Ephrin B3 (EFNB3) (NM_001406) Human Tagged ORF Clone

CAT#: RG207228

EFNB3 (tGFP-tagged) - Human ephrin-B3 (EFNB3)

Product Images

Circular map for RG207228

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ephrin B3
• Synonyms: EFL6; EPLG8; LERK8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207228 representing NM_001406
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCCCCCCATTCTGGGCCGGGGGGCGTGCGAGTCGGGGCCCTGCTGCTGCTGGGGGTTTTGGGGC
  TGGTGTCTGGGCTCAGCCTGGAGCCTGTCTACTGGAACTCGGCGAATAAGAGGTTCCAGGCAGAGGGTGG
  TTATGTGCTGTACCCTCAGATCGGGGACCGGCTAGACCTGCTCTGCCCCCGGGCCCGGCCTCCTGGCCCT
  CACTCCTCTCCTAATTATGAGTTCTACAAGCTGTACCTGGTAGGGGGTGCTCAGGGCCGGCGCTGTGAGG
  CACCCCCTGCCCCAAACCTCCTTCTCACTTGTGATCGCCCAGACCTGGATCTCCGCTTCACCATCAAGTT
  CCAGGAGTATAGCCCTAATCTCTGGGGCCACGAGTTCCGCTCGCACCACGATTACTACATCATTGCCACA
  TCGGATGGGACCCGGGAGGGCCTGGAGAGCCTGCAGGGAGGTGTGTGCCTAACCAGAGGCATGAAGGTGC
  TTCTCCGAGTGGGACAAAGTCCCCGAGGAGGGGCTGTCCCCCGAAAACCTGTGTCTGAAATGCCCATGGA
  AAGAGACCGAGGGGCAGCCCACAGCCTGGAGCCTGGGAAGGAGAACCTGCCAGGTGACCCCACCAGCAAT
  GCAACCTCCCGGGGTGCTGAAGGCCCCCTGCCCCCTCCCAGCATGCCTGCAGTGGCTGGGGCAGCAGGGG
  GGCTGGCGCTGCTCTTGCTGGGCGTGGCAGGGGCTGGGGGTGCCATGTGTTGGCGGAGACGGCGGGCCAA
  GCCTTCGGAGAGTCGCCACCCTGGTCCTGGCTCCTTCGGGAGGGGAGGGTCTCTGGGCCTGGGGGGTGGA
  GGTGGGATGGGACCTCGGGAGGCTGAGCCTGGGGAGCTAGGGATAGCTCTGCGGGGTGGCGGGGCTGCAG
  ATCCCCCCTTCTGCCCCCACTATGAGAAGGTGAGTGGTGACTATGGGCATCCTGTGTATATCGTGCAGGA
  TGGGCCCCCCCAGAGCCCTCCAAACATCTACTACAAGGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207228 representing NM_001406
  Red=Cloning site Green=Tags(s)
  
  MGPPHSGPGGVRVGALLLLGVLGLVSGLSLEPVYWNSANKRFQAEGGYVLYPQIGDRLDLLCPRARPPGP
  HSSPNYEFYKLYLVGGAQGRRCEAPPAPNLLLTCDRPDLDLRFTIKFQEYSPNLWGHEFRSHHDYYIIAT
  SDGTREGLESLQGGVCLTRGMKVLLRVGQSPRGGAVPRKPVSEMPMERDRGAAHSLEPGKENLPGDPTSN
  ATSRGAEGPLPPPSMPAVAGAAGGLALLLLGVAGAGGAMCWRRRRAKPSESRHPGPGSFGRGGSLGLGGG
  GGMGPREAEPGELGIALRGGGAADPPFCPHYEKVSGDYGHPVYIVQDGPPQSPPNIYYKV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001406
• ORF Size: 1020 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001406.4
• RefSeq Size: 3236 bp
• RefSeq ORF: 1023 bp
• Locus ID: 1949
• UniProt ID: Q15768
• Cytogenetics: 17p13.1
• Domains: Ephrin
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: EFNB3, a member of the ephrin gene family, is important in brain development as well as in its maintenance. Moreover, since levels of EFNB3 expression were particularly high in several forebrain subregions compared to other brain subregions, it may play a pivotal role in forebrain function. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH Receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]