CLEC4D (NM_080387) Human Tagged ORF Clone

CAT#: RG207032

CLEC4D (tGFP-tagged) - Human C-type lectin domain family 4, member D (CLEC4D)

Product Images

Circular map for RG207032

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CLEC4D
• Synonyms: CD368; CLEC-6; CLEC6; CLECSF8; Dectin-3; MCL; MPCL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207032 representing NM_080387
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTAGAAAAACCTCAAAGTAAACTGGAAGGAGGCATGCATCCCCAGCTGATACCTTCGGTTATTG
  CTGTAGTTTTCATCTTACTTCTCAGTGTCTGTTTTATTGCAAGTTGTTTGGTGACTCATCACAACTTTTC
  ACGCTGTAAGAGAGGCACAGGAGTGCACAAGTTAGAGCACCATGCAAAGCTCAAATGCATCAAAGAGAAA
  TCAGAACTGAAAAGTGCTGAAGGGAGCACCTGGAACTGTTGTCCTATTGACTGGAGAGCCTTCCAGTCCA
  ACTGCTATTTTCCTCTTACTGACAACAAGACGTGGGCTGAGAGTGAAAGGAACTGTTCAGGGATGGGGGC
  CCATCTGATGACCATCAGCACGGAAGCTGAGCAGAACTTTATTATTCAGTTTCTGGATAGACGGCTTTCC
  TATTTCCTTGGACTTAGAGATGAGAATGCCAAAGGTCAGTGGCGTTGGGTGGACCAGACGCCATTTAACC
  CACGCAGAGTATTCTGGCATAAGAATGAACCCGACAACTCTCAGGGAGAAAACTGTGTTGTTCTTGTTTA
  TAACCAAGATAAATGGGCCTGGAATGATGTTCCTTGTAACTTTGAAGCAAGTAGGATTTGTAAAATACCT
  GGAACAACATTGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207032 representing NM_080387
  Red=Cloning site Green=Tags(s)
  
  MGLEKPQSKLEGGMHPQLIPSVIAVVFILLLSVCFIASCLVTHHNFSRCKRGTGVHKLEHHAKLKCIKEK
  SELKSAEGSTWNCCPIDWRAFQSNCYFPLTDNKTWAESERNCSGMGAHLMTISTEAEQNFIIQFLDRRLS
  YFLGLRDENAKGQWRWVDQTPFNPRRVFWHKNEPDNSQGENCVVLVYNQDKWAWNDVPCNFEASRICKIP
  GTTLN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080387
• ORF Size: 645 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080387.5
• RefSeq Size: 1973 bp
• RefSeq ORF: 648 bp
• Locus ID: 338339
• UniProt ID: Q8WXI8
• Cytogenetics: 12p13.31
• Domains: CLECT
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]