ZNF714 (NM_182515) Human Tagged ORF Clone

CAT#: RG207240

ZNF714 (tGFP-tagged) - Human zinc finger protein 714 (ZNF714)

Product Images

Circular map for RG207240

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF714
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207240 representing NM_182515
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGTGATGTTAGAGAACTACAAAAACCTGGTCTTCTTGGCAGGTATTGCTGTCTCTAAGCAAGACC
  CGATCACCAGTCTAGAGCAAGAAAAAGAGCCCTGGAATATGAAGATATGTGAGATGGTGGATGAATCCCC
  AGCTATGTGTTCTTCTTTTACCAGAGACCTTTGGCCAGAGCAAGACATAAAAGATTCTTTTCAACAAGTG
  ATACTGAGAAGACATGGCAAATGTGAACATGAGAATTTACAGTTAAGAAAAGGCTCCGCAAATGTGGTTG
  AGTGTAAGGTGTACAAAAAAGGTTATAATGAACTAAACCAGTGTTTGACAACTACCCAGAGCAAAATATT
  TCCATGTGATAAATATATAAAAGTCTTTCATAAAATTTTCAATTCAAATAGACACAAGACAAGACATACT
  GGAGAGAAACCTTTCAAATGTAAAAAATGTGATGAATCATTTTGCATGCTTTTACACCTACATCAACATA
  AAAGAATTCATATTAGAGAGAATTCTTACCAATGTGAAGAATGTGACAAAGTGTTTAAGCGGTTCTCAAC
  CCTTACTAGACACAAGAGAGTTCATACTGGAGAGAAACCCTTCAAATGTGAAGAATGTGGCAAAGCTTTT
  AAGCACTCCTCAACCCTTACTACACATAAGATGATTCATACTGGAGAGAAACCCTACAGATGTGAAGAAT
  GTGGCAAAGCCTTCTACCATTCTTCACACCTTACTACACATAAGGTAATTCATACTGGAGAGAAGCCCTT
  CAAATGTGAAGAATGTGGTAAAGCTTTTAACCACCCTTCAGCCCTTACTACACATAAGTTCATTCATGTT
  AAAGAAAAACCCTACAAATGTGAAGAATGTGACAAAGCTTTTAACCGATTCTCATACCTTACTAAACATA
  AGATAATTCATTCTGGAGAGAAATCTTACAAATGTGAACAATGTGGCAAAGGCTTTAACTGGTCTTCAAC
  CCTTACAAAACATAAAAGAATTCATACTGGAGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCCTTT
  AATGTGTCTTCACACCTTACTACACATAAGATGATTCATACTGGAGAGAAACCCTACAAATGTGAAGAAT
  GTGGCAAAGCCTTTAACCACTCCTCAAAACTTACTATACATAAGATAATTCATACTGGAGAGAAACCTTA
  CAAATGTGAAGAATGTGGCAAAGCTTTTAACCAATCCTCAAACCTTACCAAACATAAGATAATTCATACT
  GGAGAGAAACTCTACAAATGTGAAGAATGTGGCAAAGCTTTTAACCGATCCTCAAACCTTACTACACATA
  AGAGAATTCACACTGGAGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCTTTCAACCGATCCTCAAA
  CCTTACTAAACATAACATAATTCATACTGGAGAGAAATCTTACAAATGTGAAGAATGTGGTAAAGCCTTT
  AACCAATCCTCAACTCTTACTAAACATAGGAAAATTCAGCAGGGCATGGTGGCTCATGCCTGTAATCCCA
  ACACTTTGAGAGGACTAGGTGAGCAGATCGCGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTAA
  AACCCCATCTCTACTAAAAATACAAAAATTTGCTGGGTGTGGTGGCAGGCGCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207240 representing NM_182515
  Red=Cloning site Green=Tags(s)
  
  MNVMLENYKNLVFLAGIAVSKQDPITSLEQEKEPWNMKICEMVDESPAMCSSFTRDLWPEQDIKDSFQQV
  ILRRHGKCEHENLQLRKGSANVVECKVYKKGYNELNQCLTTTQSKIFPCDKYIKVFHKIFNSNRHKTRHT
  GEKPFKCKKCDESFCMLLHLHQHKRIHIRENSYQCEECDKVFKRFSTLTRHKRVHTGEKPFKCEECGKAF
  KHSSTLTTHKMIHTGEKPYRCEECGKAFYHSSHLTTHKVIHTGEKPFKCEECGKAFNHPSALTTHKFIHV
  KEKPYKCEECDKAFNRFSYLTKHKIIHSGEKSYKCEQCGKGFNWSSTLTKHKRIHTGEKPYKCEECGKAF
  NVSSHLTTHKMIHTGEKPYKCEECGKAFNHSSKLTIHKIIHTGEKPYKCEECGKAFNQSSNLTKHKIIHT
  GEKLYKCEECGKAFNRSSNLTTHKRIHTGEKPYKCEECGKAFNRSSNLTKHNIIHTGEKSYKCEECGKAF
  NQSSTLTKHRKIQQGMVAHACNPNTLRGLGEQIARSGVQDQPGQHGKTPSLLKIQKFAGCGGRRL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182515
• ORF Size: 1665 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182515.4
• RefSeq Size: 8790 bp
• RefSeq ORF: 1668 bp
• Locus ID: 148206
• UniProt ID: Q96N38
• Cytogenetics: 19p12
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]