATCGAATAACGATAAAGGACGTGGTGCACATCACCTTCTTGAGAGGCTCCTTCAAGTCCT
  CGTGCGTCTCTCTGACCCTGCACCGGCAGATGGTGCCTGCGTCCATCACCGACATGCTGTTAGAAGGCAA
  CGTGGCCAGCATTTTAGAGGTCATGCAGAAATTCTCTGGCTGGCCCGAAGTCCAGCTCAGGGCCATGAAG
  AGGCTTCTGAAAATGCCTGCAGATCAGCTAGGTCTGCCGTGGCCCCCGGAGCTGGTGGAGGTGGTGGTCA
  CGACCATGGAGCTACATGACAGGGTCCTCGATGTCCAGCTGTGTGCCTGCTCCCTGCTGCTGCACCTCCT
  GGGCCAAGCGCTGGTGCACCACCCGGAAGCCAAGGCTCCCTGCAACCAAGCCATCACCTCCACCCTGCTG
  AGTGCTCTTCAGAGCCACCCCGAGGAGGAGCCACTTCTTGTCATGGTCTACAGCCTGCTAGCCATCACCA
  CAACCCAGGAGTCAGAGTCACTGTCAGAGGAGCTGCAGAACGCTGGGCTGCTGGAGCACATCCTGGAGCA
  CCTCAACAGCTCCCTCGAAAGCAGGGACGTCTGCGCCAGCGGCCTGGGCCTGCTCTGGGCCCTCCTGCTG
  GACGGTATCATTGTGAACAAGGCCCCCTTGGAGAAGGTCCCGGACCTCATCAGCCAGGTGTTGGCCACCT
  ACCCTGCGGATGGGGAAATGGCAGAAGCCAGCTGCGGAGTCTTCTGGCTGCTGTCCCTGCTGGGCTGCAT
  CAAGGAGCAGCAGTTTGAACAAGTGGTGGCGCTGCTCCTGCAAAGCATCCGGCTGTGCCAGGACAGAGCC
  CTGCTGGTGAACAATGCCTACCGGGGACTGGCCAGCCTGGTGAAGGTGTCAGAGCTGGCGGCCTTCAAGG
  TGGTGGTGCAGGAGGAGGGCGGCAGTGGCCTCAGCCTCATCAAGGAGACCTACCAGCTCCACAGGGACGA
  CCCGGAGGTGGTGGAGAACGTGGGCATGCTGCTGGTCCACCTGGCTTCCTATGAGGAGATCCTGCCGGAG
  CTGGTGTCCAGTAGTATGAAGGCCCTGCTCCAGGAGATCAAGGAGCGCTTCACCTCCAGCCTGGTGAGTG
  ACAGCAGCGCCTTCAGCAAACCAGGCCTCCCTCCAGGTGGAAGCCCCCAGCTGGGGTGCACCACGTCTGG
  GGGACTGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207330 representing NM_153710
  Red=Cloning site Green=Tags(s)
  
  MLGPGSNRRRPTQGERGPGSPGEPMEKYQVLYQLNPGALGVNLVVEEMETKVKHVIKQVECMDDHYASQA
  LEELMPLLKLRHAHISVYQELFITWNGEISSLYLCLVMEFNELSFQEVIEDKRKAKKIIDSEWMQNVLGQ
  VLDALEYLHHLDIIHRNLKPSNIILISSDHCKLQDLSSNVLMTDKAKWNIRAEEDPFRKSWMAPEALNFS
  FSQKSDIWSLGCIILDMTSCSFMDGTEAMHLRKSLRQSPGSLKAVLKTMEEKQIPDVETFRNLLPLMLQI
  DPSDRITIKDVVHITFLRGSFKSSCVSLTLHRQMVPASITDMLLEGNVASILEVMQKFSGWPEVQLRAMK
  RLLKMPADQLGLPWPPELVEVVVTTMELHDRVLDVQLCACSLLLHLLGQALVHHPEAKAPCNQAITSTLL
  SALQSHPEEEPLLVMVYSLLAITTTQESESLSEELQNAGLLEHILEHLNSSLESRDVCASGLGLLWALLL
  DGIIVNKAPLEKVPDLISQVLATYPADGEMAEASCGVFWLLSLLGCIKEQQFEQVVALLLQSIRLCQDRA
  LLVNNAYRGLASLVKVSELAAFKVVVQEEGGSGLSLIKETYQLHRDDPEVVENVGMLLVHLASYEEILPE
  LVSSSMKALLQEIKERFTSSLVSDSSAFSKPGLPPGGSPQLGCTTSGGLE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153710
• ORF Size: 2040 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153710.2
• RefSeq Size: 2779 bp
• RefSeq ORF: 2043 bp
• Locus ID: 169436
• UniProt ID: Q8NE28
• Cytogenetics: 9q34.2
• Protein Families: Protein Kinase